Abstract
A metallothionein (BmtA) and a CPx-ATPase (Bxa1) have been identified and characterized from the cyanobacterium Oscillatoria brevis. Both bmtA and bxa1 expression can be markedly induced in vivo by Zn2+ or Cd2+. Over-expression of bmtA or bxa1 in Escherichia coli enhances Zn2+ and Cd2+ tolerance in the transformant. Dynamic studies on the expression of two genes showed that the maximum expression of bxa1 induced by Zn2+ and Cd2+ was much quicker than that of bmtA, suggesting distinct physiological roles of metallothionein and CPx-ATPase in the handling of surplus metal.
| Original language | English |
|---|---|
| Pages (from-to) | 159-163 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 542 |
| Issue number | 1-3 |
| DOIs | |
| Publication status | Published - May 8 2003 |
Keywords
- CPx-ATPase
- Cyanobacterium
- Heavy metal tolerance
- Metallothionein
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
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Cite this
Liu, T., Nakashima, S., Hirose, K., Uemura, Y., Shibasaka, M., Katsuhara, M., & Kasamo, K. (2003). A metallothionein and CPx-ATPase handle heavy-metal tolerance in the filamentous cyanobacterium Oscillatoria brevis. FEBS Letters, 542(1-3), 159-163. https://doi.org/10.1016/S0014-5793(03)00370-3