A new discrimination method of target biomolecules with miniaturized sensor array utilizing liposome encapsulating fluorescent molecules with time course analysis

K. Takada, T. Fujimoto, T. Shimanouchi, M. Fukuzawa, K. Yamashita, H. Umakoshi, M. Noda

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

This paper reports a new type of detection approach by arrayed biosensor for discrimination of target biomolecules such as protein and/or evaluation of their concentrations, utilizing liposome encapsulating fluorescent molecules and the corresponding time course analysis of the fluorescence. This method is realized by obtaining multi-dimensional data plots (curve) of fluorescent output such as its intensity from plural and different liposomes independent from each other. Two different proteins (carbonic anhydrase from bovine: CAB and Lysozyme), including their concentrations, were clearly discriminated by the developed method.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages194-196
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - Jan 1 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

Keywords

  • Discrimination method
  • Fluorescence
  • Liposome
  • Protein
  • Sensor array
  • Target biomolecule

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    Takada, K., Fujimoto, T., Shimanouchi, T., Fukuzawa, M., Yamashita, K., Umakoshi, H., & Noda, M. (2013). A new discrimination method of target biomolecules with miniaturized sensor array utilizing liposome encapsulating fluorescent molecules with time course analysis. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 194-196). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1). Chemical and Biological Microsystems Society.