A new experimental system for irradiating tumors in mice using a linear accelerator under specific pathogen-free conditions

Masahiro Kuroda, Keiji Inamura, Seiji Tahara, Yuzuru Kurabayashi, Tadaatsu Akagi, Jun-Ichi Asaumi, Izumi Togami, Mitsuhiro Takemoto, Osamu Honda, Yasuki Morioka, Shoji Kawasaki, Yoshio Hirak

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We developed a reliable system for the irradiation of xenografted tumors in mice which allows for accurate local irradiation under specific pathogen-free conditions. The system presented here consists of acrylic supports for mice and an acrylic box connected to a pump through 0.22 μm pore-sized filters. Mice with xenotransplanted tumors growing on their right hind legs were set on the supports and put into the box in a laminar flow hood. The tumors of 7 mice were irradiated simultaneously with X-rays of 6 and 10 MV generated by a linear accelerator at a dose rate of 3.1-4.7 Gy/min. The air was ventilated through filters during irradiation in the closed box. Microorganism tests confirmed that no bacteria entered or left the box. One of the significant characteristics of this setup is that it allows for irradiation under conditions of acute hypoxia, which is obtained using an integrated tourniquet. The dose variation among 7 tumors was less than 1%. The rest of the mouse's body was shielded effectively by a half-field technique and a lead block. As a result, the whole body dose for the mice was 0-4% of the total dose absorbed by the tumor. Due to the high dose rate and the ability to irradiate 7 mice simultaneously under specific pathogen-free conditions, this new system can be considered a time-saving and valuable tool for radiation oncology research.

Original languageEnglish
Pages (from-to)111-118
Number of pages8
JournalActa Medica Okayama
Volume53
Issue number3
Publication statusPublished - 1999

Fingerprint

Specific Pathogen-Free Organisms
Particle Accelerators
Linear accelerators
Pathogens
Tumors
Irradiation
Neoplasms
Acrylics
Oncology
Laminar flow
Microorganisms
Tourniquets
Radiation Oncology
Bacteria
Pumps
Radiation
X rays
Leg
Air
X-Rays

Keywords

  • Animal experiment
  • Linear accelerator
  • Mouse
  • Radiotherapy
  • Specific pathogen-free

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

A new experimental system for irradiating tumors in mice using a linear accelerator under specific pathogen-free conditions. / Kuroda, Masahiro; Inamura, Keiji; Tahara, Seiji; Kurabayashi, Yuzuru; Akagi, Tadaatsu; Asaumi, Jun-Ichi; Togami, Izumi; Takemoto, Mitsuhiro; Honda, Osamu; Morioka, Yasuki; Kawasaki, Shoji; Hirak, Yoshio.

In: Acta Medica Okayama, Vol. 53, No. 3, 1999, p. 111-118.

Research output: Contribution to journalArticle

Kuroda, M, Inamura, K, Tahara, S, Kurabayashi, Y, Akagi, T, Asaumi, J-I, Togami, I, Takemoto, M, Honda, O, Morioka, Y, Kawasaki, S & Hirak, Y 1999, 'A new experimental system for irradiating tumors in mice using a linear accelerator under specific pathogen-free conditions', Acta Medica Okayama, vol. 53, no. 3, pp. 111-118.
Kuroda, Masahiro ; Inamura, Keiji ; Tahara, Seiji ; Kurabayashi, Yuzuru ; Akagi, Tadaatsu ; Asaumi, Jun-Ichi ; Togami, Izumi ; Takemoto, Mitsuhiro ; Honda, Osamu ; Morioka, Yasuki ; Kawasaki, Shoji ; Hirak, Yoshio. / A new experimental system for irradiating tumors in mice using a linear accelerator under specific pathogen-free conditions. In: Acta Medica Okayama. 1999 ; Vol. 53, No. 3. pp. 111-118.
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