A new planar-type leakage current and impedance microsensor for detection of interaction between electrolyte-entrapping liposome and protein

P. Lorchirachoonkul, I. Goto, Toshinori Shimanouchi, K. Yamashita, M. Noda

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed a new leakage current microsensor by using simpler planar processes than Si-surface-bulk micromachining processes used in the previous microwell structure. This sensor fabrication and structure can easily make a target solution volume smaller than μL with excellent immobilization of the droplet and intact biomolecules as sensing elements, as a result, reduce effectively the background noise current in the microsensor and improve reproducibility of the results. The leakage current due to the biochemical interaction was successfully evaluated, dependent on the droplet protein concentration. Cole-Cole plots from the impedance analysis also show quantitative difference between with and without the interaction, depending on the charge-transfer impedance that results from the condition and structure of liposome and lipid membrane after the interaction.

Original languageEnglish
Title of host publicationProcedia Engineering
Pages1449-1452
Number of pages4
Volume25
DOIs
Publication statusPublished - 2011
Externally publishedYes
Event25th Eurosensors Conference - Athens, Greece
Duration: Sep 4 2011Sep 7 2011

Other

Other25th Eurosensors Conference
CountryGreece
CityAthens
Period9/4/119/7/11

Fingerprint

Microsensors
Liposomes
Leakage currents
Electrolytes
Proteins
Micromachining
Biomolecules
Charge transfer
Fabrication
Sensors
Membrane Lipids

Keywords

  • Electrochemical
  • Impedance
  • Leakage current
  • Liposome
  • Microsensor
  • Protein

ASJC Scopus subject areas

  • Engineering(all)

Cite this

A new planar-type leakage current and impedance microsensor for detection of interaction between electrolyte-entrapping liposome and protein. / Lorchirachoonkul, P.; Goto, I.; Shimanouchi, Toshinori; Yamashita, K.; Noda, M.

Procedia Engineering. Vol. 25 2011. p. 1449-1452.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lorchirachoonkul, P, Goto, I, Shimanouchi, T, Yamashita, K & Noda, M 2011, A new planar-type leakage current and impedance microsensor for detection of interaction between electrolyte-entrapping liposome and protein. in Procedia Engineering. vol. 25, pp. 1449-1452, 25th Eurosensors Conference, Athens, Greece, 9/4/11. https://doi.org/10.1016/j.proeng.2011.12.358
Lorchirachoonkul, P. ; Goto, I. ; Shimanouchi, Toshinori ; Yamashita, K. ; Noda, M. / A new planar-type leakage current and impedance microsensor for detection of interaction between electrolyte-entrapping liposome and protein. Procedia Engineering. Vol. 25 2011. pp. 1449-1452
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AU - Yamashita, K.

AU - Noda, M.

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AB - We have developed a new leakage current microsensor by using simpler planar processes than Si-surface-bulk micromachining processes used in the previous microwell structure. This sensor fabrication and structure can easily make a target solution volume smaller than μL with excellent immobilization of the droplet and intact biomolecules as sensing elements, as a result, reduce effectively the background noise current in the microsensor and improve reproducibility of the results. The leakage current due to the biochemical interaction was successfully evaluated, dependent on the droplet protein concentration. Cole-Cole plots from the impedance analysis also show quantitative difference between with and without the interaction, depending on the charge-transfer impedance that results from the condition and structure of liposome and lipid membrane after the interaction.

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