A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein

Minoru Noda; Takeshi Asai; Kaoru Yamashita; Toshinori Shimanouchi; Hiroshi Umakoshi; Masanori Okuyama; Ryouichi Kuboi

doi:10.1109/ICSENS.2009.5398390

A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein

Minoru Noda, Takeshi Asai, Kaoru Yamashita, Toshinori Shimanouchi, Hiroshi Umakoshi, Masanori Okuyama, Ryouichi Kuboi

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Citations (Scopus)

Abstract

We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surfacebulk-micromachining process. K₄[Fe(CN)₆] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.

Original language	English
Title of host publication	IEEE Sensors 2009 Conference - SENSORS 2009
Pages	1881-1884
Number of pages	4
DOIs	https://doi.org/10.1109/ICSENS.2009.5398390
Publication status	Published - Dec 1 2009
Externally published	Yes
Event	IEEE Sensors 2009 Conference - SENSORS 2009 - Christchurch, New Zealand Duration: Oct 25 2009 → Oct 28 2009

Publication series

Name	Proceedings of IEEE Sensors

Other

Other	IEEE Sensors 2009 Conference - SENSORS 2009
Country	New Zealand
City	Christchurch
Period	10/25/09 → 10/28/09

ASJC Scopus subject areas

Electrical and Electronic Engineering

Access to Document

10.1109/ICSENS.2009.5398390

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A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein. / Noda, Minoru; Asai, Takeshi; Yamashita, Kaoru; Shimanouchi, Toshinori; Umakoshi, Hiroshi; Okuyama, Masanori; Kuboi, Ryouichi.

IEEE Sensors 2009 Conference - SENSORS 2009. 2009. p. 1881-1884 5398390 (Proceedings of IEEE Sensors).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Noda, M, Asai, T, Yamashita, K, Shimanouchi, T, Umakoshi, H, Okuyama, M & Kuboi, R 2009, A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein. in IEEE Sensors 2009 Conference - SENSORS 2009., 5398390, Proceedings of IEEE Sensors, pp. 1881-1884, IEEE Sensors 2009 Conference - SENSORS 2009, Christchurch, New Zealand, 10/25/09. https://doi.org/10.1109/ICSENS.2009.5398390

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abstract = "We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surfacebulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.",

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AU - Umakoshi, Hiroshi

AU - Okuyama, Masanori

AU - Kuboi, Ryouichi

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