A hybrid protein between IFN-γ and IL-2

Masaharu Seno, Shuji Hinuma, Haruo Onda, Koichi Igarashi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The complementary DNAs encoding human interferon-γ (IFN-γ) and human interleukin-2 (IL-2), two different proteins involved in the same immune system, were fused to code a hybrid protein, which was expressed in E. coli to investigate the interactions of these two proteins at the molecular level. Through immunoprecipitation analysis, this protein was revealed to be of about 31 kDa, which was expected from nucleotide sequencing, and to have the antigenicities of both IFN-γ and IL-2. The extract from bacteria expressing this hybrid protein showed at least two biological activities: an antiviral activity derived from IFN-γ and the ability to support the growth of natural killer (NK) cells derived from IL-2. Comparing the enhancement of NK cell activity of this hybrid protein with IFN-γ and IL-2, this hybrid protein appears to conserve each activity almost completely without diminishing the other.

Original languageEnglish
Pages (from-to)187-192
Number of pages6
JournalFEBS Letters
Volume199
Issue number2
DOIs
Publication statusPublished - Apr 21 1986
Externally publishedYes

Keywords

  • (Human)
  • Antigenicity
  • Hybrid protein
  • Integrated biological activity
  • Interferon-γ
  • Interleukin-2
  • complementary DNA

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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