TY - JOUR
T1 - A novel ethanol-hypersensitive mutant of Arabidopsis
AU - Hirayama, Takashi
AU - Fujishige, Naoko
AU - Kunii, Takanori
AU - Nishimura, Noriyuki
AU - Iuchi, Satoshi
AU - Shinozaki, Kazuo
N1 - Funding Information:
We thank Drs. M. Minami, T. Mizoguchi, and T. Kuromori for their helpful discussions and suggestions. This work was partly supported by a Grant-in-Aid from the Ministry of Education, Sports, Culture, Science and Technology of Japan, and the RIKEN President’s Special Research Grant to T.H.
PY - 2004/6
Y1 - 2004/6
N2 - A novel ethanol-hypersensitive mutant, geko1 (gek1), was isolated from Arabidopsis thaliana. The gek1 mutant displays an enhanced sensitivity (10-100 times greater than the wild type) to ethanol in growth medium, while it grows normally in the absence of ethanol, and responds normally to other alcohols and to environmental stresses such as heat shock and high salinity. The ethanol-hypersensitive phenotype of gek1 requires alcohol dehydrogenase activity, indicating that gek1 is sensitive not to ethanol itself but to the metabolites of ethanol. Consistent with this, gek1 shows enhanced sensitivity to acetaldehyde in the medium. The endogenous acetaldehyde levels were not different between gek1-2 and wild-type seedlings treated with ethanol. These results indicate that the ethanol hypersensitivity of gek1 is due to an enhanced sensitivity to acetaldehyde toxicity, instead of abnormally elevated accumulation of toxic acetaldehyde, which has been thought to be the major cause of ethanol toxicity in mammal cells.
AB - A novel ethanol-hypersensitive mutant, geko1 (gek1), was isolated from Arabidopsis thaliana. The gek1 mutant displays an enhanced sensitivity (10-100 times greater than the wild type) to ethanol in growth medium, while it grows normally in the absence of ethanol, and responds normally to other alcohols and to environmental stresses such as heat shock and high salinity. The ethanol-hypersensitive phenotype of gek1 requires alcohol dehydrogenase activity, indicating that gek1 is sensitive not to ethanol itself but to the metabolites of ethanol. Consistent with this, gek1 shows enhanced sensitivity to acetaldehyde in the medium. The endogenous acetaldehyde levels were not different between gek1-2 and wild-type seedlings treated with ethanol. These results indicate that the ethanol hypersensitivity of gek1 is due to an enhanced sensitivity to acetaldehyde toxicity, instead of abnormally elevated accumulation of toxic acetaldehyde, which has been thought to be the major cause of ethanol toxicity in mammal cells.
KW - Acetaldehyde
KW - Alcohol dehydrogenase
KW - Arabidopsis
KW - Ethanol-hypersensitive mutant
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U2 - 10.1093/pcp/pch078
DO - 10.1093/pcp/pch078
M3 - Article
C2 - 15215505
AN - SCOPUS:3142613677
VL - 45
SP - 703
EP - 711
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 6
ER -