A novel ethanol-hypersensitive mutant of Arabidopsis

Takashi Hirayama, Naoko Fujishige, Takanori Kunii, Noriyuki Nishimura, Satoshi Iuchi, Kazuo Shinozaki

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A novel ethanol-hypersensitive mutant, geko1 (gek1), was isolated from Arabidopsis thaliana. The gek1 mutant displays an enhanced sensitivity (10-100 times greater than the wild type) to ethanol in growth medium, while it grows normally in the absence of ethanol, and responds normally to other alcohols and to environmental stresses such as heat shock and high salinity. The ethanol-hypersensitive phenotype of gek1 requires alcohol dehydrogenase activity, indicating that gek1 is sensitive not to ethanol itself but to the metabolites of ethanol. Consistent with this, gek1 shows enhanced sensitivity to acetaldehyde in the medium. The endogenous acetaldehyde levels were not different between gek1-2 and wild-type seedlings treated with ethanol. These results indicate that the ethanol hypersensitivity of gek1 is due to an enhanced sensitivity to acetaldehyde toxicity, instead of abnormally elevated accumulation of toxic acetaldehyde, which has been thought to be the major cause of ethanol toxicity in mammal cells.

Original languageEnglish
Pages (from-to)703-711
Number of pages9
JournalPlant and Cell Physiology
Volume45
Issue number6
DOIs
Publication statusPublished - Jun 2004
Externally publishedYes

Fingerprint

Arabidopsis
Ethanol
ethanol
mutants
Acetaldehyde
acetaldehyde
toxicity
Alcohol Dehydrogenase
Poisons
alcohol dehydrogenase
Salinity
Seedlings
hypersensitivity
heat stress
Mammals
Shock
Hypersensitivity
alcohols
Arabidopsis thaliana
culture media

Keywords

  • Acetaldehyde
  • Alcohol dehydrogenase
  • Arabidopsis
  • Ethanol-hypersensitive mutant

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Hirayama, T., Fujishige, N., Kunii, T., Nishimura, N., Iuchi, S., & Shinozaki, K. (2004). A novel ethanol-hypersensitive mutant of Arabidopsis. Plant and Cell Physiology, 45(6), 703-711. https://doi.org/10.1093/pcp/pch078

A novel ethanol-hypersensitive mutant of Arabidopsis. / Hirayama, Takashi; Fujishige, Naoko; Kunii, Takanori; Nishimura, Noriyuki; Iuchi, Satoshi; Shinozaki, Kazuo.

In: Plant and Cell Physiology, Vol. 45, No. 6, 06.2004, p. 703-711.

Research output: Contribution to journalArticle

Hirayama, T, Fujishige, N, Kunii, T, Nishimura, N, Iuchi, S & Shinozaki, K 2004, 'A novel ethanol-hypersensitive mutant of Arabidopsis', Plant and Cell Physiology, vol. 45, no. 6, pp. 703-711. https://doi.org/10.1093/pcp/pch078
Hirayama T, Fujishige N, Kunii T, Nishimura N, Iuchi S, Shinozaki K. A novel ethanol-hypersensitive mutant of Arabidopsis. Plant and Cell Physiology. 2004 Jun;45(6):703-711. https://doi.org/10.1093/pcp/pch078
Hirayama, Takashi ; Fujishige, Naoko ; Kunii, Takanori ; Nishimura, Noriyuki ; Iuchi, Satoshi ; Shinozaki, Kazuo. / A novel ethanol-hypersensitive mutant of Arabidopsis. In: Plant and Cell Physiology. 2004 ; Vol. 45, No. 6. pp. 703-711.
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