TY - GEN
T1 - A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein
AU - Noda, Minoru
AU - Asai, Takeshi
AU - Yamashita, Kaoru
AU - Shimanouchi, Toshinori
AU - Umakoshi, Hiroshi
AU - Okuyama, Masanori
AU - Kuboi, Ryouichi
N1 - Funding Information:
This research was supported in part by Grants-in-Aid for Scientific Research (No. 19360162, 21246121, 20760539) from the Japan Society for the Promotion of Science. It was partly supported by the Core University Program between JSPS and VAST.
PY - 2009
Y1 - 2009
N2 - We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surfacebulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.
AB - We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surfacebulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.
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U2 - 10.1109/ICSENS.2009.5398390
DO - 10.1109/ICSENS.2009.5398390
M3 - Conference contribution
AN - SCOPUS:77951095644
SN - 9781424445486
T3 - Proceedings of IEEE Sensors
SP - 1881
EP - 1884
BT - IEEE Sensors 2009 Conference - SENSORS 2009
T2 - IEEE Sensors 2009 Conference - SENSORS 2009
Y2 - 25 October 2009 through 28 October 2009
ER -