Abstract
To understand molecular mechanisms underlying the response to pressure in human trabecular cells of the eye, genes induced by cyclic mechanical stretching were isolated by subtractive hybridization assisted by polymerase chain reaction. A novel gene containing an Alu repetitive element in the 5' untranslated region was identified, and its expression was confirmed by Northern blot analysis to be stretch-specific in trabecular cells. The gene was also expressed in the retina, but not in the other tissues, including the brain. The gene encoded a putative small protein with 44 amino acids, which showed homology with neuromedin K. The putative novel protein was named as 'oculomedin,' and would be used as a candidate gene for glaucoma.
| Original language | English |
|---|---|
| Pages (from-to) | 349-351 |
| Number of pages | 3 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 259 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jun 7 1999 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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A novel gene (oculomedin) induced by mechanical stretching in human trabecular cells of the eye. / Sato, Yukiko; Matsuo, Toshihiko; Ohtsuki, Hiroshi.
In: Biochemical and Biophysical Research Communications, Vol. 259, No. 2, 07.06.1999, p. 349-351.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel gene (oculomedin) induced by mechanical stretching in human trabecular cells of the eye
AU - Sato, Yukiko
AU - Matsuo, Toshihiko
AU - Ohtsuki, Hiroshi
PY - 1999/6/7
Y1 - 1999/6/7
N2 - To understand molecular mechanisms underlying the response to pressure in human trabecular cells of the eye, genes induced by cyclic mechanical stretching were isolated by subtractive hybridization assisted by polymerase chain reaction. A novel gene containing an Alu repetitive element in the 5' untranslated region was identified, and its expression was confirmed by Northern blot analysis to be stretch-specific in trabecular cells. The gene was also expressed in the retina, but not in the other tissues, including the brain. The gene encoded a putative small protein with 44 amino acids, which showed homology with neuromedin K. The putative novel protein was named as 'oculomedin,' and would be used as a candidate gene for glaucoma.
AB - To understand molecular mechanisms underlying the response to pressure in human trabecular cells of the eye, genes induced by cyclic mechanical stretching were isolated by subtractive hybridization assisted by polymerase chain reaction. A novel gene containing an Alu repetitive element in the 5' untranslated region was identified, and its expression was confirmed by Northern blot analysis to be stretch-specific in trabecular cells. The gene was also expressed in the retina, but not in the other tissues, including the brain. The gene encoded a putative small protein with 44 amino acids, which showed homology with neuromedin K. The putative novel protein was named as 'oculomedin,' and would be used as a candidate gene for glaucoma.
UR - http://www.scopus.com/inward/record.url?scp=0033532679&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033532679&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1999.0797
DO - 10.1006/bbrc.1999.0797
M3 - Article
C2 - 10362512
AN - SCOPUS:0033532679
VL - 259
SP - 349
EP - 351
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -