Abstract
We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture.
| Original language | English |
|---|---|
| Pages (from-to) | 4114-4115 |
| Number of pages | 2 |
| Journal | Applied and environmental microbiology |
| Volume | 61 |
| Issue number | 11 |
| Publication status | Published - Jan 1 1995 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biotechnology
- Food Science
- Applied Microbiology and Biotechnology
- Ecology
Cite this
Hirata, Y., Minami, J., Koyama, M., Matsushita, O., Katayama, S. I., Jin, F., Maeta, H., & Okabe, A. (1995). A method for purification of Clostridium perfringens phospholipase C from recombinant Bacillus subtilis cells. Applied and environmental microbiology, 61(11), 4114-4115.