A method for purification of Clostridium perfringens phospholipase C from recombinant Bacillus subtilis cells

Y. Hirata; J. Minami; M. Koyama; O. Matsushita; S. I. Katayama; F. Jin; H. Maeta; A. Okabe

doi:10.1128/aem.61.11.4114-4115.1995

A method for purification of Clostridium perfringens phospholipase C from recombinant Bacillus subtilis cells

Y. Hirata, J. Minami, M. Koyama, O. Matsushita, S. I. Katayama, F. Jin, H. Maeta, A. Okabe

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture.

Original language	English
Pages (from-to)	4114-4115
Number of pages	2
Journal	Applied and environmental microbiology
Volume	61
Issue number	11
DOIs	https://doi.org/10.1128/aem.61.11.4114-4115.1995
Publication status	Published - 1995
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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10.1128/aem.61.11.4114-4115.1995

Cite this

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title = "A method for purification of Clostridium perfringens phospholipase C from recombinant Bacillus subtilis cells",

abstract = "We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture.",

author = "Y. Hirata and J. Minami and M. Koyama and O. Matsushita and Katayama, {S. I.} and F. Jin and H. Maeta and A. Okabe",

year = "1995",

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AU - Hirata, Y.

AU - Minami, J.

AU - Koyama, M.

AU - Matsushita, O.

AU - Katayama, S. I.

AU - Jin, F.

AU - Maeta, H.

AU - Okabe, A.

PY - 1995

Y1 - 1995

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AB - We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture.

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M3 - Article

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AN - SCOPUS:0028862923

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JO - Applied and Environmental Microbiology

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ER -

A method for purification of Clostridium perfringens phospholipase C from recombinant Bacillus subtilis cells

Abstract

ASJC Scopus subject areas

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