Abstract
A novel ethanol-hypersensitive mutant, geko1 (gek1), was isolated from Arabidopsis thaliana. The gek1 mutant displays an enhanced sensitivity (10-100 times greater than the wild type) to ethanol in growth medium, while it grows normally in the absence of ethanol, and responds normally to other alcohols and to environmental stresses such as heat shock and high salinity. The ethanol-hypersensitive phenotype of gek1 requires alcohol dehydrogenase activity, indicating that gek1 is sensitive not to ethanol itself but to the metabolites of ethanol. Consistent with this, gek1 shows enhanced sensitivity to acetaldehyde in the medium. The endogenous acetaldehyde levels were not different between gek1-2 and wild-type seedlings treated with ethanol. These results indicate that the ethanol hypersensitivity of gek1 is due to an enhanced sensitivity to acetaldehyde toxicity, instead of abnormally elevated accumulation of toxic acetaldehyde, which has been thought to be the major cause of ethanol toxicity in mammal cells.
| Original language | English |
|---|---|
| Pages (from-to) | 703-711 |
| Number of pages | 9 |
| Journal | Plant and Cell Physiology |
| Volume | 45 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 2004 |
| Externally published | Yes |
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Keywords
- Acetaldehyde
- Alcohol dehydrogenase
- Arabidopsis
- Ethanol-hypersensitive mutant
ASJC Scopus subject areas
- Plant Science
- Physiology
- Cell Biology
Cite this
A novel ethanol-hypersensitive mutant of Arabidopsis. / Hirayama, Takashi; Fujishige, Naoko; Kunii, Takanori; Nishimura, Noriyuki; Iuchi, Satoshi; Shinozaki, Kazuo.
In: Plant and Cell Physiology, Vol. 45, No. 6, 06.2004, p. 703-711.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel ethanol-hypersensitive mutant of Arabidopsis
AU - Hirayama, Takashi
AU - Fujishige, Naoko
AU - Kunii, Takanori
AU - Nishimura, Noriyuki
AU - Iuchi, Satoshi
AU - Shinozaki, Kazuo
PY - 2004/6
Y1 - 2004/6
N2 - A novel ethanol-hypersensitive mutant, geko1 (gek1), was isolated from Arabidopsis thaliana. The gek1 mutant displays an enhanced sensitivity (10-100 times greater than the wild type) to ethanol in growth medium, while it grows normally in the absence of ethanol, and responds normally to other alcohols and to environmental stresses such as heat shock and high salinity. The ethanol-hypersensitive phenotype of gek1 requires alcohol dehydrogenase activity, indicating that gek1 is sensitive not to ethanol itself but to the metabolites of ethanol. Consistent with this, gek1 shows enhanced sensitivity to acetaldehyde in the medium. The endogenous acetaldehyde levels were not different between gek1-2 and wild-type seedlings treated with ethanol. These results indicate that the ethanol hypersensitivity of gek1 is due to an enhanced sensitivity to acetaldehyde toxicity, instead of abnormally elevated accumulation of toxic acetaldehyde, which has been thought to be the major cause of ethanol toxicity in mammal cells.
AB - A novel ethanol-hypersensitive mutant, geko1 (gek1), was isolated from Arabidopsis thaliana. The gek1 mutant displays an enhanced sensitivity (10-100 times greater than the wild type) to ethanol in growth medium, while it grows normally in the absence of ethanol, and responds normally to other alcohols and to environmental stresses such as heat shock and high salinity. The ethanol-hypersensitive phenotype of gek1 requires alcohol dehydrogenase activity, indicating that gek1 is sensitive not to ethanol itself but to the metabolites of ethanol. Consistent with this, gek1 shows enhanced sensitivity to acetaldehyde in the medium. The endogenous acetaldehyde levels were not different between gek1-2 and wild-type seedlings treated with ethanol. These results indicate that the ethanol hypersensitivity of gek1 is due to an enhanced sensitivity to acetaldehyde toxicity, instead of abnormally elevated accumulation of toxic acetaldehyde, which has been thought to be the major cause of ethanol toxicity in mammal cells.
KW - Acetaldehyde
KW - Alcohol dehydrogenase
KW - Arabidopsis
KW - Ethanol-hypersensitive mutant
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UR - http://www.scopus.com/inward/citedby.url?scp=3142613677&partnerID=8YFLogxK
U2 - 10.1093/pcp/pch078
DO - 10.1093/pcp/pch078
M3 - Article
C2 - 15215505
AN - SCOPUS:3142613677
VL - 45
SP - 703
EP - 711
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 6
ER -