We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture.
|Number of pages||2|
|Journal||Applied and environmental microbiology|
|Publication status||Published - Jan 1 1995|
ASJC Scopus subject areas
- Food Science
- Applied Microbiology and Biotechnology