A new mitochondrial DNA deletion associated with diabetic amyotrophy, diabetic myoatrophy and diabetic fatty liver

Yoshinori Hinokio, Susumu Suzuki, Koga Komatu, Masataka Ohtomo, Masatoshi Onoda, Masahiro Matsumoto, Satoshi Hirali, Yoshinori Sato, Hiroaki Akai, Koji Abe, Shigeaki Miyabayasi, Ryuzo Abe, Takayoshi Toyota

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Mitochondrial dysfunctions of the muscle in diabetic amyotrophy and of the liver in diabetic fatty liver have been reported. We investigated mitochondrial gene mutations in three cases: (1) a patient with diabetic amyotrophy in the muscles of the lower extremities, and neuropathy; (2) 5 diabetics with myoatrophy, diabetic nephropathy, and chronic renal failure; and (3) an IDDM patient with a diabetic fatty liver. We identified a 5778‐bp deletion (8214–13991) in mitochondrial DNA from the muscle and liver biopsy specimens by the primer shift PCR and PCR‐direct sequence methods. It is speculated that 5778‐bp deletion is due to homogeneous recombination in the 7‐bp repeat sequence of TCCTAGA flanking the region deleted in the mitochondrial DNA. Determination of respiratory chain enzyme activities in fresh muscle mitochondria demonstrated the defect in complex I activity. The deletion covers areas coding ND3, ND4, ND4L, and ND5 in complex I. The 5778‐bp deletion might cause a defect in mitochondrial oxidative phosphorylation and contribute to the pathogenesis of diabetic amyotrophy, myoatrophy with diabetic nephropathy, and chronic renal failure, as well as diabetic fatty liver in IDDM. © 1995 John Wiley & Sons, Inc.

Original languageEnglish
Pages (from-to)S142-S149
JournalMuscle & Nerve
Volume18
Issue number14 S
DOIs
Publication statusPublished - 1995
Externally publishedYes

Keywords

  • chronic renal failure
  • deletion
  • diabetic amyotrophy
  • diabetic fatty liver
  • mitochondrial DNA

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)

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  • Cite this

    Hinokio, Y., Suzuki, S., Komatu, K., Ohtomo, M., Onoda, M., Matsumoto, M., Hirali, S., Sato, Y., Akai, H., Abe, K., Miyabayasi, S., Abe, R., & Toyota, T. (1995). A new mitochondrial DNA deletion associated with diabetic amyotrophy, diabetic myoatrophy and diabetic fatty liver. Muscle & Nerve, 18(14 S), S142-S149. https://doi.org/10.1002/mus.880181428