A novel aerobactin utilization cluster in Vibrio vulnificus with a gene involved in the transcription regulation of the iutA homologue

Tomotaka Tanabe, Ayaka Naka, Hiroaki Aso, Hiroshi Nakao, Shizuo Narimatsu, Yuji Inoue, Tomomichi Ono, Shigeo Yamamoto

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We demonstrated that Vibrio vulnificus M2799 utilizes aerobactin for growth as an exogenous siderophore under iron-limiting conditions, concomitant with enhanced production of the 76-kDa iron-repressible outer membrane protein. Subsequently, by applying the Fur titration assay method to the M2799 genomic libraries followed by further cloning of the regions surrounding the candidate genes, we identified the 8.4-kb aerobactin utilization gene cluster which consists of five genes arranged in three distinct transcriptional units. It was confirmed by disruption of the corresponding genes that the first unit forming a three-gene operon (vatCDB) and the third unit of a single gene (iutA) encode an ATP-binding cassette transport component and the 76-kDa ferric aerobactin receptor, respectively. The second unit of another single gene (iutR), encodes a homologue of the GntR family of transcriptional repressors. Although transcription of the first and third units was iron-regulated, the iutR gene was transcribed regardless of iron status in the growth medium. Construction of an iutR disruptant coupled with genetic complementation experiments suggested that the gene encodes a transcriptional repressor for iutA. This is the first example of a regulator gene involved in aerobactin-enhanced production of IutA.

Original languageEnglish
Pages (from-to)823-834
Number of pages12
JournalMICROBIOLOGY and IMMUNOLOGY
Volume49
Issue number9
DOIs
Publication statusPublished - Sep 20 2005

Keywords

  • ABC transport system
  • Aerobactin utilization gene cluster
  • Iron-regulated gene
  • Transcription regulation

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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