A lymphoid cell-specific nuclear factor containing c-rel-like proteins preferentially interacts with interleukin-6 κB-related motifs whose activities are repressed in lymphoid cells

Kohzo Nakayama, Hiroko Shimizu, Katsuyuki Mitomou, Tomoo Watanabe, Shu Ichi Okamoto, Ken-ichi Yamamoto

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The poroto-oncoprotein c-Rel is a member of the nuclear factor κB transcription factor family, which includes the p50 and p65 subunits of nuclear factor κB. We show here that c-Rel binds to κB sites as homodimers as well as heterodimers with P50. These homodimers and heterodimers show distinct DNA-binding specificities and affinities for various κB motifs. In particular, the c-Rel homodimer has a high affinity for interleukin-6 (IL-6) and bete interferon κB sites. In spite of its association with p50 in vitro, however, we found a lymphoid cell-specific nuclear factor in vivo that contains c-Rel but not P50 epitopes; this factor, termed IL-6κB binding factor II, appears to contain the c-Rel homodimer and preferentially recognizes several IL-6κB-related κB motifs. Although it has been previously shown that the IL-6κB motif functions as a potent IL-1/tumor necrosis factor-responsive element in nonlymphoid cells, its activity was found to be repressed in lymphoid cells such as a Jurkat T-cell line. We also present evidence that IL-6κB binding factor II functions as a repressor specific for IL-6κB-related κB motifs in lymphoid cells.

Original languageEnglish
Pages (from-to)1736-1746
Number of pages11
JournalMolecular and Cellular Biology
Volume12
Issue number4
Publication statusPublished - Apr 1992
Externally publishedYes

Fingerprint

Proto-Oncogene Proteins c-rel
Interleukin-6
Lymphocytes
Jurkat Cells
Oncogene Proteins
Interleukin-1
Interferons
Epitopes
Transcription Factors
Tumor Necrosis Factor-alpha
T-Lymphocytes
Cell Line
DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

A lymphoid cell-specific nuclear factor containing c-rel-like proteins preferentially interacts with interleukin-6 κB-related motifs whose activities are repressed in lymphoid cells. / Nakayama, Kohzo; Shimizu, Hiroko; Mitomou, Katsuyuki; Watanabe, Tomoo; Okamoto, Shu Ichi; Yamamoto, Ken-ichi.

In: Molecular and Cellular Biology, Vol. 12, No. 4, 04.1992, p. 1736-1746.

Research output: Contribution to journalArticle

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