A mouse model of orthotopic vascularized aerated lung transplantation

M. Okazaki, A. S. Krupnick, C. G. Kornfeld, J. M. Lai, J. H. Ritter, S. B. Richardson, H. J. Huang, N. A. Das, G. A. Patterson, A. E. Gelman, D. Kreisel

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Outcomes after lung transplantation are markedly inferior to those after other solid organ transplants. A better understanding of cellular and molecular mechanisms contributing to lung graft injury will be critical to improve outcomes. Advances in this field have been hampered by the lack of a mouse model of lung transplantation. Here, we report a mouse model of vascularized aerated single lung transplantation utilizing cuff techniques. We show that syngeneic grafts have normal histological appearance with minimal infiltration of T lymphocytes. Allogeneic grafts show acute cellular rejection with infiltration of T lymphocytes and recipient-type antigen presenting cells. Our data show that we have developed a physiological model of lung transplantation in the mouse, which provides ample opportunity for the study of nonimmune and immune mechanisms that contribute to lung allograft injury.

Original languageEnglish
Pages (from-to)1672-1679
Number of pages8
JournalAmerican Journal of Transplantation
Volume7
Issue number6
DOIs
Publication statusPublished - Jun 1 2007
Externally publishedYes

Keywords

  • Lung transplantation
  • Mice
  • Surgical technique

ASJC Scopus subject areas

  • Immunology and Allergy
  • Transplantation
  • Pharmacology (medical)

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  • Cite this

    Okazaki, M., Krupnick, A. S., Kornfeld, C. G., Lai, J. M., Ritter, J. H., Richardson, S. B., Huang, H. J., Das, N. A., Patterson, G. A., Gelman, A. E., & Kreisel, D. (2007). A mouse model of orthotopic vascularized aerated lung transplantation. American Journal of Transplantation, 7(6), 1672-1679. https://doi.org/10.1111/j.1600-6143.2007.01819.x