A novel gene (retinovin) expressed selectively in the early stage of chick retinal development

Masako Itami, Toshihiko Matsuo, Hiroshi Ohtsuki

Research output: Contribution to journalArticle

Abstract

To understand molecular mechanisms of retinal development, genes expressed selectively only in the early stage of retinal development were isolated by subtractive hybridization based on suppression polymerase chain reaction. The retina has no layered structure in 7-day chick embryos, in contrast with the fully developed multilayered structure of neurons in 15-day embryos. The subtraction between cDNA derived from retinal tissues at these different stages, followed by repeat rounds of 5'-RACE (rapid amplification of cDNA ends) and 3'-RACE, led to isolation of a novel gene with an open reading frame encoding a putative protein with 753 amino acids. Its specific expression in the 7-day embryonic retina was confirmed by Northern blot analysis. The gene, named 'retinovin,' would be used as a marker for identifying retinal stem cells present at the early stage of retinal development. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)12-15
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume276
Issue number1
DOIs
Publication statusPublished - Sep 16 2000

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Genes
Retina
Complementary DNA
Polymerase chain reaction
Chick Embryo
Stem cells
Northern Blotting
Open Reading Frames
Neurons
Amplification
Stem Cells
Embryonic Structures
Tissue
Amino Acids
Polymerase Chain Reaction
Gallus gallus retinovin protein
Proteins
Subtractive Hybridization Techniques

Keywords

  • cDNA
  • Chick
  • Development
  • Retina
  • Retinovin
  • Subtractive hybridization

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A novel gene (retinovin) expressed selectively in the early stage of chick retinal development. / Itami, Masako; Matsuo, Toshihiko; Ohtsuki, Hiroshi.

In: Biochemical and Biophysical Research Communications, Vol. 276, No. 1, 16.09.2000, p. 12-15.

Research output: Contribution to journalArticle

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