A ketolide antibiotic, telithromycin, inhibits vascular adrenergic neurotransmission in the rat mesenteric vascular bed

Y. Hatanaka, Y. Zamami, Toshihiro Koyama, N. Hobara, X. Jin, Yoshihisa Kitamura, H. Kawasaki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background and purpose: A ketolide antibiotic, telithromycin, has side effects including temporary loss of consciousness in clinical use, but the underlying mechanisms remain unclear. This study investigated the effects of telithromycin on perivascular nerve function in rat mesenteric arteries, in comparison with those of macrolide (erythromycin and clarithromycin) and new quinolone antibiotics (levofloxacin and gatifloxacin). Experimental approach: In vitro, vascular responses and release of noradrenaline induced by periarterial nerve stimulation (PNS) of rat perfused mesenteric vascular beds were measured in the presence of each antibiotic. In vivo blood pressure measurement was performed in Wistar rats. Key results: In mesenteric preparations with resting tone, telithromycin (10 nM-10 μM) markedly inhibited PNS (4-12 Hz)-induced adrenergic nerve- and exogenous noradrenaline-mediated vasoconstriction, whereas the other antibiotics slightly inhibited PNS-induced responses without affecting noradrenaline-induced responses. Telithromycin significantly reduced PNS (12 Hz)-evoked noradrenaline release in the perfusate. In pre-constricted preparations with or without endothelium, telithromycin (0.1 nM-10 μM) caused a concentration-dependent vasodilation. Telithromycin (10 nM) inhibited calcium-induced vasoconstriction in high KCl and calcium-free medium. None of the antibiotics used affected PNS (0.5-2 Hz)-induced calcitonin gene-related peptide (CGRP) nerve- and exogenous CGRP-mediated vasodilation. Intravenous injection of telithromycin significantly lowered blood pressure in anaesthetized rats. Conclusions and implications: These results suggest that telithromycin causes not only strong inhibition of perivascular adrenergic neurotransmission but also a vasodilator action in mesenteric vascular beds and hypotension. It is thus possible that telithromycin increases visceral blood flow, consequently reducing cerebral blood flow and resulting in a temporary loss of consciousness.

Original languageEnglish
Pages (from-to)826-836
Number of pages11
JournalBritish Journal of Pharmacology
Volume155
Issue number6
DOIs
Publication statusPublished - Nov 2008

Fingerprint

Ketolides
Synaptic Transmission
Adrenergic Agents
Blood Vessels
Anti-Bacterial Agents
Norepinephrine
Unconsciousness
Calcitonin Gene-Related Peptide
Vasoconstriction
Vasodilation
Cerebrovascular Circulation
Blood Pressure
Calcium
telithromycin
Levofloxacin
Mesenteric Arteries
Clarithromycin
Quinolones
Macrolides
Erythromycin

Keywords

  • Adrenergic nerve neurotransmission
  • Calcitonin gene-related peptide-containing nerves
  • Ketolide antibiotics
  • Rat mesenteric arteries
  • Telithromycin
  • Vasodilation

ASJC Scopus subject areas

  • Pharmacology

Cite this

A ketolide antibiotic, telithromycin, inhibits vascular adrenergic neurotransmission in the rat mesenteric vascular bed. / Hatanaka, Y.; Zamami, Y.; Koyama, Toshihiro; Hobara, N.; Jin, X.; Kitamura, Yoshihisa; Kawasaki, H.

In: British Journal of Pharmacology, Vol. 155, No. 6, 11.2008, p. 826-836.

Research output: Contribution to journalArticle

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AU - Kitamura, Yoshihisa

AU - Kawasaki, H.

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