A novel monoclonal antibody (OPD4) recognizing a helper/inducer T cell subset. Its application to paraffin-embedded tissues

Tadashi Yoshino, H. Mukuzono, H. Aoki, K. Takahashi, T. Takeuchi, I. Kubonishi, Y. Ohtsuki, M. Motoi, T. Akagi

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A novel monoclonal antibody (MAb), OPD4, reactive with a helper/inducer (H/I) subset of T cells in formalin-fixed, paraffin-embedded tissue sections, has been identified through immunization with an activated H/I T cell line, namely DL40. The antibody is an IgG1 antibody and it recognizes an antigen with a molecular weight of 200 kd, corresponding to that of leukocyte common antigen. OPD4+/CD4+ T cells provided better help for pokeweed mitogen-stimulated polyclonal IgG production than OPD4-/CD4+ T cells. OPD4 recognized the H/I T cell subset even in paraffin-embedded tissue sections, but did not recognize nonhematopoietic cells, suppressor/cytotoxic T cells, B cells, monocytes in the peripheral blood, or other normal hematopoietic cells as examined by the flow cytometeric and immunoperoxidase methods. Besides the lymphoid cells, OPD4 reacted with a number of bistiocytes (epithelioid cells) in tissues from sarcoidosis and tuberculosis. For the neoplastic lesions, OPD4 reacted with approximately half of the cases of T cell lymphomas. Consequently, OPD4 may be useful for the diagnosis and study of malignant lymphomas and other related lesions.

Original languageEnglish
Pages (from-to)1339-1346
Number of pages8
JournalAmerican Journal of Pathology
Volume134
Issue number6
Publication statusPublished - 1989

Fingerprint

T-Lymphocyte Subsets
Helper-Inducer T-Lymphocytes
Paraffin
Monoclonal Antibodies
T-Lymphocytes
Immunoglobulin G
CD45 Antigens
Pokeweed Mitogens
Epithelioid Cells
Antibodies
T-Cell Lymphoma
Sarcoidosis
Formaldehyde
Monocytes
Immunization
Lymphoma
Tuberculosis
B-Lymphocytes
Molecular Weight
Lymphocytes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Yoshino, T., Mukuzono, H., Aoki, H., Takahashi, K., Takeuchi, T., Kubonishi, I., ... Akagi, T. (1989). A novel monoclonal antibody (OPD4) recognizing a helper/inducer T cell subset. Its application to paraffin-embedded tissues. American Journal of Pathology, 134(6), 1339-1346.

A novel monoclonal antibody (OPD4) recognizing a helper/inducer T cell subset. Its application to paraffin-embedded tissues. / Yoshino, Tadashi; Mukuzono, H.; Aoki, H.; Takahashi, K.; Takeuchi, T.; Kubonishi, I.; Ohtsuki, Y.; Motoi, M.; Akagi, T.

In: American Journal of Pathology, Vol. 134, No. 6, 1989, p. 1339-1346.

Research output: Contribution to journalArticle

Yoshino, T, Mukuzono, H, Aoki, H, Takahashi, K, Takeuchi, T, Kubonishi, I, Ohtsuki, Y, Motoi, M & Akagi, T 1989, 'A novel monoclonal antibody (OPD4) recognizing a helper/inducer T cell subset. Its application to paraffin-embedded tissues', American Journal of Pathology, vol. 134, no. 6, pp. 1339-1346.
Yoshino, Tadashi ; Mukuzono, H. ; Aoki, H. ; Takahashi, K. ; Takeuchi, T. ; Kubonishi, I. ; Ohtsuki, Y. ; Motoi, M. ; Akagi, T. / A novel monoclonal antibody (OPD4) recognizing a helper/inducer T cell subset. Its application to paraffin-embedded tissues. In: American Journal of Pathology. 1989 ; Vol. 134, No. 6. pp. 1339-1346.
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