A novel gene (oculomedin) induced by mechanical stretching in human trabecular cells of the eye

Yukiko Sato, Toshihiko Matsuo, Hiroshi Ohtsuki

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

To understand molecular mechanisms underlying the response to pressure in human trabecular cells of the eye, genes induced by cyclic mechanical stretching were isolated by subtractive hybridization assisted by polymerase chain reaction. A novel gene containing an Alu repetitive element in the 5' untranslated region was identified, and its expression was confirmed by Northern blot analysis to be stretch-specific in trabecular cells. The gene was also expressed in the retina, but not in the other tissues, including the brain. The gene encoded a putative small protein with 44 amino acids, which showed homology with neuromedin K. The putative novel protein was named as 'oculomedin,' and would be used as a candidate gene for glaucoma.

Original languageEnglish
Pages (from-to)349-351
Number of pages3
JournalBiochemical and Biophysical Research Communications
Volume259
Issue number2
DOIs
Publication statusPublished - Jun 7 1999

Fingerprint

Stretching
Genes
Neurokinin B
Alu Elements
5' Untranslated Regions
Polymerase chain reaction
Northern Blotting
Glaucoma
Retina
Brain
Proteins
Tissue
Amino Acids
Pressure
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A novel gene (oculomedin) induced by mechanical stretching in human trabecular cells of the eye. / Sato, Yukiko; Matsuo, Toshihiko; Ohtsuki, Hiroshi.

In: Biochemical and Biophysical Research Communications, Vol. 259, No. 2, 07.06.1999, p. 349-351.

Research output: Contribution to journalArticle

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