A novel locus for clubroot resistance in Brassica rapa and its linkage markers

M. Hirai, Taro Harada, N. Kubo, M. Tsukada, K. Suwabe, S. Matsumoto

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

An inbred turnip (Brassica rapa syn. campestris) line, N-WMR-3, which carries the trait of clubroot resistance (CR) from a European turnip, Milan White, was crossed with a clubroot-susceptible doubled haploid line, A9709. A segregating F3 population was obtained by single-seed descent of F2 plants and used for a genetic analysis. Segregation of CR in the F3 population suggested that CR is controlled by a major gene. Two RAPD markers, OPC11-1 and OPC11-2, were obtained as candidates of linkage markers by bulked segregant analysis. These were converted to sequence-tagged site markers, by cloning and sequencing of the polymorphic bands, and named OPC11-1S and OPC11-2S, respectively. The specific primer pairs for OPC11-1S amplified a clear dominant band, while the primer pairs for OPC11-2S resulted in co-dominant bands. Frequency distributions and statistical analyses indicate the presence of a major dominant CR gene linked to these two markers. The present marker for CR was independent of the previously found CR loci, Crr1 and Crr2. Genotypic distribution and statistical analyses did not show any evidence of CR alleles on Crr1 and Crr2 loci in N-WMR-3. The present study clearly demonstrates that B. rapa has at least three CR loci. Therefore, the new CR locus was named Crr3. The present locus may be useful in breeding CR Chinese cabbage cultivars to overcome the decay of present CR cultivars.

Original languageEnglish
Pages (from-to)639-643
Number of pages5
JournalTheoretical And Applied Genetics
Volume108
Issue number4
DOIs
Publication statusPublished - Feb 2004
Externally publishedYes

Fingerprint

Statistical Distributions
Brassica napus
Brassica rapa
linkage (genetics)
Sequence Tagged Sites
loci
Haploidy
Brassica
Population
Genes
Breeding
Organism Cloning
Seeds
Alleles
turnips
clubroot
Chinese cabbage
doubled haploids
cultivars
major genes

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Cite this

A novel locus for clubroot resistance in Brassica rapa and its linkage markers. / Hirai, M.; Harada, Taro; Kubo, N.; Tsukada, M.; Suwabe, K.; Matsumoto, S.

In: Theoretical And Applied Genetics, Vol. 108, No. 4, 02.2004, p. 639-643.

Research output: Contribution to journalArticle

Hirai, M. ; Harada, Taro ; Kubo, N. ; Tsukada, M. ; Suwabe, K. ; Matsumoto, S. / A novel locus for clubroot resistance in Brassica rapa and its linkage markers. In: Theoretical And Applied Genetics. 2004 ; Vol. 108, No. 4. pp. 639-643.
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