A multi-year assessment of the environmental impact of transgenic Eucalyptus trees harboring a bacterial choline oxidase gene on biomass, precinct vegetation and the microbial community

Taichi Oguchi, Yuko Kashimura, Makiko Mimura, Xiang Yu, Etsuko Matsunaga, Kazuya Nanto, Teruhisa Shimada, Akira Kikuchi, Kazuo N. Watanabe

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A 4-year field trial for the salt tolerant Eucalyptus globulus Labill. harboring the choline oxidase (codA) gene derived from the halobacterium Arthrobacter globiformis was conducted to assess the impact of transgenic versus non-transgenic trees on biomass production, the adjacent soil microbial communities and vegetation by monitoring growth parameters, seasonal changes in soil microbes and the allelopathic activity of leaves. Three independently-derived lines of transgenic E. globulus were compared with three independent non-transgenic lines including two elite clones. No significant differences in biomass production were detected between transgenic lines and non-transgenic controls derived from same seed bulk, while differences were seen compared to two elite clones. Significant differences in the number of soil microbes present were also detected at different sampling times but not between transgenic and non-transgenic lines. The allelopathic activity of leaves from both transgenic and non-transgenic lines also varied significantly with sampling time, but the allelopathic activity of leaves from transgenic lines did not differ significantly from those from non-transgenic lines. These results indicate that, for the observed variables, the impact on the environment of codA-transgenic E. globulus did not differ significantly from that of the non-transformed controls on this field trial.

Original languageEnglish
Pages (from-to)767-777
Number of pages11
JournalTransgenic Research
Volume23
Issue number5
DOIs
Publication statusPublished - Oct 1 2014
Externally publishedYes

Fingerprint

choline oxidase
Eucalyptus
environmental assessment
Biomass
microbial communities
Soil
genetically modified organisms
vegetation
biomass
Clone Cells
Halobacterium
Eucalyptus globulus
allelopathy
Genes
Arthrobacter
genes
soil microorganisms
Seeds
Salts
biomass production

Keywords

  • Biomass
  • Choline oxidase (codA)
  • Environmental risk assessment (ERA)
  • Eucalyptus
  • Field trial

ASJC Scopus subject areas

  • Biotechnology
  • Animal Science and Zoology
  • Genetics
  • Agronomy and Crop Science

Cite this

A multi-year assessment of the environmental impact of transgenic Eucalyptus trees harboring a bacterial choline oxidase gene on biomass, precinct vegetation and the microbial community. / Oguchi, Taichi; Kashimura, Yuko; Mimura, Makiko; Yu, Xiang; Matsunaga, Etsuko; Nanto, Kazuya; Shimada, Teruhisa; Kikuchi, Akira; Watanabe, Kazuo N.

In: Transgenic Research, Vol. 23, No. 5, 01.10.2014, p. 767-777.

Research output: Contribution to journalArticle

Oguchi, Taichi ; Kashimura, Yuko ; Mimura, Makiko ; Yu, Xiang ; Matsunaga, Etsuko ; Nanto, Kazuya ; Shimada, Teruhisa ; Kikuchi, Akira ; Watanabe, Kazuo N. / A multi-year assessment of the environmental impact of transgenic Eucalyptus trees harboring a bacterial choline oxidase gene on biomass, precinct vegetation and the microbial community. In: Transgenic Research. 2014 ; Vol. 23, No. 5. pp. 767-777.
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