A Microbial rhodopsin with a unique retinal composition shows both sensory rhodopsin II and bacteriorhodopsin-like properties

Yuki Sudo, Kunio Ihara, Shiori Kobayashi, Daisuke Suzuki, Hiroki Irieda, Takashi Kikukawa, Hideki Kandori, Michio Homma

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Rhodopsins possess retinal chromophore surrounded by seven transmembrane α-helices, are widespread in prokaryotes and in eukaryotes, and can be utilized as optogenetic tools. Although rhodopsins work as distinctly different photo-receptors in various organisms, they can be roughly divided according to their two basic functions, light-energy conversion and light-signal transduction. In microbes, light-driven proton transporters functioning as light-energy converters have been modified by evolution to produce sensory receptors that relay signals to transducer proteins to control motility. In this study, we cloned and characterized two newly identified microbial rhodopsins from Haloquadratum walsbyi. One of them has photochemical properties and a proton pumping activity similar to the well known proton pump bacteriorhodopsin (BR). The other, named middle rhodopsin (MR), is evolutionarily transitional between BR and the phototactic sensory rhodopsin II (SRII), having an SRII-like absorption maximum, a BR-like photocycle, and a unique retinal composition. The wild-type MR does not have a light-induced proton pumping activity. On the other hand, a mutant MR with two key hydrogen-bonding residues located at the interaction surface with the transducer protein HtrII shows robust phototaxis responses similar to SRII, indicating that MRis potentially capable of the signaling. These results demonstrate that color tuning and insertion of the critical threonine residue occurred early in the evolution of sensory rhodopsins. MRmay be a missing link in the evolution from type 1 rhodopsins (microorganisms) to type 2 rhodopsins (animals), because it is the first microbial rhodopsin known to have 11-cis-retinal similar to type 2 rhodopsins.

Original languageEnglish
Pages (from-to)5967-5976
Number of pages10
JournalJournal of Biological Chemistry
Volume286
Issue number8
DOIs
Publication statusPublished - Feb 25 2011
Externally publishedYes

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Microbial Rhodopsins
Sensory Rhodopsins
Bacteriorhodopsins
Rhodopsin
Chemical analysis
Protons
Light
Transducers
Optogenetics
Retinaldehyde
Light Signal Transduction
Proton Pumps
Signal transduction
Sensory Receptor Cells
Threonine
Hydrogen Bonding
Chromophores
Eukaryota
Energy conversion
Microorganisms

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

A Microbial rhodopsin with a unique retinal composition shows both sensory rhodopsin II and bacteriorhodopsin-like properties. / Sudo, Yuki; Ihara, Kunio; Kobayashi, Shiori; Suzuki, Daisuke; Irieda, Hiroki; Kikukawa, Takashi; Kandori, Hideki; Homma, Michio.

In: Journal of Biological Chemistry, Vol. 286, No. 8, 25.02.2011, p. 5967-5976.

Research output: Contribution to journalArticle

Sudo, Yuki ; Ihara, Kunio ; Kobayashi, Shiori ; Suzuki, Daisuke ; Irieda, Hiroki ; Kikukawa, Takashi ; Kandori, Hideki ; Homma, Michio. / A Microbial rhodopsin with a unique retinal composition shows both sensory rhodopsin II and bacteriorhodopsin-like properties. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 8. pp. 5967-5976.
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