A mutation in GIANT CHLOROPLAST encoding a PARC6 homolog affects spikelet fertility in rice

Peter K. Kamau, Shingo Sano, Tsuneaki Takami, Ryo Matsushima, Masahiko Maekawa, Wataru Sakamoto

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Chloroplasts are not generated de novo but proliferate from a pre-existing population of plastids present in meristematic cells. Chloroplast division is executed by the co-ordinated action of at least two molecular machineries: internal machinery located on the stromal side of the inner envelope membrane and external machinery located on the cytosolic side of the outer envelope membrane. To date, molecular studies of chloroplast division in higher plants have been limited to several species such as Arabidopsis. To elucidate chloroplast division in rice, we performed forward genetics and isolated a mutant displaying large chloroplasts among an ethyl methanesulfonate (EMS)-mutagenized Oryza sativa spp japonica Nipponbare population. Using a map-based approach, this mutation, termed giant chloroplast (gic), was allocated in a gene that encodes a protein that is homologous to Paralog of ARC6 (PARC6), which is known to play a role in chloroplast division. GIC is unique in that it has a long C-terminal extension that is not present in other PARC6 homologs. Characterization of gic phenotypes in a rice field showed that gic exhibited defective growth in seed setting, suggesting that the gic mutant negatively affects the reproductive stage. This report is the first describing a chloroplast division mutant in monocotyledons and its effect on plant development.

Original languageEnglish
Pages (from-to)977-991
Number of pages15
JournalPlant and Cell Physiology
Volume56
Issue number5
DOIs
Publication statusPublished - Nov 17 2014

Fingerprint

Chloroplasts
Fertility
inflorescences
chloroplasts
mutation
rice
Mutation
mutants
Oryza
Ethyl Methanesulfonate
ethyl methanesulfonate
Plastids
Plant Development
Membranes
Liliopsida
Arabidopsis
Population
paddies
plastids
plant development

Keywords

  • Chloroplast
  • Division ring
  • GIANT CHLOROPLAST
  • Monocots
  • Oryza sativa
  • PARC6

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

A mutation in GIANT CHLOROPLAST encoding a PARC6 homolog affects spikelet fertility in rice. / Kamau, Peter K.; Sano, Shingo; Takami, Tsuneaki; Matsushima, Ryo; Maekawa, Masahiko; Sakamoto, Wataru.

In: Plant and Cell Physiology, Vol. 56, No. 5, 17.11.2014, p. 977-991.

Research output: Contribution to journalArticle

Kamau, Peter K. ; Sano, Shingo ; Takami, Tsuneaki ; Matsushima, Ryo ; Maekawa, Masahiko ; Sakamoto, Wataru. / A mutation in GIANT CHLOROPLAST encoding a PARC6 homolog affects spikelet fertility in rice. In: Plant and Cell Physiology. 2014 ; Vol. 56, No. 5. pp. 977-991.
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