A novel activating mutation of the K-ras gene in human primary colon adenocarcinoma

Kazunori Tsukuda, Motohiko Tanino, Hiroyuki Soga, Nobuyoshi Shimizu, Kenji Shimizu

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We identified a novel type of point mutation at the 22nd codon of the K-ras gene in a primary colon cancer. The mutation was C to A transversion substituting lysine (AAG) for normal glutamine (CAG) codon. Biological activity of this mutant K-ras gene was tested by expression of full-length cDNA clones in NIH3T3 cells. Most of the K-ras Lys22-transfected cells exhibited an increased saturation density, a lower serum requirement, and transformed morphology reminiscent to the typical K-ras Val12 transformants. However, the tumorigenicity of K-ras Lys22 transformants in nude mice was significantly less potent than that of K-ras Val12 transformants; only a high copy number transformant produced tumors. Even though the activation is incomplete, the finding that the majority of tumor cells in the specimen carried the K-ras Lys22 mutation suggests that this mutation might be advantageous for growth of tumor cells in vivo. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)653-658
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume278
Issue number3
DOIs
Publication statusPublished - Nov 30 2000

Fingerprint

ras Genes
Tumors
Colon
Adenocarcinoma
Genes
Mutation
Cells
Codon
Bioactivity
Glutamine
Neoplasms
Lysine
Complementary DNA
Chemical activation
Point Mutation
Nude Mice
Colonic Neoplasms
Clone Cells
Growth
Serum

Keywords

  • Colon cancer
  • K-ras
  • Mutation
  • Oncogene
  • Signaling
  • Transformation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A novel activating mutation of the K-ras gene in human primary colon adenocarcinoma. / Tsukuda, Kazunori; Tanino, Motohiko; Soga, Hiroyuki; Shimizu, Nobuyoshi; Shimizu, Kenji.

In: Biochemical and Biophysical Research Communications, Vol. 278, No. 3, 30.11.2000, p. 653-658.

Research output: Contribution to journalArticle

Tsukuda, Kazunori ; Tanino, Motohiko ; Soga, Hiroyuki ; Shimizu, Nobuyoshi ; Shimizu, Kenji. / A novel activating mutation of the K-ras gene in human primary colon adenocarcinoma. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 278, No. 3. pp. 653-658.
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