A method for purification of Clostridium perfringens phospholipase C from recombinant Bacillus subtilis cells

Y. Hirata, J. Minami, M. Koyama, O. Matsushita, S. I. Katayama, F. Jin, H. Maeta, A. Okabe

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Abstract

We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture.

Original languageEnglish
Pages (from-to)4114-4115
Number of pages2
JournalApplied and environmental microbiology
Volume61
Issue number11
Publication statusPublished - Jan 1 1995
Externally publishedYes

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ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Hirata, Y., Minami, J., Koyama, M., Matsushita, O., Katayama, S. I., Jin, F., Maeta, H., & Okabe, A. (1995). A method for purification of Clostridium perfringens phospholipase C from recombinant Bacillus subtilis cells. Applied and environmental microbiology, 61(11), 4114-4115.