A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus

Yoshiyuki Hakata, Hiroyuki Michiue, Takashi Ohtsuki, Masaaki Miyazawa, Mizuki Kitamatsu

Research output: Contribution to journal › Article

Abstract

We synthesized a pair of compounds containing leucine zipper peptides to deliver protein cargo into cells. One is a cell-penetrating peptide (CPP) with Lz(E), a leucine zipper peptide containing negatively charged amino acids, and the other is a Nanog protein with Lz(K), a leucine zipper peptide containing positively charged amino acids. When cells were treated with these equimolar mixtures, Nanog-Lz(K) hybridized with Lz(E)-CPP was successfully delivered into the cells. Furthermore, Nanog-Lz(K) exerted its proper function after nuclear transport.

Original languageEnglish
JournalBioorganic and Medicinal Chemistry Letters
DOIs
Publication statusPublished - Jan 1 2019

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Leucine Zippers
Cell Nucleus
Cell-Penetrating Peptides
Cells
Peptides
Amino Acids
Proteins
Cell Nucleus Active Transport
Nanog Homeobox Protein

Keywords

  • Cell-penetrating peptide
  • Drug delivery system
  • Leucine zipper
  • Nanog
  • Peptide

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus. / Hakata, Yoshiyuki; Michiue, Hiroyuki; Ohtsuki, Takashi; Miyazawa, Masaaki; Kitamatsu, Mizuki.

In: Bioorganic and Medicinal Chemistry Letters, 01.01.2019.

Research output: Contribution to journal › Article

Hakata, Y, Michiue, H, Ohtsuki, T, Miyazawa, M & Kitamatsu, M 2019, 'A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus', Bioorganic and Medicinal Chemistry Letters. https://doi.org/10.1016/j.bmcl.2019.02.004
Hakata Y, Michiue H, Ohtsuki T, Miyazawa M, Kitamatsu M. A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus. Bioorganic and Medicinal Chemistry Letters. 2019 Jan 1. https://doi.org/10.1016/j.bmcl.2019.02.004
Hakata, Yoshiyuki ; Michiue, Hiroyuki ; Ohtsuki, Takashi ; Miyazawa, Masaaki ; Kitamatsu, Mizuki. / A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus. In: Bioorganic and Medicinal Chemistry Letters. 2019.
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