Abstract
The hydrolysis of racemic butyl 2-(4-substituted phenoxy)propionates having various substituents catalyzed by lipase MY from Candida rugosa was achieved in di-isopropyl ether containing 0.75% (v/v) of 2.4 M LiCl or 1.2 M MgCl2 aqueous solution. Water molecules hydrated to the metal ion in isopropyl ether acted as a nucleophile to cause the hydrolysis of these esters as with water alone. Metal ions used significantly enhanced their enantioselectivities by 100-fold or above, as compared with the ordinary reaction media.
| Original language | English |
|---|---|
| Pages (from-to) | 1169-1171 |
| Number of pages | 3 |
| Journal | Biotechnology Letters |
| Volume | 22 |
| Issue number | 14 |
| DOIs | |
| Publication status | Published - 2000 |
| Externally published | Yes |
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Keywords
- Enantioselectivity
- Hydrolysis
- Lipase
- Metal ion
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
- Microbiology
- Bioengineering
Cite this
A new method for improving the enantioselectivity of lipase-catalyzed hydrolysis in organic solvent containing a small amount of water in the presence of metal ions. / Okamoto, Takashi; Ueji, Shinichi.
In: Biotechnology Letters, Vol. 22, No. 14, 2000, p. 1169-1171.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A new method for improving the enantioselectivity of lipase-catalyzed hydrolysis in organic solvent containing a small amount of water in the presence of metal ions
AU - Okamoto, Takashi
AU - Ueji, Shinichi
PY - 2000
Y1 - 2000
N2 - The hydrolysis of racemic butyl 2-(4-substituted phenoxy)propionates having various substituents catalyzed by lipase MY from Candida rugosa was achieved in di-isopropyl ether containing 0.75% (v/v) of 2.4 M LiCl or 1.2 M MgCl2 aqueous solution. Water molecules hydrated to the metal ion in isopropyl ether acted as a nucleophile to cause the hydrolysis of these esters as with water alone. Metal ions used significantly enhanced their enantioselectivities by 100-fold or above, as compared with the ordinary reaction media.
AB - The hydrolysis of racemic butyl 2-(4-substituted phenoxy)propionates having various substituents catalyzed by lipase MY from Candida rugosa was achieved in di-isopropyl ether containing 0.75% (v/v) of 2.4 M LiCl or 1.2 M MgCl2 aqueous solution. Water molecules hydrated to the metal ion in isopropyl ether acted as a nucleophile to cause the hydrolysis of these esters as with water alone. Metal ions used significantly enhanced their enantioselectivities by 100-fold or above, as compared with the ordinary reaction media.
KW - Enantioselectivity
KW - Hydrolysis
KW - Lipase
KW - Metal ion
UR - http://www.scopus.com/inward/record.url?scp=0033865694&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033865694&partnerID=8YFLogxK
U2 - 10.1023/A:1005637329704
DO - 10.1023/A:1005637329704
M3 - Article
AN - SCOPUS:0033865694
VL - 22
SP - 1169
EP - 1171
JO - Biotechnology Letters
JF - Biotechnology Letters
SN - 0141-5492
IS - 14
ER -