Abstract
Conventional myocardial perfusion PET tracers require onsite tracer production because of their short radioactive half-lives. To investigate the potential of a new 18F-labeled pyridazinone analog ( 18F-BMS-747158-02), we characterized this tracer in a rat model of permanent and transient coronary occlusion using small-animal PET. Methods: Myocardial 18F-BMS-747158-02 distribution in healthy rats (n = 7), rats with transient (3-min) left coronary artery occlusion (n = 11), and rats with permanent left coronary occlusion (n = 11) was analyzed with a dedicated small-animal PET scanner. Results: Normal hearts demonstrated intense and almost homogeneous tracer uptake throughout the left ventricle for more than 2 h. During permanent coronary occlusion, PET demonstrated perfusion defects, which remained unchanged (37.6% ± 8.8%, 37.4% ± 10.2%, and 36.2% ± 9.8% left ventricle at 15, 45, and 115 min, respectively, after tracer injection). After transient ischemia, the induced defect size decreased significantly after reperfusion (16.2% ± 9.3%, 6.0% ± 6.5%, and 1.4% ± 1.3% left ventricle). Tracer reinjection after transient ischemia resulted in normalization of the induced defect. Conclusion: Coronary occlusion yielded distinct myocardial 18F-BMS-747158-02 uptake defects in the area of ischemia, which demonstrated normalization of activity after reperfusion and reinjection. These promising kinetic parameters may allow for assessment of flow using exercise-rest protocols similar to those used in combination with exercise and rest perfusion SPECT.
| Original language | English |
|---|---|
| Pages (from-to) | 1715-1722 |
| Number of pages | 8 |
| Journal | Journal of Nuclear Medicine |
| Volume | 49 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 1 2008 |
| Externally published | Yes |
Fingerprint
Keywords
- F
- Animal imaging
- Cardiology (clinical)
- Infarction
- Ischemia
- Perfusion
- PET
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging
Cite this
A new 18F-labeled myocardial PET tracer : Myocardial uptake after permanent and transient coronary occlusion in rats. / Higuchi, Takahiro; Nekolla, Stephan G.; Huisman, Marc M.; Reder, Sybille; Poethko, Thorsten; Yu, Ming; Wester, Hans Jurgen; Casebier, David S.; Robinson, Simon P.; Botnar, Rene M.; Schwaiger, Markus.
In: Journal of Nuclear Medicine, Vol. 49, No. 10, 01.10.2008, p. 1715-1722.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A new 18F-labeled myocardial PET tracer
T2 - Myocardial uptake after permanent and transient coronary occlusion in rats
AU - Higuchi, Takahiro
AU - Nekolla, Stephan G.
AU - Huisman, Marc M.
AU - Reder, Sybille
AU - Poethko, Thorsten
AU - Yu, Ming
AU - Wester, Hans Jurgen
AU - Casebier, David S.
AU - Robinson, Simon P.
AU - Botnar, Rene M.
AU - Schwaiger, Markus
PY - 2008/10/1
Y1 - 2008/10/1
N2 - Conventional myocardial perfusion PET tracers require onsite tracer production because of their short radioactive half-lives. To investigate the potential of a new 18F-labeled pyridazinone analog ( 18F-BMS-747158-02), we characterized this tracer in a rat model of permanent and transient coronary occlusion using small-animal PET. Methods: Myocardial 18F-BMS-747158-02 distribution in healthy rats (n = 7), rats with transient (3-min) left coronary artery occlusion (n = 11), and rats with permanent left coronary occlusion (n = 11) was analyzed with a dedicated small-animal PET scanner. Results: Normal hearts demonstrated intense and almost homogeneous tracer uptake throughout the left ventricle for more than 2 h. During permanent coronary occlusion, PET demonstrated perfusion defects, which remained unchanged (37.6% ± 8.8%, 37.4% ± 10.2%, and 36.2% ± 9.8% left ventricle at 15, 45, and 115 min, respectively, after tracer injection). After transient ischemia, the induced defect size decreased significantly after reperfusion (16.2% ± 9.3%, 6.0% ± 6.5%, and 1.4% ± 1.3% left ventricle). Tracer reinjection after transient ischemia resulted in normalization of the induced defect. Conclusion: Coronary occlusion yielded distinct myocardial 18F-BMS-747158-02 uptake defects in the area of ischemia, which demonstrated normalization of activity after reperfusion and reinjection. These promising kinetic parameters may allow for assessment of flow using exercise-rest protocols similar to those used in combination with exercise and rest perfusion SPECT.
AB - Conventional myocardial perfusion PET tracers require onsite tracer production because of their short radioactive half-lives. To investigate the potential of a new 18F-labeled pyridazinone analog ( 18F-BMS-747158-02), we characterized this tracer in a rat model of permanent and transient coronary occlusion using small-animal PET. Methods: Myocardial 18F-BMS-747158-02 distribution in healthy rats (n = 7), rats with transient (3-min) left coronary artery occlusion (n = 11), and rats with permanent left coronary occlusion (n = 11) was analyzed with a dedicated small-animal PET scanner. Results: Normal hearts demonstrated intense and almost homogeneous tracer uptake throughout the left ventricle for more than 2 h. During permanent coronary occlusion, PET demonstrated perfusion defects, which remained unchanged (37.6% ± 8.8%, 37.4% ± 10.2%, and 36.2% ± 9.8% left ventricle at 15, 45, and 115 min, respectively, after tracer injection). After transient ischemia, the induced defect size decreased significantly after reperfusion (16.2% ± 9.3%, 6.0% ± 6.5%, and 1.4% ± 1.3% left ventricle). Tracer reinjection after transient ischemia resulted in normalization of the induced defect. Conclusion: Coronary occlusion yielded distinct myocardial 18F-BMS-747158-02 uptake defects in the area of ischemia, which demonstrated normalization of activity after reperfusion and reinjection. These promising kinetic parameters may allow for assessment of flow using exercise-rest protocols similar to those used in combination with exercise and rest perfusion SPECT.
KW - F
KW - Animal imaging
KW - Cardiology (clinical)
KW - Infarction
KW - Ischemia
KW - Perfusion
KW - PET
UR - http://www.scopus.com/inward/record.url?scp=53749103013&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=53749103013&partnerID=8YFLogxK
U2 - 10.2967/jnumed.108.053967
DO - 10.2967/jnumed.108.053967
M3 - Article
C2 - 18794259
AN - SCOPUS:53749103013
VL - 49
SP - 1715
EP - 1722
JO - Journal of Nuclear Medicine
JF - Journal of Nuclear Medicine
SN - 0161-5505
IS - 10
ER -