Abstract
We identified a novel mutation of a nuclear-encoded gene, designated as CRUMPLED LEAF (CRL), of Arabidopsis thaliana that affects the morphogenesis of all plant organs and division of plastids. Histological analysis revealed that planes of cell division were distorted in shoot apical meristems (SAMs), root tips, and embryos in plants that possess the crl mutation. Furthermore, we observed that differentiation patterns of cortex and endodermis cells in inflorescence stems and root endodermis cells were disturbed in the crl mutant. These results suggest that morphological abnormalities observed in the crl mutant were because of aberrant cell division and differentiation. In addition, cells of the crl mutant contained a reduced number of enlarged plastids, indicating that the division of plastids was inhibited in the crl. The CRL gene encodes a novel protein with a molecular mass of 30 kDa that is localized in the plastid envelope. The CRL protein is conserved in various plant species, including a fern, and in cyanobacteria, but not in other organisms. These data suggest that the CRL protein is required for plastid division, and it also plays an important role in cell differentiation and the regulation of the cell division plane in plants. A possible function of the CRL protein is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 448-459 |
| Number of pages | 12 |
| Journal | Plant Journal |
| Volume | 38 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - May 2004 |
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Keywords
- Arabidopsis
- Cell differentiation
- Cell division plane
- Chloroplast division
- Envelope membrane
ASJC Scopus subject areas
- Plant Science
Cite this
A mutation of the Crumpled Leaf gene that encodes a protein localized in the outer envelope membrane of plastids affects the pattern of cell division, cell differentiation, and plastid division in Arabidopsis. / Asano, Tomoya; Yoshioka, Yasushi; Kurei, Shunsuke; Sakamoto, Wataru; Sodmergen; Machida, Yasunori.
In: Plant Journal, Vol. 38, No. 3, 05.2004, p. 448-459.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A mutation of the Crumpled Leaf gene that encodes a protein localized in the outer envelope membrane of plastids affects the pattern of cell division, cell differentiation, and plastid division in Arabidopsis
AU - Asano, Tomoya
AU - Yoshioka, Yasushi
AU - Kurei, Shunsuke
AU - Sakamoto, Wataru
AU - Sodmergen,
AU - Machida, Yasunori
PY - 2004/5
Y1 - 2004/5
N2 - We identified a novel mutation of a nuclear-encoded gene, designated as CRUMPLED LEAF (CRL), of Arabidopsis thaliana that affects the morphogenesis of all plant organs and division of plastids. Histological analysis revealed that planes of cell division were distorted in shoot apical meristems (SAMs), root tips, and embryos in plants that possess the crl mutation. Furthermore, we observed that differentiation patterns of cortex and endodermis cells in inflorescence stems and root endodermis cells were disturbed in the crl mutant. These results suggest that morphological abnormalities observed in the crl mutant were because of aberrant cell division and differentiation. In addition, cells of the crl mutant contained a reduced number of enlarged plastids, indicating that the division of plastids was inhibited in the crl. The CRL gene encodes a novel protein with a molecular mass of 30 kDa that is localized in the plastid envelope. The CRL protein is conserved in various plant species, including a fern, and in cyanobacteria, but not in other organisms. These data suggest that the CRL protein is required for plastid division, and it also plays an important role in cell differentiation and the regulation of the cell division plane in plants. A possible function of the CRL protein is discussed.
AB - We identified a novel mutation of a nuclear-encoded gene, designated as CRUMPLED LEAF (CRL), of Arabidopsis thaliana that affects the morphogenesis of all plant organs and division of plastids. Histological analysis revealed that planes of cell division were distorted in shoot apical meristems (SAMs), root tips, and embryos in plants that possess the crl mutation. Furthermore, we observed that differentiation patterns of cortex and endodermis cells in inflorescence stems and root endodermis cells were disturbed in the crl mutant. These results suggest that morphological abnormalities observed in the crl mutant were because of aberrant cell division and differentiation. In addition, cells of the crl mutant contained a reduced number of enlarged plastids, indicating that the division of plastids was inhibited in the crl. The CRL gene encodes a novel protein with a molecular mass of 30 kDa that is localized in the plastid envelope. The CRL protein is conserved in various plant species, including a fern, and in cyanobacteria, but not in other organisms. These data suggest that the CRL protein is required for plastid division, and it also plays an important role in cell differentiation and the regulation of the cell division plane in plants. A possible function of the CRL protein is discussed.
KW - Arabidopsis
KW - Cell differentiation
KW - Cell division plane
KW - Chloroplast division
KW - Envelope membrane
UR - http://www.scopus.com/inward/record.url?scp=2342525212&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2342525212&partnerID=8YFLogxK
U2 - 10.1111/j.1365-313X.2004.02057.x
DO - 10.1111/j.1365-313X.2004.02057.x
M3 - Article
C2 - 15086805
AN - SCOPUS:2342525212
VL - 38
SP - 448
EP - 459
JO - Plant Journal
JF - Plant Journal
SN - 0960-7412
IS - 3
ER -