A novel histidine-rich CPx-ATPase from the filamentous cyanobacterium Oscillatoria brevis related to multiple-heavy-metal cotolerance

Liu Tong, Susumu Nakashima, Mineo Shibasaka, Maki Katsuhara, Kunihiro Kasamo

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A novel gene related to heavy-metal transport was cloned and identified from the filamentous cyanobacterium Oscillatoria brevis. Sequence analysis of the gene (the Bxal gene) showed that its product possessed high homology with heavy-metal transport CPx-ATPases. The CPC motif, which is proposed to form putative cation transduction channel, was found in the sixth transmembrane helix. However, instead of the CXXC motif that is present in the N termini of most metal transport CPx-ATPases, Bxal contains a unique Cys-Cys (CC) sequence element and histidine-rich motifs as a putative metal binding site. Northern blotting and real-time quantitative reverse transcription-PCR showed that expression of Bxa1 mRNA was induced in vivo by both monovalent (Cu+ and Ag+) and divalent (Zn2+ and Cd2+) heavy-metal ions at similar levels. Experiments on heavy-metal tolerance in Escherichia coli with recombinant Bxal demonstrated that Bxal conferred resistance to both monovalent and divalent heavy metals. This is the first report of a CPx-ATPase responsive to both monovalent and divalent heavy metals.

Original languageEnglish
Pages (from-to)5027-5035
Number of pages9
JournalJournal of Bacteriology
Volume184
Issue number18
DOIs
Publication statusPublished - Sep 2002

Fingerprint

Oscillatoria
Cyanobacteria
Heavy Metals
Histidine
Adenosine Triphosphatases
cysteinylcysteine
Metals
Genes
Heavy Ions
Northern Blotting
Reverse Transcription
Sequence Analysis
Cations
Binding Sites
Escherichia coli
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

A novel histidine-rich CPx-ATPase from the filamentous cyanobacterium Oscillatoria brevis related to multiple-heavy-metal cotolerance. / Tong, Liu; Nakashima, Susumu; Shibasaka, Mineo; Katsuhara, Maki; Kasamo, Kunihiro.

In: Journal of Bacteriology, Vol. 184, No. 18, 09.2002, p. 5027-5035.

Research output: Contribution to journalArticle

Tong, Liu ; Nakashima, Susumu ; Shibasaka, Mineo ; Katsuhara, Maki ; Kasamo, Kunihiro. / A novel histidine-rich CPx-ATPase from the filamentous cyanobacterium Oscillatoria brevis related to multiple-heavy-metal cotolerance. In: Journal of Bacteriology. 2002 ; Vol. 184, No. 18. pp. 5027-5035.
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