A neonate with respiratory syncytial virus infection diagnosed by reverse transcriptase-polymerase chain reaction

Hiroyuki Yoshio, Makoto Nakamura, Yoshitada Yamauchi, Masao Yamada, Shirou Nii

Research output: Contribution to journalArticle

Abstract

We experienced a neonatal respiratory syncytial virus (RSV) infection diagnoed by reverse trancriptase-polymerase chain reaction (RT-PCR) method. The patient was a sixteen-day boy, and hospitalized to NICU with complaints of apneic spell and cough. Standard enzyme immunoassay (EIA) method (Abbott Test Pack RSV, Dainabot Co.) using nasopharyngeal secretion was performed, and the result was negative for the first three days of admission. On the other hand, RT-PCR using the primer pairs which Paton et al and the authors had pointed out was positive for the first five hospital days. The PCR products were confirmed by nested double RT-PCR. RSV isolation was also positive. We concluded that RT-PCR for RSV was useful and available of diagnosing RSV infection when the suspected case was negative by ETA.

Original languageEnglish
Pages (from-to)773-776
Number of pages4
JournalJapanese Journal of National Medical Services
Volume49
Issue number9
DOIs
Publication statusPublished - 1995

Fingerprint

Respiratory Syncytial Virus Infections
Reverse Transcriptase Polymerase Chain Reaction
Newborn Infant
Respiratory Syncytial Viruses
Polymerase Chain Reaction
Immunoenzyme Techniques
Cough

Keywords

  • EIA
  • neonate
  • RS virus
  • RT-PCR

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A neonate with respiratory syncytial virus infection diagnosed by reverse transcriptase-polymerase chain reaction. / Yoshio, Hiroyuki; Nakamura, Makoto; Yamauchi, Yoshitada; Yamada, Masao; Nii, Shirou.

In: Japanese Journal of National Medical Services, Vol. 49, No. 9, 1995, p. 773-776.

Research output: Contribution to journalArticle

Yoshio, Hiroyuki ; Nakamura, Makoto ; Yamauchi, Yoshitada ; Yamada, Masao ; Nii, Shirou. / A neonate with respiratory syncytial virus infection diagnosed by reverse transcriptase-polymerase chain reaction. In: Japanese Journal of National Medical Services. 1995 ; Vol. 49, No. 9. pp. 773-776.
@article{cf65959b9fa14f1da5f1af81410ff9b6,
title = "A neonate with respiratory syncytial virus infection diagnosed by reverse transcriptase-polymerase chain reaction",
abstract = "We experienced a neonatal respiratory syncytial virus (RSV) infection diagnoed by reverse trancriptase-polymerase chain reaction (RT-PCR) method. The patient was a sixteen-day boy, and hospitalized to NICU with complaints of apneic spell and cough. Standard enzyme immunoassay (EIA) method (Abbott Test Pack RSV, Dainabot Co.) using nasopharyngeal secretion was performed, and the result was negative for the first three days of admission. On the other hand, RT-PCR using the primer pairs which Paton et al and the authors had pointed out was positive for the first five hospital days. The PCR products were confirmed by nested double RT-PCR. RSV isolation was also positive. We concluded that RT-PCR for RSV was useful and available of diagnosing RSV infection when the suspected case was negative by ETA.",
keywords = "EIA, neonate, RS virus, RT-PCR",
author = "Hiroyuki Yoshio and Makoto Nakamura and Yoshitada Yamauchi and Masao Yamada and Shirou Nii",
year = "1995",
doi = "10.11261/iryo1946.49.773",
language = "English",
volume = "49",
pages = "773--776",
journal = "IRYO - Japanese Journal of National Medical Services",
issn = "0021-1699",
publisher = "Japanese Society of National Medical Services",
number = "9",

}

TY - JOUR

T1 - A neonate with respiratory syncytial virus infection diagnosed by reverse transcriptase-polymerase chain reaction

AU - Yoshio, Hiroyuki

AU - Nakamura, Makoto

AU - Yamauchi, Yoshitada

AU - Yamada, Masao

AU - Nii, Shirou

PY - 1995

Y1 - 1995

N2 - We experienced a neonatal respiratory syncytial virus (RSV) infection diagnoed by reverse trancriptase-polymerase chain reaction (RT-PCR) method. The patient was a sixteen-day boy, and hospitalized to NICU with complaints of apneic spell and cough. Standard enzyme immunoassay (EIA) method (Abbott Test Pack RSV, Dainabot Co.) using nasopharyngeal secretion was performed, and the result was negative for the first three days of admission. On the other hand, RT-PCR using the primer pairs which Paton et al and the authors had pointed out was positive for the first five hospital days. The PCR products were confirmed by nested double RT-PCR. RSV isolation was also positive. We concluded that RT-PCR for RSV was useful and available of diagnosing RSV infection when the suspected case was negative by ETA.

AB - We experienced a neonatal respiratory syncytial virus (RSV) infection diagnoed by reverse trancriptase-polymerase chain reaction (RT-PCR) method. The patient was a sixteen-day boy, and hospitalized to NICU with complaints of apneic spell and cough. Standard enzyme immunoassay (EIA) method (Abbott Test Pack RSV, Dainabot Co.) using nasopharyngeal secretion was performed, and the result was negative for the first three days of admission. On the other hand, RT-PCR using the primer pairs which Paton et al and the authors had pointed out was positive for the first five hospital days. The PCR products were confirmed by nested double RT-PCR. RSV isolation was also positive. We concluded that RT-PCR for RSV was useful and available of diagnosing RSV infection when the suspected case was negative by ETA.

KW - EIA

KW - neonate

KW - RS virus

KW - RT-PCR

UR - http://www.scopus.com/inward/record.url?scp=85007730583&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85007730583&partnerID=8YFLogxK

U2 - 10.11261/iryo1946.49.773

DO - 10.11261/iryo1946.49.773

M3 - Article

AN - SCOPUS:85007730583

VL - 49

SP - 773

EP - 776

JO - IRYO - Japanese Journal of National Medical Services

JF - IRYO - Japanese Journal of National Medical Services

SN - 0021-1699

IS - 9

ER -