A novel method of preserving cardiac grafts using a hydrogen-rich water bath

Kentaro Noda, Norihisa Shigemura, Yugo Tanaka, Tomohiro Kawamura, Sang Hyun Lim, Kenichi Kokubo, Timothy R. Billiar, Christian A. Bermudez, Hirosuke Kobayashi, Atsunori Nakao

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: Exogenously administered hydrogen exerts cytoprotective effects through anti-oxidant, anti-inflammatory, and anti-apoptotic mechanisms in various disease settings, including organ transplantation. Our objective in this study was to evaluate the efficacy of a novel cold storage device equipped with a hydrogen-rich water bath. Methods: The study used an established rat heterotopic transplantation model. Syngeneic heart grafts from elderly donors (60- to 70-week-old Lewis rats) or allografts from adult donors (12-week-old Brown Norway rats) were exposed to prolonged cold preservation. The cardiac grafts were stored in plastic bags containing Celsior, which were immersed in the cold water bath equipped with an electrolyzer to saturate the water with hydrogen. The cardiac grafts then were heterotopically engrafted into Lewis rat recipients. Results: In both experimental settings, serum troponin I and creatine phosphokinase were markedly elevated 3 hours after reperfusion in the control grafts without hydrogen treatment. The grafts exhibited prominent inflammatory responses, including neutrophil infiltration and the upregulation of messenger RNAs for pro-inflammatory cytokines and chemokines. Myocardial injury and inflammatory events were significantly attenuated by organ storage in the hydrogen-rich water bath. The grafts stored using the hydrogen-rich water bath also exhibited less mitochondrial damage and a higher adenosine triphosphate content. Conclusions: Hydrogen delivery to cardiac grafts during cold preservation using a novel hydrogen-supplemented water bath efficiently ameliorated myocardial injury due to cold ischemia and reperfusion. This new device to saturate organs with hydrogen during cold storage merits further investigation for possible therapeutic and preventative use during transplantation.

Original languageEnglish
Pages (from-to)241-250
Number of pages10
JournalJournal of Heart and Lung Transplantation
Volume32
Issue number2
DOIs
Publication statusPublished - Feb 2013
Externally publishedYes

Fingerprint

Baths
Hydrogen
Transplants
Water
Reperfusion
Tissue Donors
Heterotopic Transplantation
Cold Ischemia
Equipment and Supplies
Troponin I
Neutrophil Infiltration
Wounds and Injuries
Organ Transplantation
Therapeutic Uses
Creatine Kinase
Chemokines
Oxidants
Plastics
Allografts
Anti-Inflammatory Agents

Keywords

  • cardiac transplantation
  • hydrogen
  • mitochondria
  • new device
  • organ preservation

ASJC Scopus subject areas

  • Transplantation
  • Cardiology and Cardiovascular Medicine
  • Pulmonary and Respiratory Medicine
  • Surgery

Cite this

A novel method of preserving cardiac grafts using a hydrogen-rich water bath. / Noda, Kentaro; Shigemura, Norihisa; Tanaka, Yugo; Kawamura, Tomohiro; Hyun Lim, Sang; Kokubo, Kenichi; Billiar, Timothy R.; Bermudez, Christian A.; Kobayashi, Hirosuke; Nakao, Atsunori.

In: Journal of Heart and Lung Transplantation, Vol. 32, No. 2, 02.2013, p. 241-250.

Research output: Contribution to journalArticle

Noda, K, Shigemura, N, Tanaka, Y, Kawamura, T, Hyun Lim, S, Kokubo, K, Billiar, TR, Bermudez, CA, Kobayashi, H & Nakao, A 2013, 'A novel method of preserving cardiac grafts using a hydrogen-rich water bath', Journal of Heart and Lung Transplantation, vol. 32, no. 2, pp. 241-250. https://doi.org/10.1016/j.healun.2012.11.004
Noda, Kentaro ; Shigemura, Norihisa ; Tanaka, Yugo ; Kawamura, Tomohiro ; Hyun Lim, Sang ; Kokubo, Kenichi ; Billiar, Timothy R. ; Bermudez, Christian A. ; Kobayashi, Hirosuke ; Nakao, Atsunori. / A novel method of preserving cardiac grafts using a hydrogen-rich water bath. In: Journal of Heart and Lung Transplantation. 2013 ; Vol. 32, No. 2. pp. 241-250.
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