A loss-of-function allele of OsHMA3 associated with high cadmium accumulation in shoots and grain of Japonica rice cultivars

Jiali Yan, Peitong Wang, Peng Wang, Meng Yang, Xingming Lian, Zhong Tang, Chao Feng Huang, David E. Salt, Fang Jie Zhao

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Excessive cadmium (Cd) accumulation in rice poses a risk to food safety. OsHMA3 plays an important role in restricting Cd translocation from roots to shoots. A non-functional allele of OsHMA3 has been reported in some Indica rice cultivars with high Cd accumulation, but it is not known if OsHMA3 allelic variation is associated with Cd accumulation in Japonica cultivars. In this study, we identified a Japonica cultivar with consistently high Cd accumulation in shoots and grain in both field and greenhouse experiments. The cultivar possesses an OsHMA3 allele with a predicted amino acid mutation at the 380 th position from Ser to Arg. The haplotype had no Cd transport activity when the gene was expressed in yeast, and the allele did not complement a known nonfunctional allele of OsHMA3 in F 1 test. The allele is present only in temperate Japonica cultivars among diversity panels of 1483 rice cultivars. Different cultivars possessing this allele showed greatly increased root-to-shoot Cd translocation and a shift in root Cd speciation from Cd―S to Cd―O bonding determined by synchrotron X-ray absorption spectroscopy. Our study has identified a new loss-of-function allele of OsHMA3 in Japonica rice cultivars leading to high Cd accumulation in shoots and grain.

Original languageEnglish
Pages (from-to)1941-1954
Number of pages14
JournalPlant Cell and Environment
Volume39
Issue number9
DOIs
Publication statusPublished - Sep 1 2016
Externally publishedYes

Fingerprint

Cadmium
cadmium
Alleles
alleles
rice
shoots
cultivars
X-Ray Absorption Spectroscopy
Oryza
Synchrotrons
Food Safety
greenhouse experimentation
Haplotypes
food safety
haplotypes
complement
Yeasts
yeasts
mutation
Amino Acids

Keywords

  • allelic variation
  • Cd speciation
  • Oryza sativa (rice)
  • translocation

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

A loss-of-function allele of OsHMA3 associated with high cadmium accumulation in shoots and grain of Japonica rice cultivars. / Yan, Jiali; Wang, Peitong; Wang, Peng; Yang, Meng; Lian, Xingming; Tang, Zhong; Huang, Chao Feng; Salt, David E.; Zhao, Fang Jie.

In: Plant Cell and Environment, Vol. 39, No. 9, 01.09.2016, p. 1941-1954.

Research output: Contribution to journalArticle

Yan, Jiali ; Wang, Peitong ; Wang, Peng ; Yang, Meng ; Lian, Xingming ; Tang, Zhong ; Huang, Chao Feng ; Salt, David E. ; Zhao, Fang Jie. / A loss-of-function allele of OsHMA3 associated with high cadmium accumulation in shoots and grain of Japonica rice cultivars. In: Plant Cell and Environment. 2016 ; Vol. 39, No. 9. pp. 1941-1954.
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