A new method for improving the enantioselectivity of lipase-catalyzed hydrolysis in organic solvent containing a small amount of water in the presence of metal ions

Takashi Okamoto, Shinichi Ueji

Research output: Contribution to journalArticle

13 Citations (Scopus)


The hydrolysis of racemic butyl 2-(4-substituted phenoxy)propionates having various substituents catalyzed by lipase MY from Candida rugosa was achieved in di-isopropyl ether containing 0.75% (v/v) of 2.4 M LiCl or 1.2 M MgCl2 aqueous solution. Water molecules hydrated to the metal ion in isopropyl ether acted as a nucleophile to cause the hydrolysis of these esters as with water alone. Metal ions used significantly enhanced their enantioselectivities by 100-fold or above, as compared with the ordinary reaction media.

Original languageEnglish
Pages (from-to)1169-1171
Number of pages3
JournalBiotechnology Letters
Issue number14
Publication statusPublished - Sep 19 2000



  • Enantioselectivity
  • Hydrolysis
  • Lipase
  • Metal ion

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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