A novel DNA-binding motif abuts the zinc finger domain of insect nuclear hormone receptor FTZ-F1 and mouse embryonal long terminal repeat-binding protein

Hitoshi Ueda, Guan Cheng Sun, Takehide Murata, Susumu Hirose

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

Fruit fly FTZ-F1, silkworm BmFTZ-F1, and mouse embryonal long terminal repeat-binding protein are members of the nuclear hormone receptor superfamily, which recognizes the same sequence, 5′-PyCAAGG PyCPu-3′. Among these proteins, a 30-amino-acid basic region abutting the C-terminal end of the zinc finger motif, designated the FTZ-F1 box, is conserved. Gel mobility shift competition by various mutant peptides of the DNA-binding region revealed that the FTZ-F1 box as well as the zinc finger motif is involved in the high-affinity binding of FTZ-F1 to its target site. Using a gel mobility shift matrix competition assay, we demonstrated that the FTZ-F1 box governs the recognition of the first three bases, while the zinc finger region recognizes the remaining part of the binding sequence. We also showed that the DNA-binding region of FTZ-F1 recognizes and binds to DNA as a monomer. Occurrence of the FTZ-F1 box sequence in other members of the nuclear hormone receptor superfamily raises the possibility that these receptors constitute a unique subfamily which binds to DNA as a monomer.

Original languageEnglish
Pages (from-to)5667-5672
Number of pages6
JournalMolecular and Cellular Biology
Volume12
Issue number12
Publication statusPublished - Dec 1992
Externally publishedYes

Fingerprint

Insect Hormones
Nucleotide Motifs
Zinc Fingers
Cytoplasmic and Nuclear Receptors
DNA
Gels
Basic Amino Acids
Bombyx
Fruit
Peptides
mouse steroidogenic factor 1
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

A novel DNA-binding motif abuts the zinc finger domain of insect nuclear hormone receptor FTZ-F1 and mouse embryonal long terminal repeat-binding protein. / Ueda, Hitoshi; Sun, Guan Cheng; Murata, Takehide; Hirose, Susumu.

In: Molecular and Cellular Biology, Vol. 12, No. 12, 12.1992, p. 5667-5672.

Research output: Contribution to journalArticle

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