Abstract
This paper reports a new type of detection approach by arrayed biosensor for discrimination of target biomolecules such as protein and/or evaluation of their concentrations, utilizing liposome encapsulating fluorescent molecules and the corresponding time course analysis of the fluorescence. This method is realized by obtaining multi-dimensional data plots (curve) of fluorescent output such as its intensity from plural and different liposomes independent from each other. Two different proteins (carbonic anhydrase from bovine: CAB and Lysozyme), including their concentrations, were clearly discriminated by the developed method.
| Original language | English |
|---|---|
| Title of host publication | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 194-196 |
| Number of pages | 3 |
| Volume | 1 |
| ISBN (Print) | 9781632666246 |
| Publication status | Published - 2013 |
| Event | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany Duration: Oct 27 2013 → Oct 31 2013 |
Other
| Other | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
|---|---|
| Country | Germany |
| City | Freiburg |
| Period | 10/27/13 → 10/31/13 |
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Keywords
- Discrimination method
- Fluorescence
- Liposome
- Protein
- Sensor array
- Target biomolecule
ASJC Scopus subject areas
- Bioengineering
Cite this
A new discrimination method of target biomolecules with miniaturized sensor array utilizing liposome encapsulating fluorescent molecules with time course analysis. / Takada, K.; Fujimoto, T.; Shimanouchi, Toshinori; Fukuzawa, M.; Yamashita, K.; Umakoshi, H.; Noda, M.
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1 Chemical and Biological Microsystems Society, 2013. p. 194-196.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - A new discrimination method of target biomolecules with miniaturized sensor array utilizing liposome encapsulating fluorescent molecules with time course analysis
AU - Takada, K.
AU - Fujimoto, T.
AU - Shimanouchi, Toshinori
AU - Fukuzawa, M.
AU - Yamashita, K.
AU - Umakoshi, H.
AU - Noda, M.
PY - 2013
Y1 - 2013
N2 - This paper reports a new type of detection approach by arrayed biosensor for discrimination of target biomolecules such as protein and/or evaluation of their concentrations, utilizing liposome encapsulating fluorescent molecules and the corresponding time course analysis of the fluorescence. This method is realized by obtaining multi-dimensional data plots (curve) of fluorescent output such as its intensity from plural and different liposomes independent from each other. Two different proteins (carbonic anhydrase from bovine: CAB and Lysozyme), including their concentrations, were clearly discriminated by the developed method.
AB - This paper reports a new type of detection approach by arrayed biosensor for discrimination of target biomolecules such as protein and/or evaluation of their concentrations, utilizing liposome encapsulating fluorescent molecules and the corresponding time course analysis of the fluorescence. This method is realized by obtaining multi-dimensional data plots (curve) of fluorescent output such as its intensity from plural and different liposomes independent from each other. Two different proteins (carbonic anhydrase from bovine: CAB and Lysozyme), including their concentrations, were clearly discriminated by the developed method.
KW - Discrimination method
KW - Fluorescence
KW - Liposome
KW - Protein
KW - Sensor array
KW - Target biomolecule
UR - http://www.scopus.com/inward/record.url?scp=84907370112&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907370112&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84907370112
SN - 9781632666246
VL - 1
SP - 194
EP - 196
BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PB - Chemical and Biological Microsystems Society
ER -