A metallothionein and CPx-ATPase handle heavy-metal tolerance in the filamentous cyanobacterium Oscillatoria brevis

Tong Liu; Susumu Nakashima; Kazunobu Hirose; Yoshiko Uemura; Mineo Shibasaka; Maki Katsuhara; Kunihiro Kasamo

doi:10.1016/S0014-5793(03)00370-3

A metallothionein and CPx-ATPase handle heavy-metal tolerance in the filamentous cyanobacterium Oscillatoria brevis

Tong Liu, Susumu Nakashima, Kazunobu Hirose, Yoshiko Uemura, Mineo Shibasaka, Maki Katsuhara, Kunihiro Kasamo

Institute of Plant Science and Resources

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

A metallothionein (BmtA) and a CPx-ATPase (Bxa1) have been identified and characterized from the cyanobacterium Oscillatoria brevis. Both bmtA and bxa1 expression can be markedly induced in vivo by Zn²⁺ or Cd²⁺. Over-expression of bmtA or bxa1 in Escherichia coli enhances Zn²⁺ and Cd²⁺ tolerance in the transformant. Dynamic studies on the expression of two genes showed that the maximum expression of bxa1 induced by Zn²⁺ and Cd²⁺ was much quicker than that of bmtA, suggesting distinct physiological roles of metallothionein and CPx-ATPase in the handling of surplus metal.

Original language	English
Pages (from-to)	159-163
Number of pages	5
Journal	FEBS Letters
Volume	542
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(03)00370-3
Publication status	Published - May 8 2003

Keywords

CPx-ATPase
Cyanobacterium
Heavy metal tolerance
Metallothionein

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "A metallothionein and CPx-ATPase handle heavy-metal tolerance in the filamentous cyanobacterium Oscillatoria brevis",

abstract = "A metallothionein (BmtA) and a CPx-ATPase (Bxa1) have been identified and characterized from the cyanobacterium Oscillatoria brevis. Both bmtA and bxa1 expression can be markedly induced in vivo by Zn2+ or Cd2+. Over-expression of bmtA or bxa1 in Escherichia coli enhances Zn2+ and Cd2+ tolerance in the transformant. Dynamic studies on the expression of two genes showed that the maximum expression of bxa1 induced by Zn2+ and Cd2+ was much quicker than that of bmtA, suggesting distinct physiological roles of metallothionein and CPx-ATPase in the handling of surplus metal.",

keywords = "CPx-ATPase, Cyanobacterium, Heavy metal tolerance, Metallothionein",

author = "Tong Liu and Susumu Nakashima and Kazunobu Hirose and Yoshiko Uemura and Mineo Shibasaka and Maki Katsuhara and Kunihiro Kasamo",

note = "Funding Information: This work was partially supported by grants from the Sumitomo Foundation (to S.N.), from the Ryobi Foundation (to S.N.), and from the Oohara Foundation for Agricultural Sciences and Grants-in-Aid for the Future Program from the Japanese Society for the Promotion of Science (no. JSPS-PFTF 9616001 to S.N.) and for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan. (no. 11440237 to K.K.; no. 10878087 to S.N.). We thank Dr. Hideo Okamura, as well as the Norwegian Institute for Water Research for providing O. brevis .",

year = "2003",

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T1 - A metallothionein and CPx-ATPase handle heavy-metal tolerance in the filamentous cyanobacterium Oscillatoria brevis

AU - Liu, Tong

AU - Nakashima, Susumu

AU - Hirose, Kazunobu

AU - Uemura, Yoshiko

AU - Shibasaka, Mineo

AU - Katsuhara, Maki

AU - Kasamo, Kunihiro

N1 - Funding Information: This work was partially supported by grants from the Sumitomo Foundation (to S.N.), from the Ryobi Foundation (to S.N.), and from the Oohara Foundation for Agricultural Sciences and Grants-in-Aid for the Future Program from the Japanese Society for the Promotion of Science (no. JSPS-PFTF 9616001 to S.N.) and for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan. (no. 11440237 to K.K.; no. 10878087 to S.N.). We thank Dr. Hideo Okamura, as well as the Norwegian Institute for Water Research for providing O. brevis .

PY - 2003/5/8

Y1 - 2003/5/8

N2 - A metallothionein (BmtA) and a CPx-ATPase (Bxa1) have been identified and characterized from the cyanobacterium Oscillatoria brevis. Both bmtA and bxa1 expression can be markedly induced in vivo by Zn2+ or Cd2+. Over-expression of bmtA or bxa1 in Escherichia coli enhances Zn2+ and Cd2+ tolerance in the transformant. Dynamic studies on the expression of two genes showed that the maximum expression of bxa1 induced by Zn2+ and Cd2+ was much quicker than that of bmtA, suggesting distinct physiological roles of metallothionein and CPx-ATPase in the handling of surplus metal.

AB - A metallothionein (BmtA) and a CPx-ATPase (Bxa1) have been identified and characterized from the cyanobacterium Oscillatoria brevis. Both bmtA and bxa1 expression can be markedly induced in vivo by Zn2+ or Cd2+. Over-expression of bmtA or bxa1 in Escherichia coli enhances Zn2+ and Cd2+ tolerance in the transformant. Dynamic studies on the expression of two genes showed that the maximum expression of bxa1 induced by Zn2+ and Cd2+ was much quicker than that of bmtA, suggesting distinct physiological roles of metallothionein and CPx-ATPase in the handling of surplus metal.

KW - CPx-ATPase

KW - Cyanobacterium

KW - Heavy metal tolerance

KW - Metallothionein

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