A metallothionein and CPx-ATPase handle heavy-metal tolerance in the filamentous cyanobacterium Oscillatoria brevis

Tong Liu, Susumu Nakashima, Kazunobu Hirose, Yoshiko Uemura, Mineo Shibasaka, Maki Katsuhara, Kunihiro Kasamo

    Research output: Contribution to journalArticlepeer-review

    34 Citations (Scopus)

    Abstract

    A metallothionein (BmtA) and a CPx-ATPase (Bxa1) have been identified and characterized from the cyanobacterium Oscillatoria brevis. Both bmtA and bxa1 expression can be markedly induced in vivo by Zn2+ or Cd2+. Over-expression of bmtA or bxa1 in Escherichia coli enhances Zn2+ and Cd2+ tolerance in the transformant. Dynamic studies on the expression of two genes showed that the maximum expression of bxa1 induced by Zn2+ and Cd2+ was much quicker than that of bmtA, suggesting distinct physiological roles of metallothionein and CPx-ATPase in the handling of surplus metal.

    Original languageEnglish
    Pages (from-to)159-163
    Number of pages5
    JournalFEBS Letters
    Volume542
    Issue number1-3
    DOIs
    Publication statusPublished - May 8 2003

    Keywords

    • CPx-ATPase
    • Cyanobacterium
    • Heavy metal tolerance
    • Metallothionein

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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