A novel Arabidopsis gene required for ethanol tolerance is conserved among plants and archaea

Naoko Fujishige, Noriyuki Nishimura, Satoshi Iuchi, Takanori Kunii, Kazuo Shinozaki, Takashi Hirayama

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A novel ethanol-hypersensitive mutant, gek1, of Arabidopsis shows 10-100 times greater sensitivity to ethanol compared to the wild type, while it grows normally in the absence of ethanol, and responds normally to other alcohols and to environmental stresses such as heat shock and high salinity. Mapping of the gek1 locus indicated it is a previously unreported locus. In order to address the GEK1 function, we identified the GEK1 gene by means of map-based cloning. The GEK1 gene encodes a novel protein without any known functional motifs. Transgenic Arabidopsis plants overexpressing GEK1 displayed an enhanced tolerance to ethanol and acetaldehyde, suggesting that GEK1 is directly involved in the tolerance to those chemicals. By contrast, expression of GEK1 in E. coli and yeasts did not increase their tolerance to ethanol or acetaldehyde. Interestingly, a similarity search revealed that GEK1-related genes are conserved only in plants and archaea. These results might suggest that plants, and presumably archaea, have a novel mechanism for protection from acetaldehyde toxicity.

Original languageEnglish
Pages (from-to)659-666
Number of pages8
JournalPlant and Cell Physiology
Volume45
Issue number6
DOIs
Publication statusPublished - Jun 2004
Externally publishedYes

Fingerprint

Archaea
Arabidopsis
Ethanol
ethanol
Acetaldehyde
acetaldehyde
Genes
genes
loci
Genetically Modified Plants
Salinity
heat stress
Organism Cloning
molecular cloning
Shock
alcohols
Hot Temperature
Yeasts
Alcohols
genetically modified organisms

Keywords

  • Arabidopsis
  • Archaea
  • Ethanol hypersensitive mutant
  • Evolution
  • Transgenic plant

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

A novel Arabidopsis gene required for ethanol tolerance is conserved among plants and archaea. / Fujishige, Naoko; Nishimura, Noriyuki; Iuchi, Satoshi; Kunii, Takanori; Shinozaki, Kazuo; Hirayama, Takashi.

In: Plant and Cell Physiology, Vol. 45, No. 6, 06.2004, p. 659-666.

Research output: Contribution to journalArticle

Fujishige, Naoko ; Nishimura, Noriyuki ; Iuchi, Satoshi ; Kunii, Takanori ; Shinozaki, Kazuo ; Hirayama, Takashi. / A novel Arabidopsis gene required for ethanol tolerance is conserved among plants and archaea. In: Plant and Cell Physiology. 2004 ; Vol. 45, No. 6. pp. 659-666.
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