A new approach to inhibiting astrocytic IP3-induced intracellular calcium increase in an astrocyte-neuron co-culture system

Yasunori Saheki, Sheng Tian Li, Masayuki Matsushita, Yu Mei Wu, Wei Hua Cai, Fan Yan Wei, Yun Fei Lu, Akiyoshi Moriwaki, Kazuhito Tomizawa, Hideki Matsui

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Astrocytes exhibit dynamic Ca2+ mobilization, such as Ca 2+ wave and Ca2+ oscillation, via an inositol 1,4,5-triphosphate-induced Ca2+ release (IICR)-dependent mechanism. The physiological functions of astrocytic Ca2+ mobilization, however, are poorly understood. To investigate this issue, we created a plasmid encoding an enhanced green fluorescent protein-tagged inositol 1,4,5-triphosphate absorbent protein and expressed it in cultured astrocytes. Expression of this protein inhibited both IICR and the Ca2+ wave in cultured astrocytes. By combining this method to the single cell electroporation technique, we were able to inhibit IICR specifically in astrocytes in an astrocyte-neuron co-culture system. Our approach provides a useful tool for direct examination of the physiological role of astrocytic Ca2+ signaling on neuronal function.

Original languageEnglish
Pages (from-to)196-201
Number of pages6
JournalBrain Research
Issue number1-2
Publication statusPublished - Sep 7 2005


  • Astrocyte
  • Calcium wave
  • IP3
  • IP3 scavenger
  • Single cell electroporation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


Dive into the research topics of 'A new approach to inhibiting astrocytic IP<sub>3</sub>-induced intracellular calcium increase in an astrocyte-neuron co-culture system'. Together they form a unique fingerprint.

Cite this