A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein

Minoru Noda, Takeshi Asai, Kaoru Yamashita, Toshinori Shimanouchi, Hiroshi Umakoshi, Masanori Okuyama, Ryouichi Kuboi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surfacebulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.

Original languageEnglish
Title of host publicationIEEE Sensors 2009 Conference - SENSORS 2009
Pages1881-1884
Number of pages4
DOIs
Publication statusPublished - Dec 1 2009
Externally publishedYes
EventIEEE Sensors 2009 Conference - SENSORS 2009 - Christchurch, New Zealand
Duration: Oct 25 2009Oct 28 2009

Publication series

NameProceedings of IEEE Sensors

Other

OtherIEEE Sensors 2009 Conference - SENSORS 2009
CountryNew Zealand
CityChristchurch
Period10/25/0910/28/09

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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    Noda, M., Asai, T., Yamashita, K., Shimanouchi, T., Umakoshi, H., Okuyama, M., & Kuboi, R. (2009). A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein. In IEEE Sensors 2009 Conference - SENSORS 2009 (pp. 1881-1884). [5398390] (Proceedings of IEEE Sensors). https://doi.org/10.1109/ICSENS.2009.5398390