A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein

Minoru Noda, Takeshi Asai, Kaoru Yamashita, Toshinori Shimanouchi, Hiroshi Umakoshi, Masanori Okuyama, Ryouichi Kuboi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surfacebulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.

Original languageEnglish
Title of host publicationProceedings of IEEE Sensors
Pages1881-1884
Number of pages4
DOIs
Publication statusPublished - 2009
Externally publishedYes
EventIEEE Sensors 2009 Conference - SENSORS 2009 - Christchurch, New Zealand
Duration: Oct 25 2009Oct 28 2009

Other

OtherIEEE Sensors 2009 Conference - SENSORS 2009
CountryNew Zealand
CityChristchurch
Period10/25/0910/28/09

Fingerprint

Carbonic anhydrase
Microsensors
Liposomes
Leakage currents
Electrolytes
Biomolecules
Proteins
Micromachining

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Noda, M., Asai, T., Yamashita, K., Shimanouchi, T., Umakoshi, H., Okuyama, M., & Kuboi, R. (2009). A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein. In Proceedings of IEEE Sensors (pp. 1881-1884). [5398390] https://doi.org/10.1109/ICSENS.2009.5398390

A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein. / Noda, Minoru; Asai, Takeshi; Yamashita, Kaoru; Shimanouchi, Toshinori; Umakoshi, Hiroshi; Okuyama, Masanori; Kuboi, Ryouichi.

Proceedings of IEEE Sensors. 2009. p. 1881-1884 5398390.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Noda, M, Asai, T, Yamashita, K, Shimanouchi, T, Umakoshi, H, Okuyama, M & Kuboi, R 2009, A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein. in Proceedings of IEEE Sensors., 5398390, pp. 1881-1884, IEEE Sensors 2009 Conference - SENSORS 2009, Christchurch, New Zealand, 10/25/09. https://doi.org/10.1109/ICSENS.2009.5398390
Noda M, Asai T, Yamashita K, Shimanouchi T, Umakoshi H, Okuyama M et al. A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein. In Proceedings of IEEE Sensors. 2009. p. 1881-1884. 5398390 https://doi.org/10.1109/ICSENS.2009.5398390
Noda, Minoru ; Asai, Takeshi ; Yamashita, Kaoru ; Shimanouchi, Toshinori ; Umakoshi, Hiroshi ; Okuyama, Masanori ; Kuboi, Ryouichi. / A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein. Proceedings of IEEE Sensors. 2009. pp. 1881-1884
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