A novel bioassay method for inhibitors of plant transformation

Hiroshi Kanzaki, Hiroto Toyoshima, Akio Kobayashi, Kazuyoshi Kawazu

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Abstract

A rapid and quantitative bioassay method for inhibitors of plant transformation by Agrobacterium tumefaciens with an intron-GUS binary vector was devised as an alternative method to the time-consuming potato tuber disk assay. Among several host plants tested, tobacco BY-2 suspension cells were the most preferable for this GUS expression assay. Furthermore, as the phytotoxicity of the test material, if any, is apparent from the growth inhibition of BY-2 suspension cells, this method can exclude phytotoxic compounds.

Original languageEnglish
Pages (from-to)531-534
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume58
Issue number3
DOIs
Publication statusPublished - 1994

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ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Organic Chemistry
  • Analytical Chemistry
  • Applied Microbiology and Biotechnology
  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)

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