A hybrid protein between IFN-γ and IL-2

Masaharu Seno; Shuji Hinuma; Haruo Onda; Koichi Igarashi

doi:10.1016/0014-5793(86)80477-X

A hybrid protein between IFN-γ and IL-2

Masaharu Seno, Shuji Hinuma, Haruo Onda, Koichi Igarashi

Research output: Contribution to journal › Article

12 Citations (Scopus)

Abstract

The complementary DNAs encoding human interferon-γ (IFN-γ) and human interleukin-2 (IL-2), two different proteins involved in the same immune system, were fused to code a hybrid protein, which was expressed in E. coli to investigate the interactions of these two proteins at the molecular level. Through immunoprecipitation analysis, this protein was revealed to be of about 31 kDa, which was expected from nucleotide sequencing, and to have the antigenicities of both IFN-γ and IL-2. The extract from bacteria expressing this hybrid protein showed at least two biological activities: an antiviral activity derived from IFN-γ and the ability to support the growth of natural killer (NK) cells derived from IL-2. Comparing the enhancement of NK cell activity of this hybrid protein with IFN-γ and IL-2, this hybrid protein appears to conserve each activity almost completely without diminishing the other.

Original language	English
Pages (from-to)	187-192
Number of pages	6
Journal	FEBS Letters
Volume	199
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(86)80477-X
Publication status	Published - Apr 21 1986
Externally published	Yes

Keywords

(Human)
Antigenicity
Hybrid protein
Integrated biological activity
Interferon-γ
Interleukin-2
complementary DNA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(86)80477-X

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Seno, M., Hinuma, S., Onda, H., & Igarashi, K. (1986). A hybrid protein between IFN-γ and IL-2. FEBS Letters, 199(2), 187-192. https://doi.org/10.1016/0014-5793(86)80477-X

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abstract = "The complementary DNAs encoding human interferon-γ (IFN-γ) and human interleukin-2 (IL-2), two different proteins involved in the same immune system, were fused to code a hybrid protein, which was expressed in E. coli to investigate the interactions of these two proteins at the molecular level. Through immunoprecipitation analysis, this protein was revealed to be of about 31 kDa, which was expected from nucleotide sequencing, and to have the antigenicities of both IFN-γ and IL-2. The extract from bacteria expressing this hybrid protein showed at least two biological activities: an antiviral activity derived from IFN-γ and the ability to support the growth of natural killer (NK) cells derived from IL-2. Comparing the enhancement of NK cell activity of this hybrid protein with IFN-γ and IL-2, this hybrid protein appears to conserve each activity almost completely without diminishing the other.",

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author = "Masaharu Seno and Shuji Hinuma and Haruo Onda and Koichi Igarashi",

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AU - Seno, Masaharu

AU - Hinuma, Shuji

AU - Onda, Haruo

AU - Igarashi, Koichi

PY - 1986/4/21

Y1 - 1986/4/21

N2 - The complementary DNAs encoding human interferon-γ (IFN-γ) and human interleukin-2 (IL-2), two different proteins involved in the same immune system, were fused to code a hybrid protein, which was expressed in E. coli to investigate the interactions of these two proteins at the molecular level. Through immunoprecipitation analysis, this protein was revealed to be of about 31 kDa, which was expected from nucleotide sequencing, and to have the antigenicities of both IFN-γ and IL-2. The extract from bacteria expressing this hybrid protein showed at least two biological activities: an antiviral activity derived from IFN-γ and the ability to support the growth of natural killer (NK) cells derived from IL-2. Comparing the enhancement of NK cell activity of this hybrid protein with IFN-γ and IL-2, this hybrid protein appears to conserve each activity almost completely without diminishing the other.

AB - The complementary DNAs encoding human interferon-γ (IFN-γ) and human interleukin-2 (IL-2), two different proteins involved in the same immune system, were fused to code a hybrid protein, which was expressed in E. coli to investigate the interactions of these two proteins at the molecular level. Through immunoprecipitation analysis, this protein was revealed to be of about 31 kDa, which was expected from nucleotide sequencing, and to have the antigenicities of both IFN-γ and IL-2. The extract from bacteria expressing this hybrid protein showed at least two biological activities: an antiviral activity derived from IFN-γ and the ability to support the growth of natural killer (NK) cells derived from IL-2. Comparing the enhancement of NK cell activity of this hybrid protein with IFN-γ and IL-2, this hybrid protein appears to conserve each activity almost completely without diminishing the other.

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