A maize DNA-binding factor with a bZIP motif is induced by low temperature

Tomonobu Kusano, Thomas Berberich, Mariko Harada, Nobuhiro Suzuki, Kazuyuki Sugawara

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

We have isolated a low temperature-induced maize gene, m lip15, via cross hydridization using rice lip19. The longest cDNA isolated comprised 1179 bp and coded for a 135 amino acid bZIP (basic region/leucine zipper) protein. The gene showed 61.4% and 68.9% identity with the rice gene at the DNA and amino acid sequence levels, respectively, and is distinct from other maize genes that code for bZIP proteins. The level of m lip15 transcript was positively regulated by low temperature in the same way as the lip19 transcript. The levels of the transcript were also strongly increased by salt stress and exogenous abscisic acid, and slightly increased by anaerobiosis, but were not affected by heat shock and drought. The mLIP15 protein and truncated derivatives, produced in rabbit reticulocyte lysates or in an Escherichia coli expression system, were able to bind to a fragment of the wheat histone H3 gene promoter. This binding was diminished by addition of a molar excess of the hexamer sequence 5′-ACGTCA-3′ found in the promoter and of the G-box-like sequence, but not by the addition of the ocs sequence or a mutated hexamer sequence. The factor bound to a promoter region of the maize Adhl gene, expression of which is also induced by low temperature. These results lead to the conclusion that m lip15 is a strong candidate for a low temperature-induced transcription factor in maize.

Original languageEnglish
Pages (from-to)507-517
Number of pages11
JournalMGG Molecular & General Genetics
Volume248
Issue number5
DOIs
Publication statusPublished - Sep 1995
Externally publishedYes

Fingerprint

Zea mays
Temperature
DNA
Genes
Basic-Leucine Zipper Transcription Factors
Anaerobiosis
Leucine Zippers
Basic Amino Acids
Abscisic Acid
Droughts
Reticulocytes
Genetic Promoter Regions
Histones
Triticum
Amino Acid Sequence
Shock
Proteins
Transcription Factors
Complementary DNA
Salts

Keywords

  • bZIP protein
  • DNA-binding factor
  • Low temperature
  • Maize
  • Stress-induced gene

ASJC Scopus subject areas

  • Genetics

Cite this

A maize DNA-binding factor with a bZIP motif is induced by low temperature. / Kusano, Tomonobu; Berberich, Thomas; Harada, Mariko; Suzuki, Nobuhiro; Sugawara, Kazuyuki.

In: MGG Molecular & General Genetics, Vol. 248, No. 5, 09.1995, p. 507-517.

Research output: Contribution to journalArticle

Kusano, Tomonobu ; Berberich, Thomas ; Harada, Mariko ; Suzuki, Nobuhiro ; Sugawara, Kazuyuki. / A maize DNA-binding factor with a bZIP motif is induced by low temperature. In: MGG Molecular & General Genetics. 1995 ; Vol. 248, No. 5. pp. 507-517.
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