A morphological study on glutamate-induced swelling of cultured astrocytes: Involvement of calcium and chloride ion mechanisms

Yutaka Koyama, Tomosada Sugimoto, Yoshio Shigenaga, Akemichi Baba, Heitaroh Iwata

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Morphological changes in cultured astrocytes exposed to l-glutamate (Glu) were examined light and electron microscopically. The treatment with 0.1 mM Glu for 60 min caused marked swelling of the cells, which was characterized by reduction in staining of cytoplasm with Toluidine blue, disappearance of the cytoplasmic granular ground substances, swollen mitochondrion and nucleus, and dispersed chromatin. The above changes were prevented by the removal of Na+, Ca2+ or Cl- from the incubation medium for Glu treatment. However, the Glu treatment in a Cl--free medium caused conspicuous aggregation of 10 nm filaments.

Original languageEnglish
Pages (from-to)235-238
Number of pages4
JournalNeuroscience Letters
Volume124
Issue number2
DOIs
Publication statusPublished - Apr 1 1991

Keywords

  • Astrocyte
  • Ca
  • Swelling
  • Ultrastructure
  • l-Glutamate

ASJC Scopus subject areas

  • Neuroscience(all)

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