A new 18F-labeled myocardial PET tracer: Myocardial uptake after permanent and transient coronary occlusion in rats

Takahiro Higuchi, Stephan G. Nekolla, Marc M. Huisman, Sybille Reder, Thorsten Poethko, Ming Yu, Hans Jurgen Wester, David S. Casebier, Simon P. Robinson, Rene M. Botnar, Markus Schwaiger

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Conventional myocardial perfusion PET tracers require onsite tracer production because of their short radioactive half-lives. To investigate the potential of a new 18F-labeled pyridazinone analog ( 18F-BMS-747158-02), we characterized this tracer in a rat model of permanent and transient coronary occlusion using small-animal PET. Methods: Myocardial 18F-BMS-747158-02 distribution in healthy rats (n = 7), rats with transient (3-min) left coronary artery occlusion (n = 11), and rats with permanent left coronary occlusion (n = 11) was analyzed with a dedicated small-animal PET scanner. Results: Normal hearts demonstrated intense and almost homogeneous tracer uptake throughout the left ventricle for more than 2 h. During permanent coronary occlusion, PET demonstrated perfusion defects, which remained unchanged (37.6% ± 8.8%, 37.4% ± 10.2%, and 36.2% ± 9.8% left ventricle at 15, 45, and 115 min, respectively, after tracer injection). After transient ischemia, the induced defect size decreased significantly after reperfusion (16.2% ± 9.3%, 6.0% ± 6.5%, and 1.4% ± 1.3% left ventricle). Tracer reinjection after transient ischemia resulted in normalization of the induced defect. Conclusion: Coronary occlusion yielded distinct myocardial 18F-BMS-747158-02 uptake defects in the area of ischemia, which demonstrated normalization of activity after reperfusion and reinjection. These promising kinetic parameters may allow for assessment of flow using exercise-rest protocols similar to those used in combination with exercise and rest perfusion SPECT.

Original languageEnglish
Pages (from-to)1715-1722
Number of pages8
JournalJournal of Nuclear Medicine
Volume49
Issue number10
DOIs
Publication statusPublished - Oct 1 2008
Externally publishedYes

Fingerprint

Coronary Occlusion
Heart Ventricles
Ischemia
Perfusion
Reperfusion
Single-Photon Emission-Computed Tomography
Coronary Vessels
Injections
BMS 747158-02

Keywords

  • F
  • Animal imaging
  • Cardiology (clinical)
  • Infarction
  • Ischemia
  • Perfusion
  • PET

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

A new 18F-labeled myocardial PET tracer : Myocardial uptake after permanent and transient coronary occlusion in rats. / Higuchi, Takahiro; Nekolla, Stephan G.; Huisman, Marc M.; Reder, Sybille; Poethko, Thorsten; Yu, Ming; Wester, Hans Jurgen; Casebier, David S.; Robinson, Simon P.; Botnar, Rene M.; Schwaiger, Markus.

In: Journal of Nuclear Medicine, Vol. 49, No. 10, 01.10.2008, p. 1715-1722.

Research output: Contribution to journalArticle

Higuchi, T, Nekolla, SG, Huisman, MM, Reder, S, Poethko, T, Yu, M, Wester, HJ, Casebier, DS, Robinson, SP, Botnar, RM & Schwaiger, M 2008, 'A new 18F-labeled myocardial PET tracer: Myocardial uptake after permanent and transient coronary occlusion in rats', Journal of Nuclear Medicine, vol. 49, no. 10, pp. 1715-1722. https://doi.org/10.2967/jnumed.108.053967
Higuchi, Takahiro ; Nekolla, Stephan G. ; Huisman, Marc M. ; Reder, Sybille ; Poethko, Thorsten ; Yu, Ming ; Wester, Hans Jurgen ; Casebier, David S. ; Robinson, Simon P. ; Botnar, Rene M. ; Schwaiger, Markus. / A new 18F-labeled myocardial PET tracer : Myocardial uptake after permanent and transient coronary occlusion in rats. In: Journal of Nuclear Medicine. 2008 ; Vol. 49, No. 10. pp. 1715-1722.
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abstract = "Conventional myocardial perfusion PET tracers require onsite tracer production because of their short radioactive half-lives. To investigate the potential of a new 18F-labeled pyridazinone analog ( 18F-BMS-747158-02), we characterized this tracer in a rat model of permanent and transient coronary occlusion using small-animal PET. Methods: Myocardial 18F-BMS-747158-02 distribution in healthy rats (n = 7), rats with transient (3-min) left coronary artery occlusion (n = 11), and rats with permanent left coronary occlusion (n = 11) was analyzed with a dedicated small-animal PET scanner. Results: Normal hearts demonstrated intense and almost homogeneous tracer uptake throughout the left ventricle for more than 2 h. During permanent coronary occlusion, PET demonstrated perfusion defects, which remained unchanged (37.6{\%} ± 8.8{\%}, 37.4{\%} ± 10.2{\%}, and 36.2{\%} ± 9.8{\%} left ventricle at 15, 45, and 115 min, respectively, after tracer injection). After transient ischemia, the induced defect size decreased significantly after reperfusion (16.2{\%} ± 9.3{\%}, 6.0{\%} ± 6.5{\%}, and 1.4{\%} ± 1.3{\%} left ventricle). Tracer reinjection after transient ischemia resulted in normalization of the induced defect. Conclusion: Coronary occlusion yielded distinct myocardial 18F-BMS-747158-02 uptake defects in the area of ischemia, which demonstrated normalization of activity after reperfusion and reinjection. These promising kinetic parameters may allow for assessment of flow using exercise-rest protocols similar to those used in combination with exercise and rest perfusion SPECT.",
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AU - Higuchi, Takahiro

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AU - Huisman, Marc M.

AU - Reder, Sybille

AU - Poethko, Thorsten

AU - Yu, Ming

AU - Wester, Hans Jurgen

AU - Casebier, David S.

AU - Robinson, Simon P.

AU - Botnar, Rene M.

AU - Schwaiger, Markus

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N2 - Conventional myocardial perfusion PET tracers require onsite tracer production because of their short radioactive half-lives. To investigate the potential of a new 18F-labeled pyridazinone analog ( 18F-BMS-747158-02), we characterized this tracer in a rat model of permanent and transient coronary occlusion using small-animal PET. Methods: Myocardial 18F-BMS-747158-02 distribution in healthy rats (n = 7), rats with transient (3-min) left coronary artery occlusion (n = 11), and rats with permanent left coronary occlusion (n = 11) was analyzed with a dedicated small-animal PET scanner. Results: Normal hearts demonstrated intense and almost homogeneous tracer uptake throughout the left ventricle for more than 2 h. During permanent coronary occlusion, PET demonstrated perfusion defects, which remained unchanged (37.6% ± 8.8%, 37.4% ± 10.2%, and 36.2% ± 9.8% left ventricle at 15, 45, and 115 min, respectively, after tracer injection). After transient ischemia, the induced defect size decreased significantly after reperfusion (16.2% ± 9.3%, 6.0% ± 6.5%, and 1.4% ± 1.3% left ventricle). Tracer reinjection after transient ischemia resulted in normalization of the induced defect. Conclusion: Coronary occlusion yielded distinct myocardial 18F-BMS-747158-02 uptake defects in the area of ischemia, which demonstrated normalization of activity after reperfusion and reinjection. These promising kinetic parameters may allow for assessment of flow using exercise-rest protocols similar to those used in combination with exercise and rest perfusion SPECT.

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