A mutant strain of Chlamydomonas reinhardtii lacking the chloroplast photosystem II psbI gene grows photoautotrophically

P. Kunstner, A. Guardiola, Yuichiro Takahashi, J. D. Rochaix

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The product of the chloroplast psbI gene is associated with the photosystem II reaction center. To gain insights into the function of this polypeptide, we have disrupted its gene in Chlamydomonas reinhardtii with an aadA expression cassette that confers resistance to spectinomycin through biolistic transformation. The transformants are still able to grow photoautotrophically in dim light, but not in high light, and they remain photosensitive when grown on acetate containing medium. The amounts of photosystem II complex and oxygen evolving activity are both reduced to 10- 20% of wild-type levels in these psbI-deficient mutants. It appears that the PsbI polypeptide plays a role in the stability of photosystem II and possibly also in modulating electron transport or energy transfer in this complex.

Original languageEnglish
Pages (from-to)9651-9654
Number of pages4
JournalJournal of Biological Chemistry
Volume270
Issue number16
DOIs
Publication statusPublished - 1995
Externally publishedYes

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Chlamydomonas reinhardtii
Photosystem II Protein Complex
Chloroplasts
Genes
Biolistics
Chloroplast Genes
Spectinomycin
Light
Peptides
Energy Transfer
Electron Transport
Energy transfer
Acetates
Oxygen

ASJC Scopus subject areas

  • Biochemistry

Cite this

A mutant strain of Chlamydomonas reinhardtii lacking the chloroplast photosystem II psbI gene grows photoautotrophically. / Kunstner, P.; Guardiola, A.; Takahashi, Yuichiro; Rochaix, J. D.

In: Journal of Biological Chemistry, Vol. 270, No. 16, 1995, p. 9651-9654.

Research output: Contribution to journalArticle

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