Abstract
We synthesized a pair of compounds containing leucine zipper peptides to deliver protein cargo into cells. One is a cell-penetrating peptide (CPP) with Lz(E), a leucine zipper peptide containing negatively charged amino acids, and the other is a Nanog protein with Lz(K), a leucine zipper peptide containing positively charged amino acids. When cells were treated with these equimolar mixtures, Nanog-Lz(K) hybridized with Lz(E)-CPP was successfully delivered into the cells. Furthermore, Nanog-Lz(K) exerted its proper function after nuclear transport.
| Original language | English |
|---|---|
| Journal | Bioorganic and Medicinal Chemistry Letters |
| DOIs | |
| Publication status | Published - Jan 1 2019 |
Keywords
- Cell-penetrating peptide
- Drug delivery system
- Leucine zipper
- Nanog
- Peptide
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Pharmaceutical Science
- Drug Discovery
- Clinical Biochemistry
- Organic Chemistry
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Hakata, Y., Michiue, H., Ohtsuki, T., Miyazawa, M., & Kitamatsu, M. (2019). A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus. Bioorganic and Medicinal Chemistry Letters. https://doi.org/10.1016/j.bmcl.2019.02.004