Abstract
A monoclonal antibody (MAb-1D10), generated by immunizing Balb/c mice with homogenized bovine retinal tissue, labeled the Müller cells in the bovine retina and the Purkinje cells of the rat cerebellum. In the neonatal rat cerebellum, immunoreactivity for MAb-1D10 appeared first in the ventral regions of vermis (lobuli I-III and IX-X) on the 11th postnatal day (P11), while in the dorsal vermis and cerebellar hemisphere, the immunoreactive Purkinje cells were detected on P13. Western blotting analysis disclosed that MAb-1D10 recognized bovine retinal polypeptides with mol.wt. of 48 kDa. Transplantation of the rat cerebellar primordia on 16th embryonic day demonstrated large and round cells with a linear arrangement in the grafts surviving well in the host rat striatum after 50 days of the grafting. These large cells were immunohistochemically labeled by MAb-1D10. No other cells in both the graft and the host striatum were immunoreactive to the MAb.
Original language | English |
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Pages (from-to) | 669-674 |
Number of pages | 6 |
Journal | Brain Research Bulletin |
Volume | 27 |
Issue number | 5 |
DOIs | |
Publication status | Published - Nov 1991 |
Keywords
- Bovine retina
- Cerebellar grafting
- Monoclonal antibody
- Purkinje cells
- Rat cerebellum
ASJC Scopus subject areas
- Neuroscience(all)