TY - GEN
T1 - A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein
AU - Noda, Minoru
AU - Asai, Takeshi
AU - Yamashita, Kaoru
AU - Shimanouchi, Toshinori
AU - Umakoshi, Hiroshi
AU - Okuyama, Masanori
AU - Kuboi, Ryouichi
PY - 2009/12/1
Y1 - 2009/12/1
N2 - We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surfacebulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.
AB - We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surfacebulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.
UR - http://www.scopus.com/inward/record.url?scp=77951095644&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77951095644&partnerID=8YFLogxK
U2 - 10.1109/ICSENS.2009.5398390
DO - 10.1109/ICSENS.2009.5398390
M3 - Conference contribution
AN - SCOPUS:77951095644
SN - 9781424445486
T3 - Proceedings of IEEE Sensors
SP - 1881
EP - 1884
BT - IEEE Sensors 2009 Conference - SENSORS 2009
T2 - IEEE Sensors 2009 Conference - SENSORS 2009
Y2 - 25 October 2009 through 28 October 2009
ER -