A hybrid protein between IFN-γ and IL-2

Masaharu Seno, Shuji Hinuma, Haruo Onda, Koichi Igarashi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The complementary DNAs encoding human interferon-γ (IFN-γ) and human interleukin-2 (IL-2), two different proteins involved in the same immune system, were fused to code a hybrid protein, which was expressed in E. coli to investigate the interactions of these two proteins at the molecular level. Through immunoprecipitation analysis, this protein was revealed to be of about 31 kDa, which was expected from nucleotide sequencing, and to have the antigenicities of both IFN-γ and IL-2. The extract from bacteria expressing this hybrid protein showed at least two biological activities: an antiviral activity derived from IFN-γ and the ability to support the growth of natural killer (NK) cells derived from IL-2. Comparing the enhancement of NK cell activity of this hybrid protein with IFN-γ and IL-2, this hybrid protein appears to conserve each activity almost completely without diminishing the other.

Original languageEnglish
Pages (from-to)187-192
Number of pages6
JournalFEBS Letters
Volume199
Issue number2
DOIs
Publication statusPublished - Apr 21 1986
Externally publishedYes

Fingerprint

Interferons
Interleukin-2
Proteins
Natural Killer Cells
Immune system
Bioactivity
Immunoprecipitation
Escherichia coli
Antiviral Agents
Immune System
Bacteria
Nucleotides
Complementary DNA
Growth

Keywords

  • (Human)
  • Antigenicity
  • complementary DNA
  • Hybrid protein
  • Integrated biological activity
  • Interferon-γ
  • Interleukin-2

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A hybrid protein between IFN-γ and IL-2. / Seno, Masaharu; Hinuma, Shuji; Onda, Haruo; Igarashi, Koichi.

In: FEBS Letters, Vol. 199, No. 2, 21.04.1986, p. 187-192.

Research output: Contribution to journalArticle

Seno, M, Hinuma, S, Onda, H & Igarashi, K 1986, 'A hybrid protein between IFN-γ and IL-2', FEBS Letters, vol. 199, no. 2, pp. 187-192. https://doi.org/10.1016/0014-5793(86)80477-X
Seno, Masaharu ; Hinuma, Shuji ; Onda, Haruo ; Igarashi, Koichi. / A hybrid protein between IFN-γ and IL-2. In: FEBS Letters. 1986 ; Vol. 199, No. 2. pp. 187-192.
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