A novel link between chloroplast development and stress response lessoned by leaf-variegated mutant

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Recessive mutations are known to give rise to cell lineage-type leaf variegation that forms green and white sectors due to arrested chloroplast development. The yellow variegated 2 (var2) mutant in Arabidopsis thaliana has been studied as a typical leaf-variegated mutant whose defect results from the lack of FtsH2 metalloprotease in chloroplasts. To understand physiological properties of variegated sectors, gene expression profiles were investigated between green and white sectors of var2 leaves. Consistent with impaired thylakoid formation, a substantial number of genes related to photosynthesis and chloroplast functions were repressed in white sectors. In addition, many genes were up-regulated in white sectors. Since var2 leaves suffer from photooxidative stress and accumulate high levels of reactive oxygen species (ROS) due to compromised Photosystem II repair, we focused ROS scavenging genes such as Cu/Zn superoxide dismutase 2 (CSD2). Activation of CSD2 was specific to white sectors and appeared to be under the control of copper availability. A lack of thylakoid membranes in white sectors perhaps leads to excess free copper and iron conditions, thus white sectors mimic a copper sufficient condition. We infer that CSD2 acts not only on ROS detoxification but also on copper buffering. Interestingly, an up-regulation of Cu/Zn SOD was commonly observed in various variegated leaves. Our findings thus highlight the crucial role for the control of oxidative stress and free metals in variegated leaf sectors.

Original languageEnglish
Title of host publicationAdvanced Topics in Science and Technology in China
PublisherSpringer
Pages669-673
Number of pages5
DOIs
Publication statusPublished - Jan 1 2013

Publication series

NameAdvanced Topics in Science and Technology in China
ISSN (Print)1995-6819
ISSN (Electronic)1995-6827

Fingerprint

Copper
Reactive Oxygen Species
Genes
Oxygen
Detoxification
Oxidative stress
Photosystem II Protein Complex
Photosynthesis
Scavenging
Metalloproteases
Gene expression
Repair
Iron
Metals
Chemical activation
Availability
Membranes
Defects
Superoxide Dismutase
superoxide dismutase 2

Keywords

  • Chloroplast development
  • FtsH
  • Leaf variegation
  • Photooxidative stress

ASJC Scopus subject areas

  • Engineering(all)
  • Chemical Engineering(all)
  • General

Cite this

Sakamoto, W., Miura, E., & Kato, Y. (2013). A novel link between chloroplast development and stress response lessoned by leaf-variegated mutant. In Advanced Topics in Science and Technology in China (pp. 669-673). (Advanced Topics in Science and Technology in China). Springer. https://doi.org/10.1007/978-3-642-32034-7_145

A novel link between chloroplast development and stress response lessoned by leaf-variegated mutant. / Sakamoto, Wataru; Miura, Eiko; Kato, Yusuke.

Advanced Topics in Science and Technology in China. Springer, 2013. p. 669-673 (Advanced Topics in Science and Technology in China).

Research output: Chapter in Book/Report/Conference proceedingChapter

Sakamoto, W, Miura, E & Kato, Y 2013, A novel link between chloroplast development and stress response lessoned by leaf-variegated mutant. in Advanced Topics in Science and Technology in China. Advanced Topics in Science and Technology in China, Springer, pp. 669-673. https://doi.org/10.1007/978-3-642-32034-7_145
Sakamoto W, Miura E, Kato Y. A novel link between chloroplast development and stress response lessoned by leaf-variegated mutant. In Advanced Topics in Science and Technology in China. Springer. 2013. p. 669-673. (Advanced Topics in Science and Technology in China). https://doi.org/10.1007/978-3-642-32034-7_145
Sakamoto, Wataru ; Miura, Eiko ; Kato, Yusuke. / A novel link between chloroplast development and stress response lessoned by leaf-variegated mutant. Advanced Topics in Science and Technology in China. Springer, 2013. pp. 669-673 (Advanced Topics in Science and Technology in China).
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