A new resistance gene in combination with Rmg8 confers strong resistance against triticum isolates of Pyricularia oryzae in a common wheat landrace

Shizhen Wang, Soichiro Asuke, Trinh Thi Phuong Vy, Yoshihiro Inoue, Izumi Chuma, Joe Win, Kenji Kato, Yukio Tosa

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Abstract

The wheat blast fungus (Triticum pathotype of Pyricularia oryzae) first arose in Brazil in 1985 and has recently spread to Asia. Resistance genes against this new pathogen are very rare in common wheat populations. We screened 520 local landraces of common wheat collected worldwide with Br48, a Triticum isolate collected in Brazil, and found a highly resistant, unique accession, GR119. When F2 seedlings derived from a cross between GR119 and Chinese Spring (CS, susceptible control) were inoculated with Br48, resistant and susceptible seedlings segregated in a 15:1 ratio, suggesting that GR119 carries two resistance genes. When the F2 seedlings were inoculated with Br48DA8 carrying a disrupted allele of AVR-Rmg8 (an avirulence gene corresponding to a previously reported resistance gene, Rmg8), however, the segregation fitted a 3:1 ratio. These results suggest that one of the two genes in GR119 was Rmg8. The other, new gene was tentatively designated as RmgGR119. GR119 was highly resistant to all Triticum isolates tested. Spikes of GR119 were highly resistant to Br48, moderately resistant to Br48DA8 and a hybrid culture carrying avr-Rmg8 (nonfunctional allele), and highly resistant to its transformant carrying AVR-Rmg8. The strong resistance of GR119 was attributed to the combined effects of Rmg8 and RmgGR119.

Original languageEnglish
Pages (from-to)1299-1306
Number of pages8
JournalPhytopathology
Volume108
Issue number11
DOIs
Publication statusPublished - Nov 1 2018

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Magnaporthe oryzae
Triticum
landraces
wheat
genes
seedlings
alleles
Brazil
pathotypes
inflorescences
fungi
pathogens

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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A new resistance gene in combination with Rmg8 confers strong resistance against triticum isolates of Pyricularia oryzae in a common wheat landrace. / Wang, Shizhen; Asuke, Soichiro; Vy, Trinh Thi Phuong; Inoue, Yoshihiro; Chuma, Izumi; Win, Joe; Kato, Kenji; Tosa, Yukio.

In: Phytopathology, Vol. 108, No. 11, 01.11.2018, p. 1299-1306.

Research output: Contribution to journalArticle

Wang, Shizhen ; Asuke, Soichiro ; Vy, Trinh Thi Phuong ; Inoue, Yoshihiro ; Chuma, Izumi ; Win, Joe ; Kato, Kenji ; Tosa, Yukio. / A new resistance gene in combination with Rmg8 confers strong resistance against triticum isolates of Pyricularia oryzae in a common wheat landrace. In: Phytopathology. 2018 ; Vol. 108, No. 11. pp. 1299-1306.
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