A metallothionein and CPx-ATPase handle heavy-metal tolerance in the filamentous cyanobacterium Oscillatoria brevis

Tong Liu, Susumu Nakashima, Kazunobu Hirose, Yoshiko Uemura, Mineo Shibasaka, Maki Katsuhara, Kunihiro Kasamo

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A metallothionein (BmtA) and a CPx-ATPase (Bxa1) have been identified and characterized from the cyanobacterium Oscillatoria brevis. Both bmtA and bxa1 expression can be markedly induced in vivo by Zn2+ or Cd2+. Over-expression of bmtA or bxa1 in Escherichia coli enhances Zn2+ and Cd2+ tolerance in the transformant. Dynamic studies on the expression of two genes showed that the maximum expression of bxa1 induced by Zn2+ and Cd2+ was much quicker than that of bmtA, suggesting distinct physiological roles of metallothionein and CPx-ATPase in the handling of surplus metal.

Original languageEnglish
Pages (from-to)159-163
Number of pages5
JournalFEBS Letters
Volume542
Issue number1-3
DOIs
Publication statusPublished - May 8 2003

Keywords

  • CPx-ATPase
  • Cyanobacterium
  • Heavy metal tolerance
  • Metallothionein

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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