A Homologue of N-Ethylmaleimide-sensitive Factor in the Malaria Parasite Plasmodium falciparum Is Exported and Localized in Vesicular Structures in the Cytoplasm of Infected Erythrocytes in the Brefeldin A-sensitive Pathway

Mitsuko Hayashi, Shinya Taniguchi, Yuki Ishizuka, Hye-Sook Kim, Yusuke Wataya, Akitsugu Yamamoto, Yoshinori Moriyama

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Abstract

N-Ethylmaleimide-sensitive factor (NSF) and its homologues play a central role in vesicular trafficking in eukaryotic cells. We have identified a NSF homologue in .Plasmodium falciparum (PfNSF). The reported PfNSF gene sequence (GenBank™ accession number CAB10575) indicated that PfNSF comprises 783 amino acids with a calculated molecular weight of 89,133. The overall identities of its gene and amino acid sequences with those of rat NSF are 50.9 and 48.8%, respectively. Reverse transcription-polymerase chain reaction analysis and Northern blotting with total P. falciparum RNA indicated expression of the PfNSF gene. Polyclonal an. tibodies against a conserved region of NSF specifically recognized an 89-kDa polypeptide in the parasite cells. After homogenization of the parasite cells, ∼90% of an 89-kDa polypeptide is associated with particulate fraction, suggesting membrane-bound nature of PfNSF. PfNSF was present within both the parasite cells and the vesicular structure outside of the parasite cells. The export of PfNSF outside of the parasite cells appears to occur at the early trophozoite stage and to terminate at the merozoite stage. The export of PfNSF is inhibited by brefeldin A, with 9 μM causing 50% inhibition. Immunoelectromicroscopy indicated that intracellular PfNSF was associated with organelles such as food vacuoles and that extracellular PfNSF was associated with vesic. ular structures in the erythrocyte cytoplasm. These results indicate that PfNSF expressed in the malaria parasite is exported to the extracellular space and then localized in intraerythrocytic vesicles in a brefeldin Asensitive manner. It is suggested that a vesicular trans. port mechanism is involved in protein export targeted to erythrocyte membranes during intraerythrocytic development of the malaria parasite.

Original languageEnglish
Pages (from-to)15249-15255
Number of pages7
JournalJournal of Biological Chemistry
Volume276
Issue number18
DOIs
Publication statusPublished - May 4 2001

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N-Ethylmaleimide-Sensitive Proteins
Brefeldin A
Falciparum Malaria
Cytoplasm
Parasites
Erythrocytes
Genes
Plasmodium falciparum
Malaria
Membranes
Merozoites
Amino Acids
Trophozoites
Peptides
Polymerase chain reaction
Nucleic Acid Databases
Extracellular Space
Erythrocyte Membrane
Eukaryotic Cells
Transcription

ASJC Scopus subject areas

  • Biochemistry

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A Homologue of N-Ethylmaleimide-sensitive Factor in the Malaria Parasite Plasmodium falciparum Is Exported and Localized in Vesicular Structures in the Cytoplasm of Infected Erythrocytes in the Brefeldin A-sensitive Pathway. / Hayashi, Mitsuko; Taniguchi, Shinya; Ishizuka, Yuki; Kim, Hye-Sook; Wataya, Yusuke; Yamamoto, Akitsugu; Moriyama, Yoshinori.

In: Journal of Biological Chemistry, Vol. 276, No. 18, 04.05.2001, p. 15249-15255.

Research output: Contribution to journalArticle

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title = "A Homologue of N-Ethylmaleimide-sensitive Factor in the Malaria Parasite Plasmodium falciparum Is Exported and Localized in Vesicular Structures in the Cytoplasm of Infected Erythrocytes in the Brefeldin A-sensitive Pathway",
abstract = "N-Ethylmaleimide-sensitive factor (NSF) and its homologues play a central role in vesicular trafficking in eukaryotic cells. We have identified a NSF homologue in .Plasmodium falciparum (PfNSF). The reported PfNSF gene sequence (GenBank™ accession number CAB10575) indicated that PfNSF comprises 783 amino acids with a calculated molecular weight of 89,133. The overall identities of its gene and amino acid sequences with those of rat NSF are 50.9 and 48.8{\%}, respectively. Reverse transcription-polymerase chain reaction analysis and Northern blotting with total P. falciparum RNA indicated expression of the PfNSF gene. Polyclonal an. tibodies against a conserved region of NSF specifically recognized an 89-kDa polypeptide in the parasite cells. After homogenization of the parasite cells, ∼90{\%} of an 89-kDa polypeptide is associated with particulate fraction, suggesting membrane-bound nature of PfNSF. PfNSF was present within both the parasite cells and the vesicular structure outside of the parasite cells. The export of PfNSF outside of the parasite cells appears to occur at the early trophozoite stage and to terminate at the merozoite stage. The export of PfNSF is inhibited by brefeldin A, with 9 μM causing 50{\%} inhibition. Immunoelectromicroscopy indicated that intracellular PfNSF was associated with organelles such as food vacuoles and that extracellular PfNSF was associated with vesic. ular structures in the erythrocyte cytoplasm. These results indicate that PfNSF expressed in the malaria parasite is exported to the extracellular space and then localized in intraerythrocytic vesicles in a brefeldin Asensitive manner. It is suggested that a vesicular trans. port mechanism is involved in protein export targeted to erythrocyte membranes during intraerythrocytic development of the malaria parasite.",
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AU - Hayashi, Mitsuko

AU - Taniguchi, Shinya

AU - Ishizuka, Yuki

AU - Kim, Hye-Sook

AU - Wataya, Yusuke

AU - Yamamoto, Akitsugu

AU - Moriyama, Yoshinori

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