A member of cation diffusion facilitator family, MTP11, is required for manganese tolerance and high fertility in rice

Yuta Tsunemitsu, Mayuko Genga, Tomoyuki Okada, Naoki Yamaji, Jian Feng Ma, Akira Miyazaki, Shin ichiro Kato, Kozo Iwasaki, Daisei Ueno

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Main conclusion: Rice MTP11 is the trans-Golgi-localized transporter that is involved in Mn tolerance with MTP8.1, and it is required for normal fertility. Rice (Oryza sativa L.) is one of the most manganese (Mn)-tolerant species, and it is able to accumulate high levels of this metal in the leaves without showing toxic symptoms. The metal tolerance protein 8.1 (MTP8.1), a member of the Mn-cation diffusion facilitator (CDF) family, has been shown to play a central role in high Mn tolerance by sequestering Mn into vacuoles. Recently, rice MTP11 was identified as an Mn transporter that is localized to Golgi-associated compartments, but its exact role in Mn tolerance in planta has not yet been understood. Here, we investigated the role of MTP11 in rice Mn tolerance using knockout lines. Old leaves presented higher levels of constitutively expressed MTP11 than other tissues and MTP11 expression was also found in reproductive organs. Fused MTP11:green fluorescent protein was co-localized to trans-Golgi markers and differentiated from other Golgi-associated markers. Knockout of MTP11 in wild-type rice did not affect tolerance and accumulation of Mn and other heavy metals, but knockout in the mtp8.1 mutant showed exacerbated Mn sensitivity at the vegetative growth stage. Knockout of MTP11 alone resulted in decreased grain yield and fertility at the reproductive stage. Thus, MTP11 is a trans-Golgi localized transporter for Mn, which plays a role in Mn tolerance through intracellular Mn compartmentalization. It is also required for maintaining high fertility in rice.

Original languageEnglish
Pages (from-to)231-241
Number of pages11
JournalPlanta
Volume248
Issue number1
DOIs
Publication statusPublished - Jul 1 2018

Fingerprint

Manganese
Fertility
manganese
Cations
cations
rice
transporters
metal tolerance
Metals
Oryza
knockout mutants
Poisons
Heavy Metals
Vacuoles
Plantae
Green Fluorescent Proteins
green fluorescent protein
signs and symptoms (plants)
vegetative growth
vacuoles

Keywords

  • Metal tolerance protein
  • Oryza sativa
  • Toxicity
  • trans-Golgi
  • Transporter

ASJC Scopus subject areas

  • Genetics
  • Plant Science

Cite this

A member of cation diffusion facilitator family, MTP11, is required for manganese tolerance and high fertility in rice. / Tsunemitsu, Yuta; Genga, Mayuko; Okada, Tomoyuki; Yamaji, Naoki; Ma, Jian Feng; Miyazaki, Akira; Kato, Shin ichiro; Iwasaki, Kozo; Ueno, Daisei.

In: Planta, Vol. 248, No. 1, 01.07.2018, p. 231-241.

Research output: Contribution to journalArticle

Tsunemitsu, Yuta ; Genga, Mayuko ; Okada, Tomoyuki ; Yamaji, Naoki ; Ma, Jian Feng ; Miyazaki, Akira ; Kato, Shin ichiro ; Iwasaki, Kozo ; Ueno, Daisei. / A member of cation diffusion facilitator family, MTP11, is required for manganese tolerance and high fertility in rice. In: Planta. 2018 ; Vol. 248, No. 1. pp. 231-241.
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AU - Yamaji, Naoki

AU - Ma, Jian Feng

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AU - Kato, Shin ichiro

AU - Iwasaki, Kozo

AU - Ueno, Daisei

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