A new ether bond-splitting enzyme found in Gram-positive polyethylene glycol 6000-utilizing bacterium, Pseudonocardia sp. strain K1

Manabu Yamashita, Akiwo Tani, Fusako Kawai

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Pseudonocardia sp. strain K1 is the only Gram-positive bacterium among the bacteria aerobically metabolizing polyethylene glycol (PEG). Generally, PEG is metabolized by an oxidative pathway in which a terminal alcohol group of PEG is oxidized to aldehyde and to carboxylic acid and then an ether bond is oxidatively cleaved. As the cell-free extract of Pseudonocardia sp. strain K1 has PEG dehydrogenase, PEG aldehyde dehydrogenase and diglycolic acid (DGA) dehydrogenase (DGADH) activities, all of which are constitutively formed, the strain has a metabolic pathway similar to that so far known. We purified an ether bond-splitting enzyme as DGADH. The molecular mass of the enzyme was estimated to be 55 kDa; and it consisted of two identical subunits. The enzyme oxidatively cleaved both an ether bond of PEG 3000 dicarboxylic acid and DGA. The N-terminal amino acid sequence of the purified enzyme had high homology with various superoxide dismutases and the enzyme had also superoxide dismutase activity. The atomic absorption spectrum showed that approximately one atom of Fe was included in each subunit of the enzyme. DGADH activity increased in the cells grown in a PEG medium supplemented with FeCl3. Thus, we concluded that the enzyme purified from Pseudonocardia sp. strain K1 is a new ether bond-splitting enzyme.

Original languageEnglish
Pages (from-to)174-179
Number of pages6
JournalApplied Microbiology and Biotechnology
Volume66
Issue number2
DOIs
Publication statusPublished - Dec 2004

Fingerprint

Ether
Polyethylene glycols
Ethers
Bacteria
Enzymes
Oxidoreductases
Glycolaldehyde Dehydrogenase
Aldehydes
Superoxide Dismutase
Acids
Dicarboxylic Acids
Polyethylene Glycol 6000
Molecular mass
Gram-Positive Bacteria
Carboxylic Acids
Metabolic Networks and Pathways
Cell Extracts
Carboxylic acids
Amino acids
Absorption spectra

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Bioengineering
  • Microbiology (medical)

Cite this

A new ether bond-splitting enzyme found in Gram-positive polyethylene glycol 6000-utilizing bacterium, Pseudonocardia sp. strain K1. / Yamashita, Manabu; Tani, Akiwo; Kawai, Fusako.

In: Applied Microbiology and Biotechnology, Vol. 66, No. 2, 12.2004, p. 174-179.

Research output: Contribution to journalArticle

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