A novel method to detect 3D mandibular changes related to soft-diet feeding

Kana Kono, Chihiro Tanikawa, Takeshi Yanagita, Hiroshi Kamioka, Takashi Yamashiro

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Craniofacial morphology varies among individuals, which is regulated by the interaction between genes and the environment. Soft-diet feeding is a widely-used experimental model for studying the association between the skeletal morphology and muscle-related loading on the bone. Traditionally, these studies have been based on linear and angular measurements provided on two-dimensional (2D) radiographs in the lateral view. However, 2D observation is based on simplification of the anatomical structures and cannot detect three-dimensional (3D) changes in detail. In this study, we newly developed amodified surface-based analysis withmicro-3D computed tomography (CT) to examine and detect the 3D changes in the mandible associated with soft-diet feeding. Mice at 3 weeks of age were fed a powdered soft-diet (SD) or hard-diet (HD) of regular rodent pellets until 9 weeks of age. Micro-CT images were taken at age 9 weeks to reconstruct the anatomical architecture images. A computer-generated averaged mandible was superimposed to directly visualize the morphological phenotypes. Gross observation revealed the apparent changes at the posterior body of the mandible, the angular process and the condyle between HD and SD mice. Significant differences in the mapping indicated the regions of significant displacement in the SD mice over the averaged 3D image of the HD mice. This map revealed that vertical displacement was most evident in 3D changes. We also noted a combination of vertical, transverse and anteroposterior directions of displacement in the condylar growth, resulting in complicated shape changes in the whole condylar process in SD mice. In contrast, transverse displacement was more significant in the coronoid process. The map analysis further showed the significant outward displacement of the inner surface of the alveolar process, which consequently resulted in thinning of the alveolar process.

Original languageEnglish
Article number567
JournalFrontiers in Physiology
Volume8
Issue numberAUG
DOIs
Publication statusPublished - Aug 14 2017

Fingerprint

Diet
Mandible
Alveolar Process
Tomography
Observation
Bone and Bones
Gene-Environment Interaction
Rodentia
Skeletal Muscle
Theoretical Models
Phenotype
Growth

Keywords

  • 3D
  • CT
  • Mandible
  • Mice
  • Morphological change
  • Soft food

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

A novel method to detect 3D mandibular changes related to soft-diet feeding. / Kono, Kana; Tanikawa, Chihiro; Yanagita, Takeshi; Kamioka, Hiroshi; Yamashiro, Takashi.

In: Frontiers in Physiology, Vol. 8, No. AUG, 567, 14.08.2017.

Research output: Contribution to journalArticle

Kono, Kana ; Tanikawa, Chihiro ; Yanagita, Takeshi ; Kamioka, Hiroshi ; Yamashiro, Takashi. / A novel method to detect 3D mandibular changes related to soft-diet feeding. In: Frontiers in Physiology. 2017 ; Vol. 8, No. AUG.
@article{7652dac9846546d7a528b92bdc4a1637,
title = "A novel method to detect 3D mandibular changes related to soft-diet feeding",
abstract = "Craniofacial morphology varies among individuals, which is regulated by the interaction between genes and the environment. Soft-diet feeding is a widely-used experimental model for studying the association between the skeletal morphology and muscle-related loading on the bone. Traditionally, these studies have been based on linear and angular measurements provided on two-dimensional (2D) radiographs in the lateral view. However, 2D observation is based on simplification of the anatomical structures and cannot detect three-dimensional (3D) changes in detail. In this study, we newly developed amodified surface-based analysis withmicro-3D computed tomography (CT) to examine and detect the 3D changes in the mandible associated with soft-diet feeding. Mice at 3 weeks of age were fed a powdered soft-diet (SD) or hard-diet (HD) of regular rodent pellets until 9 weeks of age. Micro-CT images were taken at age 9 weeks to reconstruct the anatomical architecture images. A computer-generated averaged mandible was superimposed to directly visualize the morphological phenotypes. Gross observation revealed the apparent changes at the posterior body of the mandible, the angular process and the condyle between HD and SD mice. Significant differences in the mapping indicated the regions of significant displacement in the SD mice over the averaged 3D image of the HD mice. This map revealed that vertical displacement was most evident in 3D changes. We also noted a combination of vertical, transverse and anteroposterior directions of displacement in the condylar growth, resulting in complicated shape changes in the whole condylar process in SD mice. In contrast, transverse displacement was more significant in the coronoid process. The map analysis further showed the significant outward displacement of the inner surface of the alveolar process, which consequently resulted in thinning of the alveolar process.",
keywords = "3D, CT, Mandible, Mice, Morphological change, Soft food",
author = "Kana Kono and Chihiro Tanikawa and Takeshi Yanagita and Hiroshi Kamioka and Takashi Yamashiro",
year = "2017",
month = "8",
day = "14",
doi = "10.3389/fphys.2017.00567",
language = "English",
volume = "8",
journal = "Frontiers in Physiology",
issn = "1664-042X",
publisher = "Frontiers Research Foundation",
number = "AUG",

}

TY - JOUR

T1 - A novel method to detect 3D mandibular changes related to soft-diet feeding

AU - Kono, Kana

AU - Tanikawa, Chihiro

AU - Yanagita, Takeshi

AU - Kamioka, Hiroshi

AU - Yamashiro, Takashi

PY - 2017/8/14

Y1 - 2017/8/14

N2 - Craniofacial morphology varies among individuals, which is regulated by the interaction between genes and the environment. Soft-diet feeding is a widely-used experimental model for studying the association between the skeletal morphology and muscle-related loading on the bone. Traditionally, these studies have been based on linear and angular measurements provided on two-dimensional (2D) radiographs in the lateral view. However, 2D observation is based on simplification of the anatomical structures and cannot detect three-dimensional (3D) changes in detail. In this study, we newly developed amodified surface-based analysis withmicro-3D computed tomography (CT) to examine and detect the 3D changes in the mandible associated with soft-diet feeding. Mice at 3 weeks of age were fed a powdered soft-diet (SD) or hard-diet (HD) of regular rodent pellets until 9 weeks of age. Micro-CT images were taken at age 9 weeks to reconstruct the anatomical architecture images. A computer-generated averaged mandible was superimposed to directly visualize the morphological phenotypes. Gross observation revealed the apparent changes at the posterior body of the mandible, the angular process and the condyle between HD and SD mice. Significant differences in the mapping indicated the regions of significant displacement in the SD mice over the averaged 3D image of the HD mice. This map revealed that vertical displacement was most evident in 3D changes. We also noted a combination of vertical, transverse and anteroposterior directions of displacement in the condylar growth, resulting in complicated shape changes in the whole condylar process in SD mice. In contrast, transverse displacement was more significant in the coronoid process. The map analysis further showed the significant outward displacement of the inner surface of the alveolar process, which consequently resulted in thinning of the alveolar process.

AB - Craniofacial morphology varies among individuals, which is regulated by the interaction between genes and the environment. Soft-diet feeding is a widely-used experimental model for studying the association between the skeletal morphology and muscle-related loading on the bone. Traditionally, these studies have been based on linear and angular measurements provided on two-dimensional (2D) radiographs in the lateral view. However, 2D observation is based on simplification of the anatomical structures and cannot detect three-dimensional (3D) changes in detail. In this study, we newly developed amodified surface-based analysis withmicro-3D computed tomography (CT) to examine and detect the 3D changes in the mandible associated with soft-diet feeding. Mice at 3 weeks of age were fed a powdered soft-diet (SD) or hard-diet (HD) of regular rodent pellets until 9 weeks of age. Micro-CT images were taken at age 9 weeks to reconstruct the anatomical architecture images. A computer-generated averaged mandible was superimposed to directly visualize the morphological phenotypes. Gross observation revealed the apparent changes at the posterior body of the mandible, the angular process and the condyle between HD and SD mice. Significant differences in the mapping indicated the regions of significant displacement in the SD mice over the averaged 3D image of the HD mice. This map revealed that vertical displacement was most evident in 3D changes. We also noted a combination of vertical, transverse and anteroposterior directions of displacement in the condylar growth, resulting in complicated shape changes in the whole condylar process in SD mice. In contrast, transverse displacement was more significant in the coronoid process. The map analysis further showed the significant outward displacement of the inner surface of the alveolar process, which consequently resulted in thinning of the alveolar process.

KW - 3D

KW - CT

KW - Mandible

KW - Mice

KW - Morphological change

KW - Soft food

UR - http://www.scopus.com/inward/record.url?scp=85027466561&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85027466561&partnerID=8YFLogxK

U2 - 10.3389/fphys.2017.00567

DO - 10.3389/fphys.2017.00567

M3 - Article

AN - SCOPUS:85027466561

VL - 8

JO - Frontiers in Physiology

JF - Frontiers in Physiology

SN - 1664-042X

IS - AUG

M1 - 567

ER -