TY - JOUR
T1 - A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus
AU - Hakata, Yoshiyuki
AU - Michiue, Hiroyuki
AU - Ohtsuki, Takashi
AU - Miyazawa, Masaaki
AU - Kitamatsu, Mizuki
N1 - Funding Information:
This work was supported by Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number JP16K05856 and JP16KT0195 . We thank Y. Kabata, N. Terada, T. Ishizuka for experimental support.
Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2019/4/1
Y1 - 2019/4/1
N2 - We synthesized a pair of compounds containing leucine zipper peptides to deliver protein cargo into cells. One is a cell-penetrating peptide (CPP) with Lz(E), a leucine zipper peptide containing negatively charged amino acids, and the other is a Nanog protein with Lz(K), a leucine zipper peptide containing positively charged amino acids. When cells were treated with these equimolar mixtures, Nanog-Lz(K) hybridized with Lz(E)-CPP was successfully delivered into the cells. Furthermore, Nanog-Lz(K) exerted its proper function after nuclear transport.
AB - We synthesized a pair of compounds containing leucine zipper peptides to deliver protein cargo into cells. One is a cell-penetrating peptide (CPP) with Lz(E), a leucine zipper peptide containing negatively charged amino acids, and the other is a Nanog protein with Lz(K), a leucine zipper peptide containing positively charged amino acids. When cells were treated with these equimolar mixtures, Nanog-Lz(K) hybridized with Lz(E)-CPP was successfully delivered into the cells. Furthermore, Nanog-Lz(K) exerted its proper function after nuclear transport.
KW - Cell-penetrating peptide
KW - Drug delivery system
KW - Leucine zipper
KW - Nanog
KW - Peptide
UR - http://www.scopus.com/inward/record.url?scp=85060977862&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85060977862&partnerID=8YFLogxK
U2 - 10.1016/j.bmcl.2019.02.004
DO - 10.1016/j.bmcl.2019.02.004
M3 - Article
C2 - 30737088
AN - SCOPUS:85060977862
SN - 0960-894X
VL - 29
SP - 878
EP - 881
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 7
ER -