A new iron oxidase from a moderately thermophilic iron oxidizing bacterium strain TI-1

Masaki Takai, Kazuo Kamimura, Tsuyoshi Sugio

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Iron oxidase was purified from plasma membranes of a moderately thermophilic iron oxidizing bacterium strain TI-1 in an electrophoretically homogenous state. Spectrum analyses of purified enzyme showed the existence of cytochrome a, but not cytochrome b and c types. Iron oxidase was composed of five subunits with apparent molecular masses of 46 kDa (α), 28 kDa (β), 24 kDa (γ), 20 kDa (δ), and 17 kDa (ε). As the molecular mass of a native enzyme was estimated to be 263 kDa in the presence of 0.1% n-dodecyl-β-D-maltopyranoside (DM), a native iron oxidase purified from strain TI-1 seems to be a homodimeric enzyme (αβγδε)2. Optimum pH and temperature for iron oxidation were pH 3.0 and 45°C, respectively. The Km of iron oxidase for Fe2+ was 1.06 mM and Vmax for O2 uptake was 13.8 μmol·mg-1·min-1. The activity was strongly inhibited by cyanide and azide. Purified enzyme from strain TI-1 is a new iron oxidase in which electrons of Fe2+ were transferred to haem a and then to the molecular oxygen.

Original languageEnglish
Pages (from-to)1653-1658
Number of pages6
JournalEuropean Journal of Biochemistry
Volume268
Issue number6
DOIs
Publication statusPublished - 2001

Fingerprint

Bacteria
Oxidoreductases
Iron
Molecular mass
Enzymes
Cytochromes a
Cytochromes b
Azides
Molecular oxygen
Cyanides
Cell membranes
Cytochromes c
Heme
Spectrum Analysis
Cell Membrane
Electrons
Oxygen
Oxidation
Temperature

Keywords

  • Cytochrome a
  • Iron oxidase
  • Iron oxidizing bacterium
  • Moderate thermophile
  • Thermophile

ASJC Scopus subject areas

  • Biochemistry

Cite this

A new iron oxidase from a moderately thermophilic iron oxidizing bacterium strain TI-1. / Takai, Masaki; Kamimura, Kazuo; Sugio, Tsuyoshi.

In: European Journal of Biochemistry, Vol. 268, No. 6, 2001, p. 1653-1658.

Research output: Contribution to journalArticle

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