A novel human cancer culture model for the study of prostate cancer

Y. Yasunaga, Keiichiro Nakamura, D. Ko, S. Srivastava, J. W. Moul, I. A. Sesterhenn, D. G. McLeod, J. S. Rhim

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Research into molecular and genetic mechanisms underlying prostate carcinogenesis would be greatly advanced by in vitro models of prostate tumors representing primary tumors. We have successfully established an immortalized human prostate epithelial (HPE) cell culture derived from a primary tumor with telomerase. The actively proliferating early passaged RC-58T cells were transduced through infection with a retrovirus vector expressing the human telomerase catalytic subunit (hTERT). A high level of telomerase was detected in RC-58T/hTERT cells but not RC-58T cells. RC-58T/hTERT cells are currently growing well at passage 50, whereas RC-58T cells senesced at passage 7. RC-58T/hTERT cells exhibit transformed morphology. More importantly, these immortalized cells showed anchorage-independent growth as they formed colonies in soft agar and grew above the agar layer. Expression of androgen-regulated prostate specific gene NKX3.1 and epithelial specific cytokeratin 8 (CK8) but not prostate specific antigen (PSA) and androgen receptor was detected in RC-58T/hTERT cells. Prostate stem cell antigen (PSCA) and p16 were also expressed in this cell line. RC-58T/hTERT cells showed growth inhibition when exposed to retinoic acid and transforming growth factor (TGF)-β1 known potent inhibitors of prostate epithelial cell growth. A number of chromosome alterations were observed including the loss of chromosomes Y, 3p, 10p, 17p, 18q and the gain of chromosomes 16 and 20. These results demonstrate that this primary tumor-derived HPE cell line retained its transformed phenotypes and should allow studies to elucidate molecular and genetic alterations involved in prostate cancer. This is the first documented case of an established human prostate cancer cell line from a primary tumor of a prostate cancer patient with telomerase.

Original languageEnglish
Pages (from-to)8036-8041
Number of pages6
JournalOncogene
Volume20
Issue number55
DOIs
Publication statusPublished - Nov 29 2001
Externally publishedYes

Fingerprint

Telomerase
Prostatic Neoplasms
Prostate
Neoplasms
Epithelial Cells
Cell Line
Agar
Molecular Biology
Growth
Chromosomes, Human, Pair 20
Keratin-8
Chromosomes, Human, Pair 16
Antigen Receptors
Y Chromosome
Androgen Receptors
Transforming Growth Factors
Retroviridae
Prostate-Specific Antigen
Tretinoin
Androgens

Keywords

  • Human prostate cancer cell line
  • Immortalization
  • Telomerase

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Yasunaga, Y., Nakamura, K., Ko, D., Srivastava, S., Moul, J. W., Sesterhenn, I. A., ... Rhim, J. S. (2001). A novel human cancer culture model for the study of prostate cancer. Oncogene, 20(55), 8036-8041. https://doi.org/10.1038/sj/onc/1205002

A novel human cancer culture model for the study of prostate cancer. / Yasunaga, Y.; Nakamura, Keiichiro; Ko, D.; Srivastava, S.; Moul, J. W.; Sesterhenn, I. A.; McLeod, D. G.; Rhim, J. S.

In: Oncogene, Vol. 20, No. 55, 29.11.2001, p. 8036-8041.

Research output: Contribution to journalArticle

Yasunaga, Y, Nakamura, K, Ko, D, Srivastava, S, Moul, JW, Sesterhenn, IA, McLeod, DG & Rhim, JS 2001, 'A novel human cancer culture model for the study of prostate cancer', Oncogene, vol. 20, no. 55, pp. 8036-8041. https://doi.org/10.1038/sj/onc/1205002
Yasunaga Y, Nakamura K, Ko D, Srivastava S, Moul JW, Sesterhenn IA et al. A novel human cancer culture model for the study of prostate cancer. Oncogene. 2001 Nov 29;20(55):8036-8041. https://doi.org/10.1038/sj/onc/1205002
Yasunaga, Y. ; Nakamura, Keiichiro ; Ko, D. ; Srivastava, S. ; Moul, J. W. ; Sesterhenn, I. A. ; McLeod, D. G. ; Rhim, J. S. / A novel human cancer culture model for the study of prostate cancer. In: Oncogene. 2001 ; Vol. 20, No. 55. pp. 8036-8041.
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