Abstract
We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture.
| Original language | English |
|---|---|
| Pages (from-to) | 4114-4115 |
| Number of pages | 2 |
| Journal | Applied and Environmental Microbiology |
| Volume | 61 |
| Issue number | 11 |
| Publication status | Published - 1995 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biotechnology
- Environmental Science(all)
- Microbiology
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A method for purification of Clostridium perfringens phospholipase C from recombinant Bacillus subtilis cells. / Hirata, Y.; Minami, J.; Koyama, M.; Matsushita, Osamu; Katayama, S. I.; Jin, F.; Maeta, H.; Okabe, A.
In: Applied and Environmental Microbiology, Vol. 61, No. 11, 1995, p. 4114-4115.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A method for purification of Clostridium perfringens phospholipase C from recombinant Bacillus subtilis cells
AU - Hirata, Y.
AU - Minami, J.
AU - Koyama, M.
AU - Matsushita, Osamu
AU - Katayama, S. I.
AU - Jin, F.
AU - Maeta, H.
AU - Okabe, A.
PY - 1995
Y1 - 1995
N2 - We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture.
AB - We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture.
UR - http://www.scopus.com/inward/record.url?scp=0028862923&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028862923&partnerID=8YFLogxK
M3 - Article
VL - 61
SP - 4114
EP - 4115
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 11
ER -