TY - JOUR
T1 - A novel bioassay method for inhibitors of plant transformation
AU - Kanzaki, Hiroshi
AU - Toyoshima, Hiroto
AU - Kobayashi, Akio
AU - Kawazu, Kazuyoshi
N1 - Funding Information:
Acknowledgments. We are grateful to Professors Yasunori Machida and Kenzo Nakamura in the Faculty of Science and the Faculty of Agriculture, respectively, at Nagoya University for providing the A. tumefaciens strains with a intron-GUS binary vector. This work was supported by a Grant-in-Aid to K. Kawazu for Scientific Research (No. 03453145) from the Ministry of Education, Science, and Culture of Japan.
PY - 1994/1
Y1 - 1994/1
N2 - A rapid and quantitative bioassay method for inhibitors of plant transformation by Agrobacterium tumefaciens with an intron-GUS binary vector was devised as an alternative method to the time-consuming potato tuber disk assay. Among several host plants tested, tobacco BY-2 suspension cells were the most preferable for this GUS expression assay. Furthermore, as the phytotoxicity of the test material, if any, is apparent from the growth inhibition of BY-2 suspension cells, this method can exclude phytotoxic compounds.
AB - A rapid and quantitative bioassay method for inhibitors of plant transformation by Agrobacterium tumefaciens with an intron-GUS binary vector was devised as an alternative method to the time-consuming potato tuber disk assay. Among several host plants tested, tobacco BY-2 suspension cells were the most preferable for this GUS expression assay. Furthermore, as the phytotoxicity of the test material, if any, is apparent from the growth inhibition of BY-2 suspension cells, this method can exclude phytotoxic compounds.
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U2 - 10.1080/bbb.58.531
DO - 10.1080/bbb.58.531
M3 - Article
AN - SCOPUS:0028076408
SN - 0916-8451
VL - 58
SP - 531
EP - 534
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 3
ER -