A magnesium transporter OsMGT1 plays a critical role in salt tolerance in rice

Zhi Chang Chen, Naoki Yamaji, Tomoaki Horie, Jing Che, Jian Li, Gynheung An, Jian Feng Ma

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Salt stress is one of the major factors limiting rice (Oryza sativa) production globally. Although several transporters involved in salt tolerance have been identified in rice, the mechanisms regulating their transport activity are still poorly understood. Here, we show evidence that a rice Mg transporter OsMGT1 is required for salt tolerance probably by regulating transport activity of OsHKT1;5, a key transporter for the removal of Na+ from the xylem sap at the root mature zone. Knockout of OsMGT1 did not affect total Na uptake, but increased Na concentration in the shoots and xylem sap, resulting in a significant increase in salt sensitivity at low external Mg2+ concentration (20–200 mM). However, such differences were abolished at a higher Mg2+ concentration (2 mM), although the total Na uptake was not altered. OsMGT1 was expressed in both the roots and shoots, but only that in the roots was moderately up-regulated by salt stress. Spatial expression analysis revealed that OsMGT1 was expressed in all root cells of the root tips but was highly expressed in the pericycle of root mature zone. OsMGT1 was also expressed in the phloem region of basal node, leaf blade, and sheath. When expressed in Xenopus laevis oocytes, the transport activity of OsHKT1;5 was enhanced by elevating external Mg2+ concentration. Furthermore, knockout of OsHKT1;5 in osmgt1 mutant background did not further increase its salt sensitivity. Taken together, our results suggest that Mg2+ transported by OsMGT1 in the root mature zone is required for enhancing OsHKT1;5 activity, thereby restricting Na accumulation to the shoots.

Original languageEnglish
Pages (from-to)1837-1849
Number of pages13
JournalPlant Physiology
Volume174
Issue number3
DOIs
Publication statusPublished - Jul 1 2017

Fingerprint

Salt-Tolerance
salt tolerance
Magnesium
transporters
magnesium
Salts
Xylem
rice
Phloem
sap
Spatial Analysis
shoots
salt stress
xylem
Meristem
Xenopus laevis
salts
Oocytes
root tips
leaf blade

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

A magnesium transporter OsMGT1 plays a critical role in salt tolerance in rice. / Chen, Zhi Chang; Yamaji, Naoki; Horie, Tomoaki; Che, Jing; Li, Jian; An, Gynheung; Ma, Jian Feng.

In: Plant Physiology, Vol. 174, No. 3, 01.07.2017, p. 1837-1849.

Research output: Contribution to journalArticle

Chen, Zhi Chang ; Yamaji, Naoki ; Horie, Tomoaki ; Che, Jing ; Li, Jian ; An, Gynheung ; Ma, Jian Feng. / A magnesium transporter OsMGT1 plays a critical role in salt tolerance in rice. In: Plant Physiology. 2017 ; Vol. 174, No. 3. pp. 1837-1849.
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