Abstract
This paper reports a new type of detection approach by arrayed biosensor for discrimination of target biomolecules such as protein and/or evaluation of their concentrations, utilizing liposome encapsulating fluorescent molecules and the corresponding time course analysis of the fluorescence. This method is realized by obtaining multi-dimensional data plots (curve) of fluorescent output such as its intensity from plural and different liposomes independent from each other. Two different proteins (carbonic anhydrase from bovine: CAB and Lysozyme), including their concentrations, were clearly discriminated by the developed method.
| Original language | English |
|---|---|
| Title of host publication | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 194-196 |
| Number of pages | 3 |
| ISBN (Print) | 9781632666246 |
| Publication status | Published - Jan 1 2013 |
| Event | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany Duration: Oct 27 2013 → Oct 31 2013 |
Publication series
| Name | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
|---|---|
| Volume | 1 |
Other
| Other | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
|---|---|
| Country | Germany |
| City | Freiburg |
| Period | 10/27/13 → 10/31/13 |
Keywords
- Discrimination method
- Fluorescence
- Liposome
- Protein
- Sensor array
- Target biomolecule
ASJC Scopus subject areas
- Bioengineering
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Cite this
Takada, K., Fujimoto, T., Shimanouchi, T., Fukuzawa, M., Yamashita, K., Umakoshi, H., & Noda, M. (2013). A new discrimination method of target biomolecules with miniaturized sensor array utilizing liposome encapsulating fluorescent molecules with time course analysis. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 194-196). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1). Chemical and Biological Microsystems Society.