Abstract
We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surfacebulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of IEEE Sensors |
| Pages | 1881-1884 |
| Number of pages | 4 |
| DOIs | |
| Publication status | Published - 2009 |
| Externally published | Yes |
| Event | IEEE Sensors 2009 Conference - SENSORS 2009 - Christchurch, New Zealand Duration: Oct 25 2009 → Oct 28 2009 |
Other
| Other | IEEE Sensors 2009 Conference - SENSORS 2009 |
|---|---|
| Country | New Zealand |
| City | Christchurch |
| Period | 10/25/09 → 10/28/09 |
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ASJC Scopus subject areas
- Electrical and Electronic Engineering
Cite this
A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein. / Noda, Minoru; Asai, Takeshi; Yamashita, Kaoru; Shimanouchi, Toshinori; Umakoshi, Hiroshi; Okuyama, Masanori; Kuboi, Ryouichi.
Proceedings of IEEE Sensors. 2009. p. 1881-1884 5398390.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - A leakage current microsensor for detection of interaction between an electrolyte-entrapping liposome and protein
AU - Noda, Minoru
AU - Asai, Takeshi
AU - Yamashita, Kaoru
AU - Shimanouchi, Toshinori
AU - Umakoshi, Hiroshi
AU - Okuyama, Masanori
AU - Kuboi, Ryouichi
PY - 2009
Y1 - 2009
N2 - We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surfacebulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.
AB - We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surfacebulk-micromachining process. K4[Fe(CN)6] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.
UR - http://www.scopus.com/inward/record.url?scp=77951095644&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77951095644&partnerID=8YFLogxK
U2 - 10.1109/ICSENS.2009.5398390
DO - 10.1109/ICSENS.2009.5398390
M3 - Conference contribution
AN - SCOPUS:77951095644
SN - 9781424445486
SP - 1881
EP - 1884
BT - Proceedings of IEEE Sensors
ER -