A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus

Yoshiyuki Hakata; Hiroyuki Michiue; Takashi Ohtsuki; Masaaki Miyazawa; Mizuki Kitamatsu

doi:10.1016/j.bmcl.2019.02.004

A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus

Yoshiyuki Hakata, Hiroyuki Michiue, Takashi Ohtsuki, Masaaki Miyazawa, Mizuki Kitamatsu

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We synthesized a pair of compounds containing leucine zipper peptides to deliver protein cargo into cells. One is a cell-penetrating peptide (CPP) with Lz(E), a leucine zipper peptide containing negatively charged amino acids, and the other is a Nanog protein with Lz(K), a leucine zipper peptide containing positively charged amino acids. When cells were treated with these equimolar mixtures, Nanog-Lz(K) hybridized with Lz(E)-CPP was successfully delivered into the cells. Furthermore, Nanog-Lz(K) exerted its proper function after nuclear transport.

Original language	English
Pages (from-to)	878-881
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	29
Issue number	7
DOIs	https://doi.org/10.1016/j.bmcl.2019.02.004
Publication status	Published - Apr 1 2019

Keywords

Cell-penetrating peptide
Drug delivery system
Leucine zipper
Nanog
Peptide

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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title = "A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus",

abstract = "We synthesized a pair of compounds containing leucine zipper peptides to deliver protein cargo into cells. One is a cell-penetrating peptide (CPP) with Lz(E), a leucine zipper peptide containing negatively charged amino acids, and the other is a Nanog protein with Lz(K), a leucine zipper peptide containing positively charged amino acids. When cells were treated with these equimolar mixtures, Nanog-Lz(K) hybridized with Lz(E)-CPP was successfully delivered into the cells. Furthermore, Nanog-Lz(K) exerted its proper function after nuclear transport.",

keywords = "Cell-penetrating peptide, Drug delivery system, Leucine zipper, Nanog, Peptide",

author = "Yoshiyuki Hakata and Hiroyuki Michiue and Takashi Ohtsuki and Masaaki Miyazawa and Mizuki Kitamatsu",

note = "Funding Information: This work was supported by Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number JP16K05856 and JP16KT0195 . We thank Y. Kabata, N. Terada, T. Ishizuka for experimental support. Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

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AU - Hakata, Yoshiyuki

AU - Michiue, Hiroyuki

AU - Ohtsuki, Takashi

AU - Miyazawa, Masaaki

AU - Kitamatsu, Mizuki

N1 - Funding Information: This work was supported by Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number JP16K05856 and JP16KT0195 . We thank Y. Kabata, N. Terada, T. Ishizuka for experimental support. Publisher Copyright: © 2019 Elsevier Ltd

PY - 2019/4/1

Y1 - 2019/4/1

N2 - We synthesized a pair of compounds containing leucine zipper peptides to deliver protein cargo into cells. One is a cell-penetrating peptide (CPP) with Lz(E), a leucine zipper peptide containing negatively charged amino acids, and the other is a Nanog protein with Lz(K), a leucine zipper peptide containing positively charged amino acids. When cells were treated with these equimolar mixtures, Nanog-Lz(K) hybridized with Lz(E)-CPP was successfully delivered into the cells. Furthermore, Nanog-Lz(K) exerted its proper function after nuclear transport.

AB - We synthesized a pair of compounds containing leucine zipper peptides to deliver protein cargo into cells. One is a cell-penetrating peptide (CPP) with Lz(E), a leucine zipper peptide containing negatively charged amino acids, and the other is a Nanog protein with Lz(K), a leucine zipper peptide containing positively charged amino acids. When cells were treated with these equimolar mixtures, Nanog-Lz(K) hybridized with Lz(E)-CPP was successfully delivered into the cells. Furthermore, Nanog-Lz(K) exerted its proper function after nuclear transport.

KW - Cell-penetrating peptide

KW - Drug delivery system

KW - Leucine zipper

KW - Nanog

KW - Peptide

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JO - Bioorganic and Medicinal Chemistry Letters

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A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus

Abstract

Keywords

ASJC Scopus subject areas

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