A novel Jun N-terminal kinase (JNK)-binding protein that enhances the activation of JNK by MEK kinase 1 and TGF-β-activated kinase 1

Satoru Koyano, Michihiko Ito, Nobuhiko Takamatsu, Tadayoshi Shiba, Ken Ichi Yamamoto, Katsuji Yoshioka

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We have identified a novel Jun N-terminal kinase (JNK)-binding protein, termed JNKBP1, and examined its binding affinity for JNK1, JNK2, JNK3, and extracellular signal-regulated kinase 2 (ERK2) in COS-7 cells. JNKBP1 preferentially interacted with the JNKs, but not with ERK2. Furthermore, we investigated the effect of overexpressing JNKBP1 on the JNK and ERK signaling pathways in COS-7 cells. JNKBP1 alone had only a marginal effect on JNK activity. However, the activation of JNK by MEK kinase 1 and TGF-β-activated kinase 1 was significantly enhanced in the presence of JNKBP1. In contrast, JNKBP1 had no or very little effect on the ERK signaling pathway. These results suggest that JNKBP1 functions to facilitate the specific and efficient activation of the JNK signaling pathways. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)385-388
Number of pages4
JournalFEBS Letters
Volume457
Issue number3
DOIs
Publication statusPublished - Sep 3 1999
Externally publishedYes

Keywords

  • Jun N-terminal kinase
  • Mitogen-activated protein kinase
  • Signal transduction
  • Stress-activated protein kinase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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