A new risk assessment method for oxidative chemicals using catalase mutant escherichia coli strains

Dahong Wang, Masako Horita, Ken Tsutsui, Kimiko Tsutsui, Kuniaki Sano, Noriyoshi Masuoka, Kenichi Tomochika, Shohei Kira

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We examined the possibility of developing a risk assessment method for potentially hazardous chemicals by using catalase mutant strains. These strains were constructed by transforming plasmids encoding the catalase cDNA from catalase mutant mice (Csb, Csc) and its corresponding wild type (Csa) into catalase-deficient Escherichia coli UM255. Their catalase activities were in the following orders: Csa>Cs c>Csb>UMZSS, whereas their susceptibilities to H2O2 were UM255>Csb>Cs c>Csa. Chemical-induced cytotoxicities, such as hydroquinone and some inorganic heavy metals, were examined by a growth inhibition assay. The inhibition for bacterial growth was determined by measuring the diameter of the inhibitory zone. We found that the susceptibilities of UM255, Csa, Csb and Csc strains to various chemicals negatively correlated with their catalase levels, suggesting the involvement of H2O2 in the processes of chemical- induced cytotoxicity. The results support the usefulness of these newly established strains for hazard identification of oxidative chemicals in a risk assessment process.

Original languageEnglish
Title of host publicationREWAS'04 - Global Symposium on Recycling, Waste Treatment and Clean Technology
EditorsI. Gaballah, B. Mishra, R. Solozabal, M. Tanaka
Pages1405-1413
Number of pages9
Publication statusPublished - 2005
EventREWAS'04 - Global Symposium on Recycling, Waste Treatment and Clean Technology - Madrid, Spain
Duration: Sep 26 2004Sep 29 2004

Other

OtherREWAS'04 - Global Symposium on Recycling, Waste Treatment and Clean Technology
CountrySpain
CityMadrid
Period9/26/049/29/04

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Risk assessment
Escherichia coli
Cytotoxicity
Heavy metals
Assays
Hazards

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Wang, D., Horita, M., Tsutsui, K., Tsutsui, K., Sano, K., Masuoka, N., ... Kira, S. (2005). A new risk assessment method for oxidative chemicals using catalase mutant escherichia coli strains. In I. Gaballah, B. Mishra, R. Solozabal, & M. Tanaka (Eds.), REWAS'04 - Global Symposium on Recycling, Waste Treatment and Clean Technology (pp. 1405-1413)

A new risk assessment method for oxidative chemicals using catalase mutant escherichia coli strains. / Wang, Dahong; Horita, Masako; Tsutsui, Ken; Tsutsui, Kimiko; Sano, Kuniaki; Masuoka, Noriyoshi; Tomochika, Kenichi; Kira, Shohei.

REWAS'04 - Global Symposium on Recycling, Waste Treatment and Clean Technology. ed. / I. Gaballah; B. Mishra; R. Solozabal; M. Tanaka. 2005. p. 1405-1413.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wang, D, Horita, M, Tsutsui, K, Tsutsui, K, Sano, K, Masuoka, N, Tomochika, K & Kira, S 2005, A new risk assessment method for oxidative chemicals using catalase mutant escherichia coli strains. in I Gaballah, B Mishra, R Solozabal & M Tanaka (eds), REWAS'04 - Global Symposium on Recycling, Waste Treatment and Clean Technology. pp. 1405-1413, REWAS'04 - Global Symposium on Recycling, Waste Treatment and Clean Technology, Madrid, Spain, 9/26/04.
Wang D, Horita M, Tsutsui K, Tsutsui K, Sano K, Masuoka N et al. A new risk assessment method for oxidative chemicals using catalase mutant escherichia coli strains. In Gaballah I, Mishra B, Solozabal R, Tanaka M, editors, REWAS'04 - Global Symposium on Recycling, Waste Treatment and Clean Technology. 2005. p. 1405-1413
Wang, Dahong ; Horita, Masako ; Tsutsui, Ken ; Tsutsui, Kimiko ; Sano, Kuniaki ; Masuoka, Noriyoshi ; Tomochika, Kenichi ; Kira, Shohei. / A new risk assessment method for oxidative chemicals using catalase mutant escherichia coli strains. REWAS'04 - Global Symposium on Recycling, Waste Treatment and Clean Technology. editor / I. Gaballah ; B. Mishra ; R. Solozabal ; M. Tanaka. 2005. pp. 1405-1413
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