A metallothionein and CPx-ATPase handle heavy-metal tolerance in the filamentous cyanobacterium Oscillatoria brevis

Tong Liu, Susumu Nakashima, Kazunobu Hirose, Yoshiko Uemura, Mineo Shibasaka, Maki Katsuhara, Kunihiro Kasamo

Research output: Contribution to journal › Article

25 Citations (Scopus)

Abstract

A metallothionein (BmtA) and a CPx-ATPase (Bxa1) have been identified and characterized from the cyanobacterium Oscillatoria brevis. Both bmtA and bxa1 expression can be markedly induced in vivo by Zn2+ or Cd2+. Over-expression of bmtA or bxa1 in Escherichia coli enhances Zn2+ and Cd2+ tolerance in the transformant. Dynamic studies on the expression of two genes showed that the maximum expression of bxa1 induced by Zn2+ and Cd2+ was much quicker than that of bmtA, suggesting distinct physiological roles of metallothionein and CPx-ATPase in the handling of surplus metal.

Original languageEnglish
Pages (from-to)159-163
Number of pages5
JournalFEBS Letters
Volume542
Issue number1-3
DOIs
Publication statusPublished - May 8 2003

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Oscillatoria
Metallothionein
Cyanobacteria
Heavy Metals
Adenosine Triphosphatases
Escherichia coli
Genes
Metals
Gene Expression

Keywords

  • CPx-ATPase
  • Cyanobacterium
  • Heavy metal tolerance
  • Metallothionein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A metallothionein and CPx-ATPase handle heavy-metal tolerance in the filamentous cyanobacterium Oscillatoria brevis. / Liu, Tong; Nakashima, Susumu; Hirose, Kazunobu; Uemura, Yoshiko; Shibasaka, Mineo; Katsuhara, Maki; Kasamo, Kunihiro.

In: FEBS Letters, Vol. 542, No. 1-3, 08.05.2003, p. 159-163.

Research output: Contribution to journal › Article

Liu, T, Nakashima, S, Hirose, K, Uemura, Y, Shibasaka, M, Katsuhara, M & Kasamo, K 2003, 'A metallothionein and CPx-ATPase handle heavy-metal tolerance in the filamentous cyanobacterium Oscillatoria brevis', FEBS Letters, vol. 542, no. 1-3, pp. 159-163. https://doi.org/10.1016/S0014-5793(03)00370-3
Liu T, Nakashima S, Hirose K, Uemura Y, Shibasaka M, Katsuhara M et al. A metallothionein and CPx-ATPase handle heavy-metal tolerance in the filamentous cyanobacterium Oscillatoria brevis. FEBS Letters. 2003 May 8;542(1-3):159-163. https://doi.org/10.1016/S0014-5793(03)00370-3
Liu, Tong ; Nakashima, Susumu ; Hirose, Kazunobu ; Uemura, Yoshiko ; Shibasaka, Mineo ; Katsuhara, Maki ; Kasamo, Kunihiro. / A metallothionein and CPx-ATPase handle heavy-metal tolerance in the filamentous cyanobacterium Oscillatoria brevis. In: FEBS Letters. 2003 ; Vol. 542, No. 1-3. pp. 159-163.
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