A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein

M. Noda, T. Asai, Toshinori Shimanouchi, K. Yamashita, H. Umakoshi, M. Okuyama, R. Kubo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages317-319
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 2009
Externally publishedYes
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: Nov 1 2009Nov 5 2009

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period11/1/0911/5/09

Fingerprint

Carbonic anhydrase
Microsensors
Carbonic Anhydrases
Liposomes
Leakage currents
Electrolytes
Biomolecules
Proteins
Micromachining

Keywords

  • Electrolyte
  • Interaction
  • Leakage current
  • Liposome
  • Protein

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Noda, M., Asai, T., Shimanouchi, T., Yamashita, K., Umakoshi, H., Okuyama, M., & Kubo, R. (2009). A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 317-319). Chemical and Biological Microsystems Society.

A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein. / Noda, M.; Asai, T.; Shimanouchi, Toshinori; Yamashita, K.; Umakoshi, H.; Okuyama, M.; Kubo, R.

Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 317-319.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Noda, M, Asai, T, Shimanouchi, T, Yamashita, K, Umakoshi, H, Okuyama, M & Kubo, R 2009, A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein. in Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 317-319, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, Jeju, Korea, Republic of, 11/1/09.
Noda M, Asai T, Shimanouchi T, Yamashita K, Umakoshi H, Okuyama M et al. A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2009. p. 317-319
Noda, M. ; Asai, T. ; Shimanouchi, Toshinori ; Yamashita, K. ; Umakoshi, H. ; Okuyama, M. ; Kubo, R. / A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. pp. 317-319
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