A method for purification of Clostridium perfringens phospholipase C from recombinant Bacillus subtilis cells

Y. Hirata, J. Minami, M. Koyama, Osamu Matsushita, S. I. Katayama, F. Jin, H. Maeta, A. Okabe

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture.

Original languageEnglish
Pages (from-to)4114-4115
Number of pages2
JournalApplied and Environmental Microbiology
Volume61
Issue number11
Publication statusPublished - 1995
Externally publishedYes

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purification methods
Clostridium perfringens
phospholipase C
Type C Phospholipases
Bacillus subtilis
purification
cells
methodology
method

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science(all)
  • Microbiology

Cite this

A method for purification of Clostridium perfringens phospholipase C from recombinant Bacillus subtilis cells. / Hirata, Y.; Minami, J.; Koyama, M.; Matsushita, Osamu; Katayama, S. I.; Jin, F.; Maeta, H.; Okabe, A.

In: Applied and Environmental Microbiology, Vol. 61, No. 11, 1995, p. 4114-4115.

Research output: Contribution to journalArticle

Hirata, Y, Minami, J, Koyama, M, Matsushita, O, Katayama, SI, Jin, F, Maeta, H & Okabe, A 1995, 'A method for purification of Clostridium perfringens phospholipase C from recombinant Bacillus subtilis cells', Applied and Environmental Microbiology, vol. 61, no. 11, pp. 4114-4115.
Hirata, Y. ; Minami, J. ; Koyama, M. ; Matsushita, Osamu ; Katayama, S. I. ; Jin, F. ; Maeta, H. ; Okabe, A. / A method for purification of Clostridium perfringens phospholipase C from recombinant Bacillus subtilis cells. In: Applied and Environmental Microbiology. 1995 ; Vol. 61, No. 11. pp. 4114-4115.
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