TY - GEN
T1 - A new risk assessment method for oxidative chemicals using catalase mutant escherichia coli strains
AU - Wang, Dahong
AU - Horita, Masako
AU - Tsutsui, Ken
AU - Tsutsui, Kimiko
AU - Sano, Kuniaki
AU - Masuoka, Noriyoshi
AU - Tomochika, Kenichi
AU - Kira, Shohei
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005
Y1 - 2005
N2 - We examined the possibility of developing a risk assessment method for potentially hazardous chemicals by using catalase mutant strains. These strains were constructed by transforming plasmids encoding the catalase cDNA from catalase mutant mice (Csb, Csc) and its corresponding wild type (Csa) into catalase-deficient Escherichia coli UM255. Their catalase activities were in the following orders: Csa>Cs c>Csb>UMZSS, whereas their susceptibilities to H2O2 were UM255>Csb>Cs c>Csa. Chemical-induced cytotoxicities, such as hydroquinone and some inorganic heavy metals, were examined by a growth inhibition assay. The inhibition for bacterial growth was determined by measuring the diameter of the inhibitory zone. We found that the susceptibilities of UM255, Csa, Csb and Csc strains to various chemicals negatively correlated with their catalase levels, suggesting the involvement of H2O2 in the processes of chemical- induced cytotoxicity. The results support the usefulness of these newly established strains for hazard identification of oxidative chemicals in a risk assessment process.
AB - We examined the possibility of developing a risk assessment method for potentially hazardous chemicals by using catalase mutant strains. These strains were constructed by transforming plasmids encoding the catalase cDNA from catalase mutant mice (Csb, Csc) and its corresponding wild type (Csa) into catalase-deficient Escherichia coli UM255. Their catalase activities were in the following orders: Csa>Cs c>Csb>UMZSS, whereas their susceptibilities to H2O2 were UM255>Csb>Cs c>Csa. Chemical-induced cytotoxicities, such as hydroquinone and some inorganic heavy metals, were examined by a growth inhibition assay. The inhibition for bacterial growth was determined by measuring the diameter of the inhibitory zone. We found that the susceptibilities of UM255, Csa, Csb and Csc strains to various chemicals negatively correlated with their catalase levels, suggesting the involvement of H2O2 in the processes of chemical- induced cytotoxicity. The results support the usefulness of these newly established strains for hazard identification of oxidative chemicals in a risk assessment process.
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M3 - Conference contribution
AN - SCOPUS:32044454029
SN - 8495520052
T3 - REWAS'04 - Global Symposium on Recycling, Waste Treatment and Clean Technology
SP - 1405
EP - 1413
BT - REWAS'04 - Global Symposium on Recycling, Waste Treatment and Clean Technology - Proceedings
A2 - Gaballah, I.
A2 - Mishra, B.
A2 - Solozabal, R.
A2 - Tanaka, M.
T2 - REWAS'04 - Global Symposium on Recycling, Waste Treatment and Clean Technology
Y2 - 26 September 2004 through 29 September 2004
ER -
