A new method for sex determination based on detection of SRY, STS and amelogenin gene regions with simultaneous amplification of their homologous sequences by a multiplex PCR

Toshio Morikawaa, Yuji Yamamoto, Satoru Miyaish

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have developed a new method for sex determination based on simultaneous detection of the SRY (sex-determining region Y), STS (steroid sulfatase) and amelogenin (AMELX and AMELY) gene regions and their homologous sequences. The sex of 246 blood samples was correctly determined by this method. An AMELY-deleted male sample, which would have been erroneously considered female based solely on analysis of the amelogenin locus, was successfully identified as male by the present method. The detection limit of this method was 63 pg of genomic DNA, and the male DNA component could be detected from mixed samples having a male : female ratio as low as 1:10. This method was useful for degraded DNA and possessed the human specificity. Practical application to 35 autopsy cases is described.

Original languageEnglish
Pages (from-to)113-122
Number of pages10
JournalActa medica Okayama
Volume65
Issue number2
Publication statusPublished - May 12 2011

Keywords

  • Forensic casework
  • Multiplex PCR
  • SRY (sex-determining region Y)
  • Sex determination

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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